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GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
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    GENTAUR France

     GENTAUR France SARL
    9, rue Lagrange, 75005 Paris 
    Tel 01 43 25 01 50 
    Fax 01 43 25 01 60
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    Gentaur Germany

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      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
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    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
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    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
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    GENTAUR Nederland

     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
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    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
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    GENTAUR Spain

     GENTAUR Spain
    Tel 0911876558
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    Genprice USA

    usa-flagGenprice Inc, Logistics
    547, Yurok Circle
    San Jose, CA 95123
    Phone/Fax: 

    (408) 780-0908 

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    GENPRICE Inc. invoicing/ accounting:
    6017 Snell Ave, Suite 357
    San Jose, CA. 96123

     

    Gentaur Serbia

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    GENTAUR Greece

    grGENTAUR Greece 

    Tel 00302111768494 
    Fax 0032 16 50 90 45

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    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
    Danmark +4569918806
    Österreich +43720880899
    Ceská republika Praha +420246019719
    Ireland Dublin +35316526556
    Norge Oslo +4721031366
    Finland Helsset +358942419041
    Sverige Stockholm +46852503438
    Magyarország Budapest +3619980547

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    neuro1

    neuro2
    a) NCSCs forming characteristic “lacunae” during
    proliferation on ASC’s NCSC media (10X)
    b) NCSCs at a cell density ready for passaging.(20X)

    Background: One of the defining characteristics of vertebrate development is the migration of neural crest cells from the neural tube. Neural crest cells give rise to a variety of tissues such as melanocytes, some heart vessels, cephalic neuroendocrine organs, connective tissue of the craniofacial skeleton and the neurons and glia of both the sensory and autonomic ganglia of the peripheral nervous systems. The study of neural stem cells and their progenitors, Neural Crest Stem Cells (NCSCs), have direct medical implications. Numerous pathologies, such as skeletal and nervous system disorders and peripheral neuropathies, arise from aberrant migration, specification or differentiation of neural crest cells. In order to assist in your neural crest cell studies, our NCSC media has been optimized to contain all the factors required to support NCSCs plated on fibronectin coated tissue culture surfaces.
    Product description: Add 2 mL of NCSC medium supplement to 498 mL of NCSC medium to produce 500 mL of NCSC media.
    Storage conditions for NCSC medium: -80°C; 1 year, 4°C; two weeks. Minimize exposure to direct light.
    Storage condition for NCSC supplement: -80°C; 1 year, 4°C; two weeks.
    Storage condition for NCSC media: -80°C; 1 year, 4°C; two weeks. Minimize exposure to direct light.
    Shipping: On dry ice.
    Recommended procedure:
    1. Thaw NCSC medium either overnight at 4°C or for several hours at RT.
    2. Thaw the NCSC medium supplement at RT.
    3. Using sterile technique, add the NCSC medium supplement to the NSCS medium. Mix thoroughly and store the newly made media at 4°C for up to two weeks. NOTE: NCSC medium can be filter sterilized if desired. Do NOT filter sterilize the NCSC medium supplement. Add this supplement directly to the NCSC medium.
    4. Prior to use, warm the media to RT in the tissue culture hood. Due to the complex composition of the NCSC media, we do not recommend warming the media in a 37 C water bath.

     Price: 80 EUR

    Order Button1

    Published in Promos

    Based on our proprietary TARGATT Technology for site-specific knock-in mouse generation, we developed a TARGATT system specifically for introducing gene(s) of interest into mammalian cell lines. The unique advantage of this system is that any gene of interest can be inserted efficiently into a defined, transcriptionally-active locus with high gene expression in a single copy fashion.

     TARGATT Features:

    • Site-specific insertion of any gene 
    • High integration efficiency
    • Single-copy integration
    • Stable expression
    • Fast: get your transgenic cell line in 3 months!

     

    targatt talen knockout genemodification

    targatt-antibodies-gentaur-knockin-knockout-mouse-1

    Targatt proprietary TARGATT Technology enables highly efficient site-specific gene integration in mammalian cells and animals. This technology uses PhiC31 integrase to insert any gene of interest into a specific docking site that was pre-engineered into an intergenic and transcriptionally active genomic locus. Our TARGATT Technology improves several aspects in the generation of transgenic cell lines and animals:

    1. High integration efficiency mediated by  PhiC31 integrase reduces time and cost
    2. Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high expression levels of the transgene
    3. Integration at intergenic region ensures that no internal genes are interrupted
    4. Single copy gene integration eliminates repeat-induced gene silencing and genomic instability
    5. Site-specific integration allows a precise comparison of the effects of the transgenes among different lines.

