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GENTAUR Europe BVBA Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45 Fax 0032 16 50 90 45 This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it. |
GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280
Fax 0035929830072
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GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50
Fax 01 43 25 01 60
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GmbH Marienbongard 20
52062 Aachen Deutschland
Tel (+49) 0241 56 00 99 68
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GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531
Fax 020 8445 9411
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GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
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GENTAUR Nederland BV
Kuiper 1
5521 DG Eersel Nederland
Tel 0208-080893
Fax 0497-517897
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GENTAUR SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93
Fax 02 36 00 65 94
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GENTAUR Spain
Tel 0911876558
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Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
Phone/Fax:
(408) 780-0908
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GENPRICE Inc. invoicing/ accounting:
6017 Snell Ave, Suite 357
San Jose, CA. 96123
Serbia, Macedonia,
Montenegro, Croatia:
Tel 0035929830070
Fax 0035929830072
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GENTAUR Romania
Tel 0035929830070
Fax 0035929830072
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GENTAUR Greece
Tel 00302111768494
Fax 0032 16 50 90 45
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Other countries
Luxembourg +35220880274
Schweiz Züri +41435006251
Danmark +4569918806
Österreich +43720880899
Ceská republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Magyarország Budapest +3619980547
Lipofector 2000 and Lipofector EZ
For broad cell lines, lipofector-2000 or Lipofector-EZ Reagent delivers
DNA or siRNA with excellent transfection performance.
Description
- - High transfection efficiency in a broad spectrum of commonly transfected cell lines
- High performance for the delivery of plasmid DNA, siRNA, antisense oligo and miRNA
- Highest quality assured : endotoxin & sterility tests are passed.
Transfection Efficiency
- Cell Line : 293T
- DNA : pcDNA-GFP 0.2 ug/well
- Cell Line : MCF7
- DNA : pcDNA-GFP 0.4 ug/well
- Cell Line : 293T
- RNA : 5'cap-Fluc RNA reporter 0.2 ug/well
Key Features of Lipofector 2000 and Lipofector EZ
- • Excellent transfection efficiency
- Achieves high efficiency and expression results for plasmid or RNAi
transfections
• Wide range of successfully transfected cell lines
- Works effectively with many cell types (adherent or suspension)
• Convenient protocol
- Highly efficient transfection ratio in serum containing medium.
• Cost effective products
Product name | Cat.No. | Product size | Price |
Lipofector-2000 | AB-LF-2001 | 0.75 ml | 316€ |
AB-LF-2002 | 1.5 ml | 461€ | |
Lipofector-EZ | AB-LF-EZ075 | 0.75 ml | 318€ |
AB-LF-EZ100 | 1.0 ml | 364€ | |
AB-LF-EZ150 | 1.5 ml | 463€ | |
Lipofector-2000/EZ Combo | AB-LF-C075 | 0.75 ml + 0.75 ml | 469€ |
More: Lipofectors
Herc1-set siRNA/shRNA/RNAi Lentivector
Species | Mouse |
Accession Number | NM_145617.3 |
Vector | piLenti-siRNA-GFP |
Target Sequence | Sequence available upon placing order |
Sequence Primers | Sequence available upon placing order |
User Manual | |
Bacterial Selection | Kanamycin |
Mammalian Selection | Puromycin |
Format | Plasmid |
Appearance | Liquid |
Storage | -20°C or below |
Shelf Life | 1 year (when at -20°C or below in a non-frost free freezer) |
Shipping | Shipped at ambient temperature |
Quality Control | Restriction Enzyme Digest and Sequencing |
Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
More Information |
Our RNA interference lentiviral vectors contain siRNAs. We employ a dual convergent promoter system where the sense and antisense strands of the siRNA are expressed by two different promoters rather than in a hairpin loop - to avoid any possible recombination events that can occur. |
AccuTarget™ Custom Designed siRNA Synthesis
Gene silencing by RNA interference (RNAi) technology using small interfering RNA (siRNA) is a powerful tool for studying functional genomics, drug target identification and validation, pathway elucidation, and therapeutic development. The advantage is in the algorithm we use for siRNA design. Our Turbo si-Designer software identifies highly effective siRNA target sites with remarkably high siRNA knockdown rates. Critical parameters including base composition, thermodynamic instability and base preference are all considered in the design algorithm. siRNAs spanning SNP sites are removed and finally, non-specific siRNAs are eliminated following homology searching by BLAST and Smith-Waterman algorithms. The result is an siRNA with extraordinary siRNA knockdown efficiency and minimal off-target effects. The performance of Turbo si-Designer has been extensively evaluated by testing the siRNA knockdown efficiency of thousands of siRNAs targeting STE Kinases, TK Kinases, genes involved in the NF-kappaB and Caspase Pathways using Real-time PCR analysis. The results of the evaluation indicated the design algorithm was highly effective in selecting effective siRNAs; 80% of the tested siRNAs showed > 70% knockdown and 38% elicited knockdown of > 90%.
