Gentaur now offers 30% discount on ELISA Kits:

Cat.	Product	Quantity	Old Price	Promo Price	Order
CSB-E15903Br	Avian Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit	96T	600 €	462 €
CSB-E16209	Biotin ELISA Kit	96T	696 €	535 €
CSB-EQ027738BO	Bovine Adenovirus 3 (ADV3) antibody (IgG) ELISA kit	96T	696 €	535 €
CSB-EL027852BO	Bovine anti-toxoplasmosis antibody(IgG) ELISA kit	96T	696 €	535 €
CSB-EL001918BO	Bovine Apolipoprotein B (APOB) ELISA kit	96T	696 €	535 €
CSB-EL001936BO	Bovine Apolipoprotein E(APOE) ELISA kit	96T	558 €	429 €
CSB-E15919B	Bovine C-Peptide ELISA Kit	96T	696 €	535 €
CSB-E13064B	Bovine Cortisol ELISA Kit	96T	506 €	389 €
CSB-E08173b	Bovine Estradiol,E2 ELISA Kit	96T	592 €	455 €
CSB-E13074B	Bovine folic acid,FA ELISA Kit	96T	696 €	535 €

More ELISA Kits from the promotion 

According to a new study intestinal bacteria play an important role in the development of white blood cells (leukocytes) that are associated with the immune system and the basic unit to fight infections that affect the body.

The human body is home to thousands of species of microbes, which under certain conditions can cause disease, but also have a number of benefits to our health qualities.

The highest concentration and the greatest diversity of bacterial species these sows findeth in the gastrointestinal tract and in particular in the colon.

A team from the California Institute of Technology (Caltech) in Pasadena, led by Sarkis Mazmanian, a professor of biology and biological engineering, has conducted research on this area of the human body.

Their work with mice has led to many discoveries about the importance of intestinal bacteria for health.

For example, in 2013, the team reported the relationship between microbes and autism, as well as their impact on the development of multiple sclerosis.

In a recent study published in the journal Cell Host & microbe, researchers describe their discovery that the beneficial intestinal bacteria play a key role in the development of innate immune cells - macrophages, monocytes and neutrophils - special white blood cells that provide the first line of defense against invading pathogens.

When the team comparing the number of white blood cells in these areas in mice born without intestinal bacteria - known as " non -microbial " mice and normal mice with normal colonic fluorines, they found that non - microbial mice had fewer white blood cells.

The team tested the ability of mice to combat infections by exposing them to a mono-cytogenetic bacterium Listeria.

Cells of the immune system are responsible for the maintenance of the immune reactivity of the organism against penetrating viral and bacterial pathogens in the human body, which sometimes can cause dangerous health infections. Initially, the circuit is driven by the production of pluripotent stem cells that differentiate and give rise to clones of B-and T-cell lymphocytes, neutrophils, eosinophils, basophils, monocytes, dendritic cells, mast cells and red blood cells.

Neutrophils are a special type of immune system cells, which are differentiated from myeloid stem cells. They migrate into the bloodstream at himiotaksichen signal from the body. By way of specific Fc-receptor fixed opsonized by the complement system and pathogen invasion within particles. They have the ability to destroy pathogens directly emptied by the strong bactericide them.

Eosinophils are another type of special cells of the immune system. These granules have a strong cytotoxicity to the endothelium, epidermis, neuronal cells and proteins. Their main function is to synthesize mediators and cytokines.

Basophil cells and mast cells contain receptors for immunoglobulin E. Their main function is to synthesize prostaglandins Class 2. They also provide protection against parasites come against immunoglobulin E-mediated allergic reactions. In this way, the body remains secure during falling of animal parasites in the food, which is particularly common in the consumption of food or poorly cleaned poorly processed meat products.

Monocytes have a cytoplasm filled with specific granules. They originate from myeloid stem cells and differentiate into macrophages. Their main function is to phagocytose emptied environmental pathogenic microorganisms. These processes are carried out after activation by interferon or TNF, macrophages subsequently acquire strong cytotoxic activity and have a strong bactericidal effect against pathogens become trapped in the body.

Dendritic cells are antigen presenting cells. They can migrate into the blood and lymph. They are activated by irritation of those who fell in the human pathogenic microorganisms. The next stage of their operation is not present on its surface MHC molecules of the system that is the major histocompatibility complex on. Also involved in the activation of clusters of differentiation in the body - CD-cells.

