Today we officially introduce XerumFree™ XF205.

After more then a year of development and testing internally and externally we kicked off production.

The 5 times concentrated XF205 will bring you nothing but advantages. Both technically and commercially.  

XerumFree™ XF205 will give you:

Results
Being concentrated, adding XF205 has a minimum impact on a basal medium. If you completely or partially (for autocrine growth) change the medium there will be only  little disruption of the concentration. 

Ease of use
In most cases 2% directly added to your ready basal medium will be sufficient, lower or higher amounts for special applications are no problem. Stored cold means ready to use; no more hassle to thaw and test. Just use it straight from the bottle.

Consistency 
Only fully defined media give predictable results: supplements with animal derived components and e.g. hydrolisates are not consistent. There is no need for batch testing; every bottle will be exactly the same. 

Cost efficiency 
Not only the savings on storage, testing and logistics count but using XerumFree™ means that experiments with expensive reagents or ingredients will not fail due to the medium. Repeatable results in your own or other facility are saving valuable time and budget. The extreme low protein content makes downstream processing cheaper.

Freedom to operate
No growth factors, no hormones, no animal components. Adding XF205 to a basal medium gives you a platform that is fully defined and the possibility to develop your application on top. Repeatable, safe and robust. 

FDA-ready 
Any application that will have to pass the regulatory bodies one day  (e.g. the FDA) benefits from the defined composition. The risks of presence of viruses or TSE is eliminated completely as no single component is of animal origin, and we certify that.

Quality
GMP produced is the best guarantee for quality. Produced in the Netherlands in a production facility authorized by the ministry of Health we can assure quality from bottle to bottle and from lot to lot.

Universal
XerumFree™ can be used with most cell lines, primary cells and stem cells. Making it the best choice to streamline cell culture routines and at the same time reducing the need for special media.

Strategic choice
The coming years are predicted to make FBS scarce, changing to XerumFree™ is a strategic choice for availability in the future.

Weather it is to replace FBS or any other undefined supplement:
   
XerumFree™ XF205 simply makes fully defined easy.

Adapting Cells To a Serum-Free Environment

Fully defined, animal-component free and GMP produced cell culture supplement.

Performing cell culture without serum can be challenging. However, the rewards do largely recompense the efforts, and re-discove- ring the basics of cell culture develops quickly into a passion. The intention of this paper is to guide the user to a smooth transition to serum-free conditions and to avoid all inadequate or inappropriate efforts.

Ideally, the transition to serum-free conditions should be carried out over several passages to gradually select cells that can grow under serum-free conditions. However, direct adaptation to serum-free environments may also work out successfully, provided that all crucial aspects are addressed properly.

Regardless of the method used, key concerns include the growth state of the cellular inoculum, cell seeding density, sub-cultivation techniques, and biophysical attributes of the cell culture system.

Our XerumFree™ serum replacement has been designed so as to be used in the same way as conventional cell culture sera, as a medium supplement. The concentration however is 5x higher, so typically you will use 2% to a basal medium. You will go through the same steps as usual.

Be the first to order this brand new product!

Download datasheet with instructions for use