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GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
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    GENTAUR France

     GENTAUR France SARL
    9, rue Lagrange, 75005 Paris 
    Tel 01 43 25 01 50 
    Fax 01 43 25 01 60
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    Gentaur Germany

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      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
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    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
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    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
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    GENTAUR Nederland

     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
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    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
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    GENTAUR Spain

     GENTAUR Spain
    Tel 0911876558
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    Genprice USA

    usa-flagGenprice Inc, Logistics
    547, Yurok Circle
    San Jose, CA 95123
    Phone/Fax: 

    (408) 780-0908 

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    GENPRICE Inc. invoicing/ accounting:
    6017 Snell Ave, Suite 357
    San Jose, CA. 96123

     

    Gentaur Serbia

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    montenegro-flagMontenegro, croatiaCroatia: 
    Tel 0035929830070 
    Fax 0035929830072
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    GENTAUR Romania

    romGENTAUR Romania

    Tel 0035929830070 
    Fax 0035929830072
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    GENTAUR Greece

    grGENTAUR Greece 

    Tel 00302111768494 
    Fax 0032 16 50 90 45

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    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
    Danmark +4569918806
    Österreich +43720880899
    Ceská republika Praha +420246019719
    Ireland Dublin +35316526556
    Norge Oslo +4721031366
    Finland Helsset +358942419041
    Sverige Stockholm +46852503438
    Magyarország Budapest +3619980547

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    Background staining or non-specific staining is an often-encountered problem in immunohistochemistry, in immunofluorescence and in situ stains. Background staining is caused by a number of factors such as cross reactivity of antibodies with the shared epitopes in the tissue, by the presence of natural and/or contaminating antibodies present in the primary antibody and/or the secondary antibody, by ionic interactions, by the presence of carbohydrates and by endogenous biotin present in the tissue. Eradicating background is most important for obtaining background-free specific staining for the ease of qualitative and quantitative evaluation.

    PRODUCT DESCRIPTION
    Background Buster is a peptide Blocker that eradicates all general background staining. Background Buster removes all background staining caused by primary antibodies, by the staining reagents, by the chromogens, by the fixatives and by endogeneouse biotin present in tissues such as liver and spleen and kidney. Background Buster is used in place of normal sera and other blocking solution for removing background staining in both human and animal tissues.

    Background Buster is applicable IHC staining, to immunofluorescence staining and to in situ probe staining in both human and animal tissues. It is also applicable to flow cytometric assays.

    Background Buster is a must for animal tissue staining, it is especially essential when staining identical species tissue and antibodies such as mouse antibodies on mouse tissues (Mouse-on-Mouse) and Rabbit-on- Rabbit. A 30-minute incubation with Background Buster is recommended prior to the application of the primary antibody for staining of identical species primary antibodies and tissues. The use of Background Buster is highly recommended for staining of indirect species (non-identical species tissue/ antibody) such as Rat –On-Mouse, Mouse-on- Rat, Mouse-on-Rabbit, etc. A 20-minute incubation with Innovex Background Buster is recommended prior to the application of the primary antibody for staining of Indirect (non-identical species primary antibodies and tissues).

    In immunoperoxidase-IHC staining, another type of background and non-specific staining is caused by red blood cell staining; this is due to endogenous peroxidase enzyme present in red blood cells. This type of background requires a pre-treatment step with 3% freshly made hydrogen peroxide (H2O2) in water, this blocking step should precede the blocking step with Innovex Background Buster.

    NB306 Background Buster 125 ml qty 419 EUR
    NB306-50 Background Buster 50 ml qty 325 EUR

    For more information Download PDF file

    Immunolocalization of electrogenic sodium-bicarbonate cotransporters pNBCI and kNBCI in the rat eye

    IMMNLINOLOCALIZATION  OF SODIUM.BICARBONATE  COTRANSPORTERS

    More: Background Buster

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