Cat. No. | Guaranteed Yield | Purification |
S-1017-5 | 1 nmole | BioRP |
S-1017-6 | 5 nmole | |
S-1017-1 | 10 nmole | |
S-1017-2 | 20 nmole | |
S-1017-3 | 50 nmole | |
S-1017-4 | 100 nmole | |
S-1018-5 | 1 nmole | HPLC |
S-1018-6 | 5 nmole | |
S-1018-1 | 10 nmole | |
S-1018-2 | 20 nmole | |
S-1018-3 | 50 nmole | |
S-1018-4 | 100 nmole |
- siRNA provided lyophilized and annealed.
- Annealing buffer is provided when single-stranded siRNA requested.
AccuTarget Control siRNAs
AccuTarget™ Positive Control siRNAs
Cat. No. | Product Description | Purification | Guaranteed Yield |
SP-1001 | AccuTarget™ GAPDH Positive Control siRNA | BioRP | 5 nmole |
SP-1002 | AccuTarget™ GAPDH Positive Control siRNA | BioRP | 10 nmole |
SP-1003 | AccuTarget™ GAPDH Positive Control siRNA | BioRP | 20 nmole |
SP-1011 | AccuTarget™ GAPDH Positive Control siRNA | HPLC | 5 nmole |
SP-1012 | AccuTarget™ GAPDH Positive Control siRNA | HPLC | 10 nmole |
SP-1013 | AccuTarget™ GAPDH Positive Control siRNA | HPLC | 20 nmole |
SP-2001 | AccuTarget™ GFP Positive Control siRNA | BioRP | 5 nmole |
SP-2002 | AccuTarget™ GFP Positive Control siRNA | BioRP | 10 nmole |
SP-2003 | AccuTarget™ GFP Positive Control siRNA | BioRP | 20 nmole |
SP-2011 | AccuTarget™ GFP Positive Control siRNA | HPLC | 5 nmole |
SP-2012 | AccuTarget™ GFP Positive Control siRNA | HPLC | 10 nmole |
SP-2013 | AccuTarget™ GFP Positive Control siRNA | HPLC | 20 nmole |
SP-3001 | AccuTarget™ Luciferase Positive Control siRNA | BioRP | 5 nmole |
SP-3002 | AccuTarget™ Luciferase Positive Control siRNA | BioRP | 10 nmole |
SP-3003 | AccuTarget™ Luciferase Positive Control siRNA | BioRP | 20 nmole |
SP-3011 | AccuTarget™ Luciferase Positive Control siRNA | HPLC | 5 nmole |
SP-3012 | AccuTarget™ Luciferase Positive Control siRNA | HPLC | 10 nmole |
SP-3013 | AccuTarget™ Luciferase Positive Control siRNA | HPLC | 20 nmole |
AccuTarget™ Negative Control siRNAs
Cat. No. | Product Description | Purification | Guaranteed Yield |
SN-1001 | AccuTarget™ Negative Contol siRNA | BioRP | 5 nmole |
SN-1002 | AccuTarget™ Negative Contol siRNA | BioRP | 10 nmole |
SN-1003 | AccuTarget™ Negative Contol siRNA | BioRP | 20 nmole |
SN-1011 | AccuTarget™ Negative Contol siRNA | HPLC | 5 nmole |
SN-1012 | AccuTarget™ Negative Contol siRNA | HPLC | 10 nmole |
SN-1013 | AccuTarget™ Negative Contol siRNA | HPLC | 20 nmole |
SN-1021 | AccuTarget™ Fluorescein-labeled Negative Control siRNA | HPLC | 5 nmole |
SN-1022 | AccuTarget™ Fluorescein-labeled Negative Control siRNA | HPLC | 10 nmole |
SN-1023 | AccuTarget™ Fluorescein-labeled Negative Control siRNA | HPLC | 20 nmole |
AccuTarget™ Control siRNA Sets
Cat. No. | Product Description | Purification | Guaranteed Yield |
SS-1001 | AccuTarget™ GAPDH Control siRNA Set | BioRP | 5 nmole positive control + 2 nmole negative control |
SS-1002 | AccuTarget™ GFP Control siRNA Set | BioRP | 5 nmole positive control + 2 nmole negative control |
SS-1003 | AccuTarget™ Luciferase Control siRNA Set | BioRP | 5 nmole positive control + 2 nmole negative control |
