Studies of "drug" are still in a very early stage and tests are performed only on mice.

Soon fans will be able to cup sober just one pill. Researchers made ​​a cocktail of alcohol metabolizing enzymes rapidly reduce the level of alcohol in the blood while drunk mouse forward "Daily Mail". 

"Treatment", which compares with having millions of liver cells in your stomach, you may have a lasting impact on fans of spirits. Yangfeng Lou - professor of chemistry and biomolecular engineering, and Cheng Zhi - Professor of Biochemistry and Molecular Biology at the University of Southern California, injected mice with drunk nanocapsule containing two enzymes. One of the enzymes - oxidase comes as a by-product of hydrogen peroxide, which can be harmful. That's why he has to work with another enzyme to break down hydrogen peroxide. The study shows that drunken mice that were injected with these nanocapsule, sobered much faster than those who did not receive enzyme "treatment". The breakthrough is still in a very early stage and can not speak for its application in humans.

But expert Lou argues that experience can lead to the invention of a new drug to act as an antidote to alcohol. He states that "drug" can be taken as a simple pill. "It's like you have millions of liver cells in your stomach that help you revise alcohol," said Professor Lu.

Scientists develop pill that can help people to live to 150 years, slowing the aging process, reported the British newspaper "Daily Telegraph".

Drugs are synthetic versions of the organic chemical resveratrol, found in red wine, which is believed to slow aging, enhances the activity of the protein SIRT1. The pharmaceutical company GlaxoSmithKline (GSK) drug testing on people suffering from diabetes of the second type and psoriasis.

Professor of Genetics at Harvard University David Sinclair said that aging can not "irreversible catastrophe." "We now investigate whether a benefit for people who are already healthy. Results are promising. We find that aging is irreversible disaster, as previously thought. Certain people may live up to 150 years, but it will not get there without further research" he said.

Professor Sinclair said that the efforts of the activity of SIRT1 protein enhances cell activity, reducing their laziness. Previous experiments on mice, bees and flies that received the substances that enhance the activity of the protein showed that they live longer.

Professor Sinclair allegedly performed experiments which showed that the substances based on resveratrol have a direct impact on health. Some scholars argue that the impact is real and experimental stealth. The results were published in the scientific journal "Science", wrote Thursday.

Despite the controversy, some experiments that promise for diseases such as cancer, cardiovascular disease and heart failure, diabetes of the second type, Alzheimer's, Parkinson's, fatty liver disease, cataracts, osteoporosis, muscle atrophy, sleep disorders and inflammatory diseases such as psoriasis, arthritis and colitis.

In the present study aimed to determine how these substances can help cure diseases associated with aging. But Professor Sinclair believes that in time will also examine the preventive effect. As statins are used today to prevent heart disease and stroke, as these substances can be used to delay many diseases, says the researcher.

We provide a high quality service for the generation of gene-targeted knock-in and knock-out mouse models. Our team has decades of experience in vector design, ES cell targeting and mouse handling. We will discuss your project needs with you and custom design your knockin/knockout mouse model. Our service includes consultation on available gene targeting vector design strategies and project assessment based on your model requirements.

Service Milestones for the Generation of Knock-in/Knock-out Mouse Models

• - Gene targeting vector construction and sequencing
• - ES cell targeting, screening and expansion of positive ES cell clones
• - Karyotyping of targeted ES cells
• - Cre/FLP recombination (optional)
• - Chimera production by blastocyst injection
• - Breeding of chimeras for germline transmission
• - Genotyping of offspring
• - Transfer of heterozygous targeted mice to the customer
• - Optional: breeding to generate homozygous mice

Features

Cost-effective

• - Targatt has years of experience and an exceptionally high success rate in generating conventional gene-targeted mice with our proprietary mESC lines.

High-Quality

• - Our scientists have extensive experience in gene targeting and genetic mouse models. Our services always include a rigorous quality control with multiple check-points to ensure your projects are completed with highest accuracy. All mice are generated in and shipped from our California facility (NIH guidance certified).