T lymphocytes very well may be defined as "the heads of special operations to eliminate the invasion and destruction of pathogenic microorganisms." They are produced in the bone marrow pluripotent stem cells and then migrate to the thymus and mature there. Subsequently differentiate and acquire the specific T-cell reactivity - TCR.

Accordingly, this type of cells are involved in the differentiation to a CD- cells , which are clusters of differentiation. CD4 + T - cell specific agents have adjuvant - inducing function . They give rise to a whole new spot in the production of protective cells against emptied into a person's body pathogenic microorganisms. CD8 + T-cell lymphocytes are the next most important clusters that need to be addressed. They have strong cytotoxic and suppressor role. Cast directly to the differentiation of killer cells and suppressor cells when the body is facing the threat of invasive blood-borne viruses and bacteria.

The role of the immune system is to protect the body against foreign antigens, and do not react to cause outbreaks of disease events in the human body. They also prevent the occurrence of cross-reactions between antigens and the invasion of the body's own antigens. That function of the immune system is performed by the T-cells that acquire the ability to execute it in the thymus after three-step reactions.

- The first step is differentiation of a double negative T cell lymphocytes. These are CD4-and CD8-cells.
- The second stage is the occurrence of double positive cells - to develop CD4 + and CD8 + cells.
- The third stage is the mixed cells, where they meet a single positive and single negative T-lymphocytes. There are two possible combinations. The first one is the manifestation of CD4-and CD8 + cells, and the second expression of CD4 + and CD8-cells.

B-cell lymphocytes are another important cellular arm of the immune reactivity of the organism against environmental emptied the invasion of microorganisms. They are involved in humoral immunity by synthesis of antibodies. Each mature B lymphocyte produces only one type specific antibody that is expressed on the surface, such as a B cell receptor. Antibodies react against the onset of the invasion antigens.

NK-cells are a specific cell type. They can be defined as "a SWAT team to fight pathogens." Their name comes from the abbreviation natural killers, which means that they kill directly taken into the body microorganisms without subjecting them to any attempts to phagocytic or neutralization. NK-cells are most pronounced cytotoxicity compared with all other members of the immune response. Thus limiting the spread of virus-infected cells in the human body.

Dr. Ivan Campeotto

MRC Centre for Molecular Bacteriology and Infection
Imperial College London
United Kingdom

In my current project at Imperial College London, I am focusing on several proteins from different human pathogens. One of these proteins forms crystals, which tend to be looped with the unique c axis of ~500Å in length and oriented in parallel with the X–ray beam. This led to a severe spot–overlapping problem, which was resolved with the aid of a kappa goniometer.
Gentaur products came to the rescue in different steps of this project.
The first issue to solve was the fact that crystals grew on layers of microcrystals mixed with protein skin, which, when disturbed with a traditional loop, would break into many pieces, enter the loop and thereby abolish diffraction. The Microloops™ allowed for precise selection of the desired crystals without perturbation of the drop, so that skin-free crystals inside the loop could be obtained.

Figure 1. Micro–crystals were used for in situ at Diamond beamline i04–1. X–ray beam and ruler are represented in red.Another problem was the reproducibility of the crystals, when crystallization trials were set–up in 96, 48 or 24 well plate formats.  I decided to try the new in–situ plates and to compare them with traditional 96 well, 48 well and 24 well plates. This experiment was performed on the same day and using the same protein batch. Whilst the other plates gave either only micro–crystals (<1μm) or failed completely to produce crystals within a given crystallization condition range, the Gentaur plates gave crystals of about 30μm in size (Fig.1)!

Figure 2. In situ diffraction at the Diamond Light Source synchrotron from Gentaur plate (beam line i04–1).In addition the Gentaur in situ plate was easily transported to the synchrotron (Diamond, beamline i04–1), as it could be packed vertically. Diffraction experiments were performed in situ and crystals diffracted approximately to 3.8Å (Fig.2) with a very low background diffraction compared to competitors.
In my opinion the greatest benefits were: crystal reproducibility, crystal–friendly plate transport and low background scattering. There are a few others benefits, which are not less important, such as the possibility of shooting the crystals with better orientations for data collection or the possibility of testing lattice disorders from data collected at RT vs. cryo data. These are all applications, which I am currently exploring at the i24 beamline at the Diamond Light Source (Oxford, UK), where complete data collection is currently achievable in situ.
We all know that obtaining crystals and manipulating crystals represent a continuous challenge; therefore I would like to thank Gentaur for producing innovative products, which assist us crystallographers not only in delivering better results but also in overcoming experimental obstacles, which otherwise could severely delay or hamper our work.

