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GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
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    GENTAUR France

     GENTAUR France SARL
    9, rue Lagrange, 75005 Paris 
    Tel 01 43 25 01 50 
    Fax 01 43 25 01 60
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    Gentaur Germany

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      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
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    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
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    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
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    GENTAUR Nederland

     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
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    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
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    GENTAUR Spain

     GENTAUR Spain
    Tel 0911876558
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    Genprice USA

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    547, Yurok Circle
    San Jose, CA 95123
    Phone/Fax: 

    (408) 780-0908 

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    GENPRICE Inc. invoicing/ accounting:
    6017 Snell Ave, Suite 357
    San Jose, CA. 96123

     

    Gentaur Serbia

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    Tel 0035929830070 
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    Tel 0035929830070 
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    GENTAUR Greece

    grGENTAUR Greece 

    Tel 00302111768494 
    Fax 0032 16 50 90 45

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    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
    Danmark +4569918806
    Österreich +43720880899
    Ceská republika Praha +420246019719
    Ireland Dublin +35316526556
    Norge Oslo +4721031366
    Finland Helsset +358942419041
    Sverige Stockholm +46852503438
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    Dr. Ivan Campeotto

    MRC Centre for Molecular Bacteriology and Infection
    Imperial College London
    United Kingdom

    In my current project at Imperial College London, I am focusing on several proteins from different human pathogens. One of these proteins forms crystals, which tend to be looped with the unique c axis of ~500Å in length and oriented in parallel with the X–ray beam. This led to a severe spot–overlapping problem, which was resolved with the aid of a kappa goniometer.
    Gentaur products came to the rescue in different steps of this project.
    The first issue to solve was the fact that crystals grew on layers of microcrystals mixed with protein skin, which, when disturbed with a traditional loop, would break into many pieces, enter the loop and thereby abolish diffraction. The Microloops™ allowed for precise selection of the desired crystals without perturbation of the drop, so that skin-free crystals inside the loop could be obtained.

    targeting-rosa26-feeder-cells-human-primary-cells-mef-1

    Figure 1. Micro–crystals were used for in situ at Diamond beamline i04–1. X–ray beam and ruler are represented in red.Another problem was the reproducibility of the crystals, when crystallization trials were set–up in 96, 48 or 24 well plate formats.  I decided to try the new in–situ plates and to compare them with traditional 96 well, 48 well and 24 well plates. This experiment was performed on the same day and using the same protein batch. Whilst the other plates gave either only micro–crystals (<1μm) or failed completely to produce crystals within a given crystallization condition range, the Gentaur plates gave crystals of about 30μm in size (Fig.1)!

    targeting-rosa26-feeder-cells-human-primary-cells-mef-2

    Figure 2. In situ diffraction at the Diamond Light Source synchrotron from Gentaur plate (beam line i04–1).In addition the Gentaur in situ plate was easily transported to the synchrotron (Diamond, beamline i04–1), as it could be packed vertically. Diffraction experiments were performed in situ and crystals diffracted approximately to 3.8Å (Fig.2) with a very low background diffraction compared to competitors.
    In my opinion the greatest benefits were: crystal reproducibility, crystal–friendly plate transport and low background scattering. There are a few others benefits, which are not less important, such as the possibility of shooting the crystals with better orientations for data collection or the possibility of testing lattice disorders from data collected at RT vs. cryo data. These are all applications, which I am currently exploring at the i24 beamline at the Diamond Light Source (Oxford, UK), where complete data collection is currently achievable in situ.
    We all know that obtaining crystals and manipulating crystals represent a continuous challenge; therefore I would like to thank Gentaur for producing innovative products, which assist us crystallographers not only in delivering better results but also in overcoming experimental obstacles, which otherwise could severely delay or hamper our work.

    Gentaur’s In Situ 1 crystallization plates

    targeting-rosa26-feeder-cells-human-primary-cells-mef-3 targeting-rosa26-feeder-cells-human-primary-cells-mef-4

    Published in Tech Tips