Please contact us with any questions regarding services not listed. We are happy to discuss your project with you and design customized strategies. We also offer Transgenic Mouse (Random Insertion) and Transgenic Rat Services (knockin, knockout).

Testimony of Dr. Zhenheng Guo, PhD, Assistant Professor of Internal Medicine, Division of Endocrinology and Molecular Medicine, University of Kentucky

Project: Germline-transmitted, conditional knock-out mice by tetraploid complementation

"Overall, I am very satisfied with the quality of your service. After we received the mice, we did extensive studies to characterize them. All of the PCR reactions confirm the gene modifications are in the correct sites. We have also crossed mice with Cre mice to demonstrate that the Cre deleted the exon in heterozygous pups as expected. All data obtained showed that the mice were correctly targeted. In addition, I am very happy with the frequent communication with your scientists during the process. I highly recommend your services."

Project types

Knock-in Mouse Models

The introduction of a gene into a specified location of the mouse genome can be used for various applications. Mice can be homozygous or heterozygous for the inserted gene.

• - Reporter genes (e.g. GFP, lacZ) are used for expression analysis of a gene of interest. The reporter gene is inserted into the gene of interested in-frame (non-disruptive), allowing for visualization of temporal and spatial gene expression pattern.
• - Humanized disease models can be generated by inserting a human mutant gene or gene fragment into the corresponding mouse gene. The diseased human allel is thus transcribed in the appropriate genomic context and can be analyzed on behavioral, pathological, cellular and/or molecular level.
• - DNA recombinases (CRE, FLP) can be inserted into a gene of interest. The celltype-specific expression of the recombinase then allows for gene inactivation in the desired tissue after crossing this knock-in mouse with a conditional knockout mouse.

Knock-out Mouse Models

The knockout technology is most commonly used for gene inactivation, either in a constitutive or conditional fashion.

• - Constitutive knock-out mouse models are widely used to study gene function. The gene of interest is permanently inactivated in all cells of the animal. However, this non-conditional knock-out may cause lethality and is therefore not always recommended.
• - Conditional knock-out models are inducible - the targeted gene is excised after crossing the mouse with a Cre-transgenic mouse line. Gene inactivation can be celltype-specific and/or chemically induced.

Targatt can create site-specific knock-in mice for you within 3 months. Using our novel TARGATT system, a gene of interest can be inserted at a well-characterized, transcriptionally-active locus in the mouse genome with guaranteed transgene expression. Tissue-specific and/or ubiquitous expression options are available. Please contact us for a list of plasmid construct with reporter genes. 

In addition to our full service we also offer individual service options to generate your TARGATT knock-in mouse.

You can prepare the DNA using the TARGATT Kit and we do the pronuclear microinjection for you. Or we can prepare the DNA that you then inject after setting up your own TARGATT mouse colony.

Please contact us to discuss your project plan for your fast & site-specific knock-in or TARGATT Knock-down Mouse services.

TARGATT Embryos

Using our novel TARGATT system, a gene of interest can be specifically inserted at a well-characterized, transcriptionally-active locus in the mouse genome with guaranteed transgene expression. Tissue-specific and / or ubiquitous expression options are available.

Advantages of TARGATT technology:

1. Site-specific gene integration at a transcriptionally-active locus ensures high-level gene expression.
2. Integration happens at an intergenic region; no internal genes are disrupted.
3. The integrase system catalyzes a unidirectional integration event and results in a high efficiency in producing transgenic mice.
4. Gene integration at the same locus allows a precise comparison of the transgenics from one line to another.