SS-1011 | AccuTarget™ GAPDH Control siRNA Set | HPLC | 5 nmole positive control + 2 nmole negative control |
SS-1012 | AccuTarget™ GFP Control siRNA Set | HPLC | 5 nmole positive control + 2 nmole negative control |
SS-1013 | AccuTarget™ Luciferase Control siRNA Set | HPLC | 5 nmole positive control + 2 nmole negative control |
AccuTarget™ Premade siRNA Sets
siRNA Set
Cat. No. | Product Description | No. of Genes | No. of siRNAs |
SHS-0010 | AccuTarget™ Human Antioxidant siRNA Set | 38 | 114 |
SHS-0020 | AccuTarget™ Human Apoptosis siRNA Set | 290 | 870 |
SHS-0250 | AccuTarget™ Human Cancer siRNA set | 1157 | 3471 |
SHS-0030 | AccuTarget™ Human Caspase siRNA set | 37 | 111 |
SHS-0040 | AccuTarget™ Human Cell cycle siRNA Set | 112 | 336 |
SHS-0050 | AccuTarget™ Human Cyclase siRNA Set | 21 | 63 |
SHS-0060 | AccuTarget™ Human Cytochrome P450 siRNA Set | 52 | 156 |
SHS-0070 | AccuTarget™ Human Deaminase siRNA Set | 22 | 66 |
SHS-0080 | AccuTarget™ Human GPCR signaling pathway siRNA set | 727 | 2181 |
SHS-0090 | AccuTarget™ Human Helicase siRNA Set | 114 | 342 |
SHS-0100 | AccuTarget™ Human Isomerase siRNA Set | 104 | 312 |
SHS-0110 | AccuTarget™ Human Kinase siRNA Set | 699 | 2097 |
SHS-0120 | AccuTarget™ Human Ligase siRNA Set | 272 | 816 |
SHS-0130 | AccuTarget™ Human Lyase siRNA Set | 123 | 369 |
SHS-0140 | AccuTarget™ Human Motor siRNA Set | 122 | 366 |
SHS-0150 | AccuTarget™ Human NF-kB pathway siRNA Set | 37 | 111 |
SHS-0160 | AccuTarget™ Human Nucleic acid binding siRNA Set | 2573 | 7719 |
SHS-0170 | AccuTarget™ Human Oxidoreductase siRNA Set | 551 | 1653 |
SHS-0180 | AccuTarget™ Human Peptidase siRNA Set | 491 | 1473 |
SHS-0190 | AccuTarget™ Human Phosphatase siRNA Set | 188 | 564 |
SHS-0200 | AccuTarget™ Human Receptor siRNA Set | 1516 | 4548 |
SHS-0210 | AccuTarget™ Human Transferase siRNA Set | 1428 | 4284 |
SHS-0220 | AccuTarget™ Human Transporter siRNA Set | 1021 | 3063 |
SHS-0230 | AccuTarget™ Human Tubulin siRNA Set | 20 | 60 |
SHS-0240 | AccuTarget™ Human Ubiquitin siRNA Set | 77 | 231 |
siRNA Subset
Cat. No. | Product Description | No. of Genes | No. of siRNAs |
SHS-0110-1 | AccuTarget™ Human AGC Kinase siRNA Subset | 58 | 174 |
SHS-0110-2 | AccuTarget™ Human CAMK Kinase siRNA Subset | 65 | 195 |
SHS-0110-3 | AccuTarget™ Human CK Kinase siRNA Subset | 11 | 33 |
SHS-0110-4 | AccuTarget™ Human CMGC Kinase siRNA Subset | 56 | 168 |
SHS-0110-5 | AccuTarget™ Human Other Kinase siRNA Subset | 342 | 1026 |
SHS-0110-6 | AccuTarget™ Human STE Kinase siRNA Subset | 43 | 129 |
SHS-0110-7 | AccuTarget™ Human TK Kinase siRNA Subset | 84 | 252 |
SHS-0110-8 | AccuTarget™ Human TKL Kinase siRNA Subset | 40 | 120 |
SHS-0190-1 | AccuTarget™ Human Other Phosphatase siRNA Subset | 147 | 441 |
SHS-0190-2 | AccuTarget™ Human Tyrosine Phosphatase siRNA Subset | 41 | 123 |
siRNA Druggable Set
Cat. No. | Product Description | No. of Genes | No. of siRNAs |
SHD-00XX | AccuTarget™ Human Druggable siRNA library set | 8175 | 24,525 |