    TARGATT Technology can be utilized for a variety of applications including reporter gene expression, gene knockdown and disease models.

    targatt-antibodies-gentaur-knockin-knockout-mouse-3

    Published in Targatt services
    Monday, 11 March 2013 15:15

    Knock-in and Knock-out Rat

    Targatt provides a complete range of gene targeting services in rats to generate transgenic knock-in and knock-out rat models.

    Rats are more versatile research model animals than mice since they are physiologically more similar to humans. The recent isolation of rat embryonic stem (ES) cells opened the doors to the generation of transgenic rats and we are proud to be one of the first providers of an all-inclusive transgenic rat generation service. Our competence in gene-targeting vector design coupled with our expertise in culturing rat ES cells allows us to offer a high-quality customized service for the generation of transgenic knock-in and knock-out rats.

    Using our proprietary germline competent rat ES cell lines we can create your customized transgenic rat model, including

    • - Constitutive knockin/out
    • - Conditional knockin/out
    • - Inducible knockin/out
    • - Point mutations
    • - Random integration
    • - Insertion of reporter genes
    • - Humanized rat models
    • - Disease rat models
    • knock-in-knock-out-mouse-targatt-1

     

    We take great pride in offering you the highest level of service and we are committed to ensuring the success of your transgenic rat project. Our service includes an initial project assessment based on your model requirements. A dedicated PhD level project manager will discuss available gene targeting vector design strategies and will update you continuously throughout the project.

    We provide the most flexible customized service options whether you’d like us to do all the work or are on a tight budget and prefer doing some steps yourself.

     Full Transgenic Rat Generation Service Includes:

    • - Gene targeting vector design, construction and sequencing
    • - Rat ES cell targeting, screening and expansion of positive rat ES cell clones
    • - Karyotyping of targeted rat ES cells
    • - Chimera production via blastcyst injection
    • - Chimera breeding for germline transmission
    • - Genotyping of offspring
    • - Transfer of heterozygous targeted rats to the customer
    • - Optional: breeding to generate homozygous rats

     

    All our animals are housed in a facility that is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International, has an Assurance on file with the Office of Laboratory Animal Welfare (OLAW), and is a registered research facility with the U.S. Department of Agriculture.

    knock-in-knock-out-mouse-targatt-2

    We also offer Knock-in/Knock-out Mouse and Transgenic Mouse (Random Insertion) services. For more information please contact us.

     

    Published in Targatt services
    Monday, 11 March 2013 15:13

    Random Transgenic Mouse

    We provide a service to generate transgenic mice by traditional pronuclear microinjection. Using this method, transgenes are inserted randomly into the genome.

    Expression of the transgene and the subsequent transgenic mouse phenotype may vary from line to line due to the nature of random gene insertion. We recommend to generate multiple independent trangenic mouse lines in order to interpret the experimental results.

    Available mouse strains: FVB, C57BL/6

    We also offer Knock-in and Knock-out Mice and Knock-in and Knock-out Rat services. For more information please contact us.

    Generation of transgenic mice by random insertion

    Step 1: Cloning of cDNA, promoter of interest, and other elements

    Step 2: Construction of the transgene vector

    Step 3: Pronuclear microinjection of vector DNA into mouse embryos

    Step 4: Transfer of injected embryo into recipient mothers

    Step 5: Genotyping of offspring to identify potential transgenic founders

    Step 6: Breeding to F1 or F2 progenies (optional)

    Published in Targatt services
    Monday, 11 March 2013 15:06

    Knock-in / Knock-out Mouse (7-months)

    We provide a high quality service for the generation of gene-targeted knock-in and knock-out mouse models. Our team has decades of experience in vector design, ES cell targeting and mouse handling. We will discuss your project needs with you and custom design your knockin/knockout mouse model. Our service includes consultation on available gene targeting vector design strategies and project assessment based on your model requirements.

    Service Milestones for the Generation of Knock-in/Knock-out Mouse Models

    • - Gene targeting vector construction and sequencing
    • - ES cell targeting, screening and expansion of positive ES cell clones
    • - Karyotyping of targeted ES cells
    • - Cre/FLP recombination (optional)
    • - Chimera production by blastocyst injection
    • - Breeding of chimeras for germline transmission
    • - Genotyping of offspring
    • - Transfer of heterozygous targeted mice to the customer
    • - Optional: breeding to generate homozygous mice

     

    Features

    Cost-effective

    • - Targatt has years of experience and an exceptionally high success rate in generating conventional gene-targeted mice with our proprietary mESC lines.