TARGATT Kits

TARGATT Supporting Materials

Mouse Embryonic Fibroblasts (MEF)

●  CF-1
●  Neo-resistant
●  DR4
●  SNL (STO feeder cells)

SNL (STO feeder cells)

Cell Culture Products

●  Germline-tested & ESC-qualified FBS
●  Specialty Media
●  Basal Media (DMEM)
●  Stem Cell Growth and Differentiation Factors
●  ASC Small Molecules
●  3D Culture and Expansion System

Reprogramming

ESC/iPSC Characterization

  Pluripotency Protein Markers                   Stem Cell Gene Array
     

●  Pluripotency mRNA Markers
●  Components

ESC/iPSC Differentiation

●  Neural Differentiation
●  Dendritic Cell (DC) Generation

ES/iPS Cell Lines

Mouse ES Cell Lines                                 Human iPS Cells

Cell Depository

Primary Cell, cDNA, RNA (Normal)

●  Endocrine
●  Neural
●  Pulmonary
●  Digestive
●  Urinary
●  Reproductive
●  Skeletal Muscle
●  Hematopoietic
●  Integumentary
●  Cardiovascular
●  Miscellaneous

Primary Cell, cDNA, RNA (Disease)

●  Blood Disorders
●  Neurological Disorders
●  Degenerative Disorders
●  Metabolic Disorders
●  Cardiovascular Disorders
●  Congenital Disorders
●  Endocrine Disorders
●  Autoimmune Disorders
●  Genetic Disorders
●  Muscular Disorders
●  Oncogenic Disorders

All products - Full view result 

Read more about Targatt gene modification here

A drug currently approved to treat mouth ulcers has shown promise in animal studies for being a contender in pharmaceutical weight loss. Amlexanox was found by University of Michigan researchers to produce weight loss in obese mice without any change in diet or exercise habits.

For the study, mice fed a high calorie diet until they became obese were injected with amlexanox. The animals lost weight, despite consuming the same amount of calories. The researchers also noted a loss in overall body fat, a decrease in fatty liver, and a reversal of obesity-induced type 2 diabetes. Once taken off the amlexanox injections, however, the mice experienced weight gain.

Amlexanox may work by changing the action of genes that control metabolism versus working as an appetite suppressant.

When the drug was injected in mice, the drug worked by increasing metabolism, not by suppressing appetite.

"One of the reasons that diets are so ineffective in producing weight loss for some people is that their bodies adjust to the reduced calories by also reducing their metabolism, so that they are 'defending' their body weight," says Dr. Alan Saltiel, the lead researcher at the University of Michigan.

 "Amlexanox seems to tweak the metabolic response to excessive calorie storage in mice."

The findings were published Sunday in the journal Nature Medicine. Clinical trials are expected to begin later this year to test the drug's effectiveness in humans.

TARGATT Fast & Efficient Gene Modification in Human Cells. Make your cell line in 3 months!

• - Efficient insertion of any gene
• - Single-copy
• - Stable expression
•  
•  Click here to see all products
•  

Based on our proprietary site-specific TARGATT technology for fast knock-in mouse (KI) generation, Applied StemCell Inc. (ASC) recently developed a TARGATT system specifically for introducing gene(s) of interest into mammalian cell lines. The unique advantage of this system is that any gene of interest can be inserted efficiently into a defined, transcriptionally-active locus with high gene expression in a single copy fashion.

Technical Details:

TARGATT technology enables highly efficient site-specific gene integration in mammalian cells and animals1, 2. This technology uses ?C31 integrase to insert any gene of interest into a docking site, pre-engineered in an intergenic region and transcriptionally active genomic locus. Our TARGATT technology improves several aspects in the generation of transgenic cell lines and animals: (1) High integration efficiency mediated by ?C31 integrase reduces time and cost; (2) Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high level expression of the transgene; (3) Integration at intergenic region ensures that no internal genes are interrupted; (4) Single copy gene integration eliminates repeat-induced gene silencing and genomic instability; (5) Site-specific integration allows a precise comparison of the effects of the transgenes among different lines. TARGATT technology can be utilized for a variety of applications including reporter gene expression, gene knockdown, disease cell and animal models.

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