- Removes >90% albumin from 30 mg albumin/ml sample

- Affinity-type equivalence, virtually no cross-reactivity with other proteins

- Bind and elute procedure - simply weigh powder, condition the sample, centrifuge and/or filter, and recover the albumin depleted serum

- Economical new surface technology, not based on affinity chromatography

- Mild conditions maintain tertiary structure of proteins and simple transfer to secondary analysis

- The albumin depleted filtrate retains the enzymatic and biological activity

- Removes albumin from samples such as serum, plasma and from species including human, mouse, sheep, bovine, goat, rat, and calf

- The flow through fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.

Albusorb™ is an albumin depletion reagent supplied as a kit with necessary buffers. With 25ul of starting sample, the yield of albumin depleted serum protein is 0.1-0.2 milligrams. Albusorb™ binds albumin from serum or plasma and is compatible with downstream proteomics methods such as protein array pixelation ,1D and 2D gel electrophoresis, LC/MS, and MALDI-TOF MS. Samples are also prepared for biomarker discovery, toxicological studies for new drugs, enzyme assays, protein profiling using SELDI analysis and cytokines research.

AlbuSorb™ binds to albumin, and serum proteins flow through. Removal of albumin allows enhanced detection of low abundance proteins. AlbuSorb™ comes from a silica-based, separation platform utilizing a new combination of surface microenvironments substituted with low molecular substrates that feature drug-binding motifs. Unlike immuno-affinity, the surfaces utilized are disposable eliminating cycle to cycle variance and cross-contamination.

Download datasheet: AlbuSorb™ Albumin Depletion Kit 

Product list

Cat.	Product	Quantity	Promo Price	Order
A185-6	AlbuSorb™ , Albumin Depletion Kit	6 grams	460 €
A185-18	AlbuSorb™ , Albumin Depletion Kit	18 grams	1006 €
A185-3	AlbuVoid™ , Albumin Depletion and Low Abundance Protein Enrichment Kit	3 grams	505 €

More: Kits

TotalLab Quant is a suite of core analysis tools for quantitative image analysis applications in the Life Sciences.

New for v12 

   

TotalLab Quant contains modules for:

• 1D electrophoresis gel and Western blot analysis
• Array / dot blot / slot blot analysis
• Colony counting & basic 2D spot measurement
• General feature-based image analysis

A wide range of image formats can be analysed, maximising the image capture instruments you already have in your lab.

1D DNA gel	1D Protein gel	Western Blot	Protein Array	Colony Counting

Workflows to suit your analysis requirements

Analysis of such images is rapid, automated to a high level and reproducible.

The user has the ability to review each stage of the workflow analysis and intervene / edit if required. Combining high levels of automation with final user review allows rapid and accurate quantitative analysis.

The user then has full control of the visualisation tools and data display - outputting only those data fields that are of importance as well as the images of choice.

  

Fast Accurate Quantitation and Reporting

For 1D gels, highly developed algorithms accurately detect lanes and bands even on distorted gel images. Results can be verified using the range of visualisation tools which aid further examination of lane and band data.

Calibrate the bands using one or more Molecular Size standard lanes and derive accurate quantitation from known band volumes.

For the Arrays and Colony plates, rapid identification and quantitative measurements of all relevant features allow for speedy analysis and accurate data.

Order it NOW and get 10% discount

Price: 956 860 Euro

37.5:1 Acrylamide to Bisacrylamide Stabilized Solution

Optimized for SDS-PAGE of Proteins (Laemmli gels)
Consistently Crystal Clear Gels, Zero Fluorescence
Stabilized for Long Shelf Life

Catalog Number: EC-890
ProtoGel forms an electrophoresis matrix that is ideal for the separation of proteins and polypeptides. ProtoGel is a stabilized, ready-to-use 30% (w/v) acrylamide/methylene bisacrylamide solution (37.5:1 ratio), manufactured from the highest quality materials, from which virtually all impurities have been removed. ProtoGel has zero acrylic acid content, eliminating the fixed charges that cause band streaking. Additionally, oxidation products such as aldehydes have been removed by a selective adsorption process. Aldehydes can attack proteins, altering the structure of the sample, and thus altering Rf values. With ProtoGel, you can trust that your results will be consistent one electrophoretic run to the next.

Storage: ProtoGel is stable for 24 months when stored tightly capped in a dark area at room temperature (20°C).

ProtoGel (30%) Protocol
Measure and Mix Solutions:
The volume of ProtoGel required for gel casting solutions of any volume and acrylamide concentration may be calculated from the following formula:
Vp =(X) (Vt)/30
where:
Vp = Volume of 30% ProtoGel
X = % Monomer Desired in Gel
Vt = Total Volume of Gel Casting Solution

EXAMPLE: To make 100 ml of a 10% monomer gel, calculate the volume of Protogel to add as follows:
Vp = (10) (100)/30 = 33.3 ml

Initiate and Cast Gel:
For optimal results degas gel solution for 10 minutes under vacuum aspiration prior to innitiation with APS and TEMED. Add 1.0ml of 10% (w/v) ammonium persulfate for every 100ml of gel casting solution. Swirl gently to mix. Add 0.1 ml of TEMED for every 100ml of gel casting solution. Swirl gently to mix. Pour the solution into the gel casting cassette. The gel should begin to set in 10-20 minutes. To provide a sharp interface, overlay the gel with water saturated n-butanol during polymerization. Flush butanol away with water just before casting the stacking gel (below).

Cast Stacking Gel:
Use ProtoGel Stacking Buffer to make 10ml of a 4% stacking gel:
ProtoGel: 1.3ml
ProtoGel Stacking Buffer: 2.5ml
Deionized Water: 6.1ml
Add 0.05ml 10% Ammonium Persulfate and 0.01ml of TEMED. Gel will begin to set in 20 minutes.

Download datasheet

Price: 109 Euro

or Click Here