     

    High-Quality

    • - Our scientists have extensive experience in gene targeting and genetic mouse models. Our services always include a rigorous quality control with multiple check-points to ensure your projects are completed with highest accuracy. All mice are generated in and shipped from our California facility (NIH guidance certified).

    Please contact us with any questions regarding services not listed. We are happy to discuss your project with you and design customized strategies. We also offer Transgenic Mouse (Random Insertion) and Transgenic Rat Services (knockin, knockout).

    Testimony of Dr. Zhenheng Guo, PhD, Assistant Professor of Internal Medicine, Division of Endocrinology and Molecular Medicine, University of Kentucky

    Project: Germline-transmitted, conditional knock-out mice by tetraploid complementation

    "Overall, I am very satisfied with the quality of your service. After we received the mice, we did extensive studies to characterize them. All of the PCR reactions confirm the gene modifications are in the correct sites. We have also crossed mice with Cre mice to demonstrate that the Cre deleted the exon in heterozygous pups as expected. All data obtained showed that the mice were correctly targeted. In addition, I am very happy with the frequent communication with your scientists during the process. I highly recommend your services."

    Project types

    Express gene "X"

    Replace gene "X" with gene "Y"

    Delete gene "X"

    Random Integration

    Knockout/Knockin

    Knockout

    Knockin

     

    Conditional/inducible Knockout

    Conditional/inducible Knockin

       

    TARGATT

    (Site-Specific Knockin)

       

     

    Knock-in Mouse Models

    The introduction of a gene into a specified location of the mouse genome can be used for various applications. Mice can be homozygous or heterozygous for the inserted gene.

    • - Reporter genes (e.g. GFP, lacZ) are used for expression analysis of a gene of interest. The reporter gene is inserted into the gene of interested in-frame (non-disruptive), allowing for visualization of temporal and spatial gene expression pattern.
    • - Humanized disease models can be generated by inserting a human mutant gene or gene fragment into the corresponding mouse gene. The diseased human allel is thus transcribed in the appropriate genomic context and can be analyzed on behavioral, pathological, cellular and/or molecular level.
    • - DNA recombinases (CRE, FLP) can be inserted into a gene of interest. The celltype-specific expression of the recombinase then allows for gene inactivation in the desired tissue after crossing this knock-in mouse with a conditional knockout mouse.

     

    Knock-out Mouse Models

    The knockout technology is most commonly used for gene inactivation, either in a constitutive or conditional fashion.

    • - Constitutive knock-out mouse models are widely used to study gene function. The gene of interest is permanently inactivated in all cells of the animal. However, this non-conditional knock-out may cause lethality and is therefore not always recommended.
    • - Conditional knock-out models are inducible - the targeted gene is excised after crossing the mouse with a Cre-transgenic mouse line. Gene inactivation can be celltype-specific and/or chemically induced.

     

    Published in Targatt services

    esc
    Above: hESC in ESC-Sure
    conditioned medium
    Bottom: hESC on MEF TM

    Background: Traditionally human embryonic stem cell (hESC) culture requires a mouse embryonic fibroblast (MEF) cells’ support, which makes the protocol complicated. Our Serum- and Feeder-free medium that is for human ESC culture contains all the growth factors needed in hESC culture, except the bFGF; eliminate the routine preparation of feeder. It is a ready-to-use product for a serum/feeder-free system. Our medium makes your cell culture more efficient and easier to control.
    Serum- and Feeder-free medium (1x) for proliferation of pluripotent human embryonic stem cell (hESC) culture in a serum/feeder-free system. Each and every batch has been tested for hES/iPS cells pluripotency. All you need to add is 20 ng/ml bFGF.
    Product description: 100 mL of hESC-Sure
    Source: Chemical plus growth factors
    Storage: -80°C
    Shipping: Shipped on dry ice.
    Recommended procedure:
    Human ESC culture procedure using hESC-Sure Serum- and Feeder-free medium:
    1. Coat culture dish with Matrigel the day before.
    2. Wash Matrigel (BD Biosciences Cat# 354277) coat dishes with DMEM/F12 medium(Targatt Cat# ASM-5002).
    3. Once human ES cell were confluent or cultured for more than 5 days, disaggregated cells
    with 1mg/ml Dispase(Dilute 5mg/ml stock solution with DMEM/F12 media), incubate at 37
    C for 3 minutes.
    4. Wash container with 1x PBS, and to scrape colonies off the bottom of the container with cell
    scraper.
    5. Collect the cells in the 50ml or 15ml falcon tube, pellet by centrifugation at 700rpm for 5 min
    at room temperature.
    6. Resuspend hESC pellets in hESC-Sure Serum- and Feeder-free culture medium supplemented with 20 ng/ml bFGF.
    TM
    7. Split cells at 1:3 to 1:5 ratio every 4 to 5 days using 1mg/ml Dispase (diluted with
    DMEM/F12).
    8. 1x freezing medium contains 90% knockout serum and 10% DMSO.