Gentaur’s In Situ 1 crystallization plates

Blood Coagulation Intermediates and Research Products

Gentaur has been a world leader in supplying Rabbit Brain Acetone Powder for use in blood clotting assays. Gentaur also offers its own patented recombinant Rabbit Tissue Factor protein (rRTF) for those interested in a ready-to-use protein in conjunction with synthetic lipids. Whether your instrument is a large multi-tasking analyzer, point-of-care, or personal instrument used at home, Gentaur has a variety of product configurations that can meet your needs in both the diagnostic and research communities.

Rabbit Brain Acetone Powder
Rabbit Brain Cephalin
Rabbit Plasma
Recombinant Rabbit Tissue Factor (rRTF)

Animal Blood Products

Gentaur provides animal blood products from a broad range of species, including bovine, goat, horse, pig, chicken and sheep, along with rabbit products from the largest USDA-inspected rabbit abattoir in the US. Products are processed to industry standard conditions or to meet your custom needs – sterile filtration, delipidization, heat inactivation – just to name a few available value add services. Large lot sizes and bulk pricing allow consistency and cost effectiveness for your production runs. Applications include but are not limited to, protein purification, cell culture and growth media, blocking reagents and diluents, carrier proteins, and coagulase assays.

Antibodies
Gamma Globulins
Plasma
Serum
Whole Blood

Complement and Ancillary Products

Gentaur has long been recognized by the World Health Organization as a supplier of 3-4 week rabbit complement for the SBA and MOPA functional antibody assays used in vaccine evaluation by some of the largest vaccine manufacturers in the world. It is also used upstream at the research and development level for cytotoxicity and cell depletion assays. Adult rabbit complement is a primary reagent used in tissue typing for organ transplantation and lymphocytoxicity testing. Gentaur features larges lots, consistent processing and extensive QC for reliable results. Ancillary products are offered for ease of one-stop ordering and shipping.

3-4 Week Rabbit Complement
Adult Rabbit Complement
Guinea Pig Complement
AB Human Serum

Animal Tissues and Tissue Powders/Extracts

Gentaur has decades of experience in collecting whole tissues from a variety of species – rabbits, bovine, pig and chicken are a few of the species offered. Or, if you need a special dissection or protocol, or a tissue powder or extract, Gentaur can do that for you, too. Our rabbit thymus powder is used for auto-immune antigen purification; our rabbit muscle powder for actin purification, and on and on. Our expertise will save you time and money, and get you to your discovery goal that much more quickly.

Bovine Lung Acetone Powder
Bovine Pituitary Extract
Chicken Pancreas Powder
Rabbit Muscle and Thymus Acetone Powder
Whole Organs, Glands & Tissues

Endothelial Cell Growth Medium Kit,  for enhanced, defined and serum-free growth conditions for endothelial cells from large vessels.

Cat no.:  XF501-0500

Kit Content:  500 ml medium + separate vials with supplements

Product Description:

XF501 Defined Endothelial Cell Growth Kit is a complete medium for the optimal growth of endothelial cells from large vessels. The kit contains a medium with essential and non-essential amino acids, vitamins, organic and inorganic compounds etc. Additionally the kit contains an optimized combination of growth factors, hormones and a growth supplement.

By using this kit for the growth of endothelial cells there is no need to use FBS or other sera. All components in the kit are free of animal and/or human derived materials.

The XF501 Defined Endothelial Cell Growth Kit is for in vitro research applications only.

More: Endothelial Cell Growth Kit

Speedy Breedy is an easy to use, compact portable, precision respirometer and monitor of microbial activity that has been designed for both industrial applications and lab research.

