Targatt offers a mouse embryonic stem cell (ESC) derivation service from any strain of your interest. We can also derive ESC lines from transgenic mice.

a) NCSCs forming characteristic “lacunae” during
proliferation on ASC’s NCSC media (10X)
b) NCSCs at a cell density ready for passaging.(20X)

Background: One of the defining characteristics of vertebrate development is the migration of neural crest cells from the neural tube. Neural crest cells give rise to a variety of tissues such as melanocytes, some heart vessels, cephalic neuroendocrine organs, connective tissue of the craniofacial skeleton and the neurons and glia of both the sensory and autonomic ganglia of the peripheral nervous systems. The study of neural stem cells and their progenitors, Neural Crest Stem Cells (NCSCs), have direct medical implications. Numerous pathologies, such as skeletal and nervous system disorders and peripheral neuropathies, arise from aberrant migration, specification or differentiation of neural crest cells. In order to assist in your neural crest cell studies, our NCSC media has been optimized to contain all the factors required to support NCSCs plated on fibronectin coated tissue culture surfaces.
Product description: Add 2 mL of NCSC medium supplement to 498 mL of NCSC medium to produce 500 mL of NCSC media.
Storage conditions for NCSC medium: -80°C; 1 year, 4°C; two weeks. Minimize exposure to direct light.
Storage condition for NCSC supplement: -80°C; 1 year, 4°C; two weeks.
Storage condition for NCSC media: -80°C; 1 year, 4°C; two weeks. Minimize exposure to direct light.
Shipping: On dry ice.
Recommended procedure:
1. Thaw NCSC medium either overnight at 4°C or for several hours at RT.
2. Thaw the NCSC medium supplement at RT.
3. Using sterile technique, add the NCSC medium supplement to the NSCS medium. Mix thoroughly and store the newly made media at 4°C for up to two weeks. NOTE: NCSC medium can be filter sterilized if desired. Do NOT filter sterilize the NCSC medium supplement. Add this supplement directly to the NCSC medium.
4. Prior to use, warm the media to RT in the tissue culture hood. Due to the complex composition of the NCSC media, we do not recommend warming the media in a 37 C water bath.

 Price: 80 EUR

TARGATT Transgenic Kit is designed to create site-specific, knock-in transgenic mice in a more efficient and significantly faster way over traditional methods. Generating transgenic mice by conventional methods (e.g. pronuclear microinjection or lentiviral injection) has several limitations, first of which is random insertion of the transgene. Random insertion of a transgene results in a position effect where either the transgene is prone to silencing or endogenous gene expression is disrupted. Furthermore, this position effect is compounded when a transgene is inserted as multiple copies, resulting in instability at the insertion locus.

Using our proprietary site-specific DNA integration system, TARGATT Transgenic Kit, combined with our genetically TARGATT mouse embryos (cat.# AST-0001, AST-0002, AST-0003, AST-0004). Targatt offers high quality H11(Hipp11) and ROSA26 transgenic mouse kits with our TARGATT technology. Using our TARGATT Technology, you can generate your own knock-in mouse in just three months.

TARGATT embryos are derived from one of our genetically engineered mouse models. These mouse embryos can be used as embryo donors for creating site-specific transgenic mice in a more efficient and faster way compared to traditional methods. Generating transgenic mice by conventional methods (e.g. pronuclear microinjection or lentiviral injection) has several limitations, first of which is the random insertion of the transgene. Random insertion of a transgene results in a position effect where either the transgene is prone to silencing or endogenous gene expression is disrupted. Furthermore, this position effect is compounded when transgenes are inserted in multiple copies, resulting in genomic instability at the insertion locus. Using our proprietary site-specific DNA integration technology, TARGATT embryos combined with our TARGATT Transgenic Kit (cat# AST-1001 or AST-1002), you can generate your desired transgenic mouse models with guaranteed gene expression faster.

Gentaur offers high quality H11 and ROSA26 TARGATT frozen embryos. Using our TARGATT Technology, you can generate your own knock-in mouse from H11 or ROSA26 embryos in 3 months. Our H11(Hipp11) and ROSA26 frozen embryos contain 3 straws with a total 45-60 cryopreserved embryos (8-cell stage/morula). They are shipped in a dry shipper containing liquid nitrogen. We also offer fresh H11 and ROSA26 embryos with each vial containing 25-35 fresh embryos (E3.5) in KSOM medium. We offer overnight shipping at ambient temperature. Embryos will be transferred to recipient(s) immediately upon arrival. US customers only.

TARGATT Embryos

Using our novel TARGATT system, a gene of interest can be specifically inserted at a well-characterized, transcriptionally-active locus in the mouse genome with guaranteed transgene expression. Tissue-specific and / or ubiquitous expression options are available.

Advantages of TARGATT technology:

1. Site-specific gene integration at a transcriptionally-active locus ensures high-level gene expression.
2. Integration happens at an intergenic region; no internal genes are disrupted.
3. The integrase system catalyzes a unidirectional integration event and results in a high efficiency in producing transgenic mice.
4. Gene integration at the same locus allows a precise comparison of the transgenics from one line to another.

TARGATT Kits

TARGATT Supporting Materials

Mouse Embryonic Fibroblasts (MEF)

●  CF-1
●  Neo-resistant
●  DR4
●  SNL (STO feeder cells)

SNL (STO feeder cells)

Cell Culture Products

●  Germline-tested & ESC-qualified FBS
●  Specialty Media
●  Basal Media (DMEM)
●  Stem Cell Growth and Differentiation Factors
●  ASC Small Molecules
●  3D Culture and Expansion System

Reprogramming

ESC/iPSC Characterization

  Pluripotency Protein Markers                   Stem Cell Gene Array
     

●  Pluripotency mRNA Markers
●  Components

ESC/iPSC Differentiation

●  Neural Differentiation
●  Dendritic Cell (DC) Generation

ES/iPS Cell Lines

Mouse ES Cell Lines                                 Human iPS Cells

Cell Depository

Primary Cell, cDNA, RNA (Normal)

●  Endocrine
●  Neural
●  Pulmonary
●  Digestive
●  Urinary
●  Reproductive
●  Skeletal Muscle
●  Hematopoietic
●  Integumentary
●  Cardiovascular
●  Miscellaneous

Primary Cell, cDNA, RNA (Disease)

●  Blood Disorders
●  Neurological Disorders
●  Degenerative Disorders
●  Metabolic Disorders
●  Cardiovascular Disorders
●  Congenital Disorders
●  Endocrine Disorders
●  Autoimmune Disorders
●  Genetic Disorders
●  Muscular Disorders
●  Oncogenic Disorders

All products - Full view result 

Read more about Targatt gene modification here