     Price: 104 EUR

    Order Button1

    Published in Promos
    Monday, 11 March 2013 15:01

    TARGATT Knock-down Mouse

    Gene knock-down using short hairpin RNA (shRNA) to inhibit expression of the corresponding gene has been widely used in gene function studies, drug discovery, and disease research.  Our TARGATT Technology allows us to generate in vivo shRNA knock-down mouse models in 3 months.

    In addition to our full service we also offer individual service options to generate your TARGATT shRNA knock-down mouse.

    You can prepare the DNA using the TARGATT Kit and we do the pronuclear microinjection for you. Or we can prepare the DNA that you then inject after setting up your own TARGATT mouse colony.

    Please contact us to discuss your project plan for your fast & site-specific TARGATT Knock-in Mouse or knock-down mouse services.

    Features of TARGATT shRNA Knock-down Mouse Services:

    • - shRNA copy number is controlled.
    • - Site-specific insertion into high expression locus guarantees the expression of shRNA.
    • - shRNA expression can be regulated by a tissue-specific promoter.
    • - Founder mice can be generated within 3 months.
    • - Full service including shRNA design, construction, and validation is available.

     

    targatt-antibodies-gentaur-knockin-knockout-mouse-1

    Applied Stem Cell Inc.’s proprietary TARGATT Technology enables highly efficient site-specific gene integration in mammalian cells and animals. This technology uses PhiC31 integrase to insert any gene of interest into a specific docking site that was pre-engineered into an intergenic and transcriptionally active genomic locus. Our TARGATT Technology improves several aspects in the generation of transgenic cell lines and animals:

    1. High integration efficiency mediated by  PhiC31 integrase reduces time and cost

    2. Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high expression levels of the transgene

    3. Integration at intergenic region ensures that no internal genes are interrupted

    4. Single copy gene integration eliminates repeat-induced gene silencing and genomic instability

    5. Site-specific integration allows a precise comparison of the effects of the transgenes among different lines.

    TARGATT Technology can be utilized for a variety of applications including reporter gene expression, gene knockdown and disease models.

    targatt-antibodies-gentaur-knockin-knockout-mouse-3

    Published in Targatt services
    Monday, 11 March 2013 14:50

    TARGATT Knock-in Mice (3-months)

    Targatt can create site-specific knock-in mice for you within 3 months. Using our novel TARGATT system, a gene of interest can be inserted at a well-characterized, transcriptionally-active locus in the mouse genome with guaranteed transgene expression. Tissue-specific and/or ubiquitous expression options are available. Please contact us for a list of plasmid construct with reporter genes. 

    In addition to our full service we also offer individual service options to generate your TARGATT knock-in mouse.

    You can prepare the DNA using the TARGATT Kit and we do the pronuclear microinjection for you. Or we can prepare the DNA that you then inject after setting up your own TARGATT mouse colony.

    Please contact us to discuss your project plan for your fast & site-specific knock-in or TARGATT Knock-down Mouse services.

    * Prices are a general guideline for academic institutes and may vary.  Please inquire for quote.

    Advantages of TARGATT Technology:

    Site-specific DNA integration (knock-in) in transcriptionally-active locus

    • - Ensures robust gene expression
    • - Eliminates unpredictable phenotypes caused by random integration
    • - Allows for proper comparison of different transgenes

    Single-copy integration

    • - Eliminates repeat-induced gene silencing and genomic instability.

     

    Insertion of intact transgene

    • - Avoids truncated transgenes with unexpected phenotype

    High integration efficiency

    • Save time and costs!

     

    targatt-antibodies-gentaur-knockin-knockout-mouse-1

     

    Applied Stem Cell Inc.’s proprietary TARGATT Technology enables highly efficient site-specific gene integration in mammalian cells and animals. This technology uses PhiC31 integrase to insert any gene of interest into a specific docking site that was pre-engineered into an intergenic and transcriptionally active genomic locus. Our TARGATT Technology improves several aspects in the generation of transgenic cell lines and animals:

    1. High integration efficiency mediated by  PhiC31 integrase reduces time and cost

    2. Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high expression levels of the transgene

    3. Integration at intergenic region ensures that no internal genes are interrupted

    4. Single copy gene integration eliminates repeat-induced gene silencing and genomic instability

    5. Site-specific integration allows a precise comparison of the effects of the transgenes among different lines.

    TARGATT Technology can be utilized for a variety of applications including reporter gene expression, gene knockdown and disease models.