Speedy Breedy can be used:

Test raw ingredients and finished goods for contamination in beer and beverages
Shorten the time for positive release in food processing
Test for contamination in drinking water or process water in industrial applications
Test for contamination in pharmaceutical, household and hygiene products
Test effectiveness of decontamination processes following wet strip of all forms of equipment
Test sterility of food and beverage packaging
Identify the presence of bacteria in oil and paint
Test potentially detrimental contaminants, growth and metabolic promoters or inhibitors
Assist researchers to develop products and techniques where the presence or absence of bacteria needs to be understood, prevented or encouraged

Speedy Breedy is a single (test versus control or aerobic plus anaerobic), or dual sample, two chamber instrument. Speedy Breedy maintains culture conditions within purpose designed, disposable culture vessels. Samples are inoculated into the culture vessel and Speedy Breedy closely controls growth conditions to a pre-determined protocol in order to facilitate rapid replication of cells or microbes.

Speedy Breedy's robust, disposable 50 ml culture vessel eliminates the need for cleaning of potentially hazardous bottles. Culture vessels are provided sterile, empty or pre-filled with a range of dry media.

Speedy Breedy can be used independently or connected to a PC. Using the PC users can design and download pre-determined protocols to Speedy Breedy and upload experimental results for analysis. Speedy Breedy also has a removable SD card for field research. Connecting Speedy Breedy to a PC enables data visualization so that users can monitor experiments in near real time.

Featuries

• - Two temperature-controlled testing chambers
• - Purpose designed culture vessels with advanced mixing for optimised growth and detection of microbial activity
• - Sealed, easy to populate culture chambers
• - Suitable for use with a wide range of sample types
• - Culture vessels come empty or pre-filled with a range of dry media
• - Temperature controlled -5 below ambient to 44 degrees
• - A highly sensitive, non-invasive detection system that delivers rapid results
• - Barrier protected sensors for reliability and safety
• - PC (Windows compatible) software that enables near real time visualisation of experiments when connected to Speedy Breedy
• - Customised protocols designed off-line and downloaded to Speedy Breedy
• - Can be used independently for field research or in the lab connected to a PC
• - Enables visualisation of microbial activity in near real time
• - Does not require a large capital investment
• - Configurable to allow for comparative testing using a test vs. control protocol or two experiments
• - Speedy Breedy's robust, disposable culture vessels eliminate the need for cleaning of potentially hazardous bottles
• - On board 2GB SD card for experiment results storage
• - USB connection
  
  

• Software

Speedy Breedy turns microbial activity into data. When Speedy Breedy is connected to a PC the experiment is visualised in near real time as a graph.

Speedy Breedy comes pre-loaded with standard protocols that are designed to search for a "significant event";, that is when there is a significant pressure change in the headspace of the culture vessel, meaning there is microbial activity inside the culture chamber.

Speedy Breedy's Windows compatible software is intuitive and easy to use. Customers can design their own protocols on the PC and download them to Speedy Breedy. Protocols enable the user to set the incubation temperature (between 5 below ambient and 44 degrees), vary the time pressure readings are taken and the speed at which the mixing paddles rotate inside the culture vessel.

Experimental data can be uploaded from Speedy Breedy's SD card to the PC for archiving and further analysis.

How Speedy Breedy Works

Speedy Breedy is a portable, sensitive, precision respirometer which detects and monitors microbial activity. Detection of microbial activity is determined as a consequence of pressure transients relating to gaseous exchanges within a closed culture vessel of 50 ml working volume, as a result of microbial respiration.

Samples are added to a culture vessel containing a suitable medium and where present, contaminating microorganisms begin to grow. Speedy Breedy has advanced mixing technology that homogenises the culture conditions for rapid growth of microbes and efficiently converts gaseous changes in the culture medium into pressure variances in the headspace, therefore allowing detection of pressure transients due to metabolic processes where microbes are present.

Continuous data collected by the Speedy Breedy is analyzed in real time to provide pressure transients. Detection algorithms process these pressure transients to alert when significant changes have taken place.

Speedy Breedy measures both positive and negative pressure meaning that monitoring can be performed on a range of microbial processes reacting to differing conditions within the culture chamber.

Specification

Speedy Breedy is extremely compact, weighing just 3.0 kilos it's portable and operates on 12 volts dc and mains adapters.

- Weight of packaged unit (incl. Carry case, Power pack, 8 vessels, manual etc): 4.8kg
- Height: 13.3cm
- Width: 31cm
- Depth: 11.2cm

Prices

Product	Quantity	Price	Order
Speedy Breedy	1	5700 €
Culture Vessel - Unfilled (Sterilised)	10x	110 €
Culture Vessel - Filled (TSB) (Sterilised)	10x	150 €
Culture Vessel - Filled (McC) (Sterilised)	10x	150 €

From 8th to 31st of March GENTAUR’s ELISA kits of highest quality are with 30% discount!!!

Cat.	Product	Old Price	Promo Price	Order
CK-E94663	.Mouse aldosterone (ALD) ELISA Kit	615 €	430 €
CK-E90283	90283Human Soluble CD163_sCD163_ ELISA Kit	615 €	430 €
CK-E90006	ACTH ELISA Kit	615 €	430 €
CK-E90364	Adiponectin Receptor 1 ELISA Kit	615 €	430 €
CK-E94919	Aflatoxin albumin adduct Elisa kit	615 €	430 €
CK-E95548	Aflatoxin albumin adduct Elisa kit	615 €	430 €
CK-E90363	Aflatoxin albumin adduct Elisa kit	615 €	430 €
CK-E90365	Aflatoxin Elisa Test kit	615 €	430 €
CK-E90060	Aflatoxin,AFT ELISA Kit	615 €	430 €
CK-E90010	AMPK ELISA Kit	615 €	430 €

Click here to see more products from the promotion

The findings of a recent study indicate that routine testing with both fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC) may enhance the detection of ALK-positive non-small cell lung cancer (NSCLC). Accurate determination of ALK-positive tumors is necessary to identify patients with advanced NSCLC who are most likely to benefit from targeted therapy with an ALK inhibitor.

The discovery of ALK rearrangement in about 1% to 7% of NSCLCs led to the development of ALK inhibitors, such as crizotinib, which have significantly improved treatment response among people with ALK-positive NSCLC. FISH with break-apart probes is currently the only approved test for the detection of ALK positivity in NSCLC, but this technique may not be available or feasible in all institutions. IHC, which is more affordable and accessible than FISH, has been found to be reliable as a screening tool, but interpretation of its results have not been standardized. Researchers continue to explore the optimal testing process for identifying ALK-positive NSCLCs.

Parallel testing with both FISH and IHC on 3,234 NSCLCs led to the identification of 150 tumors that were ALK positive by either test. The results of FISH and IHC were discordant in 80 of these tumors. As such, the use of FISH or IHC alone would have missed approximately 25% of ALK-positive cases. The findings of the study are published in the March issue of the International Association for the Study of Lung Cancer's journal, the Journal of Thoracic Oncology (JTO).

Most discordant results were FISH-positive/IHC-negative (36 cases); FISH-negative/IHC-positive results were found in 19 cases, and FISH-noncontributive/IHC-positive results were found in 15 cases. Preliminary data from 44 evaluable patients showed that treatment with an ALK inhibitor was associated with a high response rate, with response noted among patients who had tumors with discordant results (either FISH-positive/IHC-negative or FISH-negative/IHC-positive).

"Data on crizotinib response in patients who have been diagnosed differently by FISH and IHC are still preliminary. Thus, until large-scale studies in patients under therapy with crizotinib determine which testing is the most relevant to predict responses to ALK inhibition, our data support the need to routinely perform both analyses because of the difficulty in detecting the chimeric ALK protein in NSCLC and the presence of false-negative cases for each method," says lead author Florian Cabillic, PharMD, PhD, Université de Rennes, Rennes, France.

The study also demonstrated that systematic testing of NSCLC by both FISH and IHC is feasible in routine practice. In addition, the findings indicate that performing FISH routinely on all NSCLC specimens may require an automated process at various stages of the analysis.

High quality aqueous mounting medium with very high refractive index, which can be used to store stained tissue specimens permanently without the fading of chromogen stains.

CC/Mount Advantages:

- No coverslip required
- No exposure to the organic fumes
- Ensures permanent storage of slides
- Compatible with wide range chromogens like AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins
- High pH ensures increased stability of fluorescence

Catalog No.	Product	Datasheet
K 002	CC/Mount Ready To Use 30 ml
K 002-200	CC/Mount Ready To Use 200 ml