    targatt-antibodies-gentaur-knockin-knockout-mouse-3

    Published in Targatt services
    Wednesday, 06 March 2013 15:50

    Targatt transgenic Kit

    TARGATT Transgenic Kit is designed to create site-specific, knock-in transgenic mice in a more efficient and significantly faster way over traditional methods. Generating transgenic mice by conventional methods (e.g. pronuclear microinjection or lentiviral injection) has several limitations, first of which is random insertion of the transgene. Random insertion of a transgene results in a position effect where either the transgene is prone to silencing or endogenous gene expression is disrupted. Furthermore, this position effect is compounded when a transgene is inserted as multiple copies, resulting in instability at the insertion locus.

    Using our proprietary site-specific DNA integration system, TARGATT Transgenic Kit, combined with our genetically TARGATT mouse embryos (cat.# AST-0001, AST-0002, AST-0003, AST-0004). Targatt offers high quality H11(Hipp11) and ROSA26 transgenic mouse kits with our TARGATT technology. Using our TARGATT Technology, you can generate your own knock-in mouse in just three months.

    targatt-antibodies-gentaur-knockin-knockout-mouse-1

    targatt-antibodies-gentaur-knockin-knockout-mouse-2

    If you prefer to generate a knock-in mouse model on your own, TARGATT Embryo H11(Hipp11) or Rosa26 and Transgenic Kits (2 or 5 microinjection size) are available for purchase.

    Background:

    TARGATT technology enables highly efficient site-specific gene integration in mammalian cells and animals1, 2. This technology uses øC31 integrase to insert any gene of interest into a docking site, pre-engineered in an intergenic region and transcriptionally active genomic locus. Our TARGATT technology improves several aspects in the generation of transgenic cell lines and animals: (1) High integration efficiency mediated by øC31 integrase reduces time and cost; (2) Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high level expression of the transgene; (3) Integration at intergenic region ensures that no internal genes are interrupted; (4) Single copy gene integration eliminates repeat-induced gene silencing and genomic instability; (5) Site-specific integration allows a precise comparison of the effects of the transgenes among different lines. TARGATT technology can be utilized for a variety of applications including reporter gene expression, gene knockdown, disease cell and animal models.

    targatt-antibodies-gentaur-knockin-knockout-mouse-3

    Click here to see all products

    Published in Promos
    Wednesday, 06 March 2013 15:35

    TARGATT embryos

    TARGATT embryos are derived from one of our genetically engineered mouse models. These mouse embryos can be used as embryo donors for creating site-specific transgenic mice in a more efficient and faster way compared to traditional methods. Generating transgenic mice by conventional methods (e.g. pronuclear microinjection or lentiviral injection) has several limitations, first of which is the random insertion of the transgene. Random insertion of a transgene results in a position effect where either the transgene is prone to silencing or endogenous gene expression is disrupted. Furthermore, this position effect is compounded when transgenes are inserted in multiple copies, resulting in genomic instability at the insertion locus. Using our proprietary site-specific DNA integration technology, TARGATT embryos combined with our TARGATT Transgenic Kit (cat# AST-1001 or AST-1002), you can generate your desired transgenic mouse models with guaranteed gene expression faster.

    Gentaur offers high quality H11 and ROSA26 TARGATT frozen embryos. Using our TARGATT Technology, you can generate your own knock-in mouse from H11 or ROSA26 embryos in 3 months. Our H11(Hipp11) and ROSA26 frozen embryos contain 3 straws with a total 45-60 cryopreserved embryos (8-cell stage/morula). They are shipped in a dry shipper containing liquid nitrogen. We also offer fresh H11 and ROSA26 embryos with each vial containing 25-35 fresh embryos (E3.5) in KSOM medium. We offer overnight shipping at ambient temperature. Embryos will be transferred to recipient(s) immediately upon arrival. US customers only.

    targatt-antibodies-gentaur-knockin-knockout-mouse-1

    targatt-antibodies-gentaur-knockin-knockout-mouse-2

    If you prefer to generate a knock-in mouse model on your own, TARGATT Embryo (H11 or Rosa26) and Transgenic Kits (2 or 5 microinjection size) are available for purchase.

    Background:

    TARGATT technology enables highly efficient site-specific gene integration in mammalian cells and animals1, 2. This technology uses øC31 integrase to insert any gene of interest into a docking site, pre-engineered in an intergenic region and transcriptionally active genomic locus. Our TARGATT technology improves several aspects in the generation of transgenic cell lines and animals: (1) High integration efficiency mediated by  øC31 integrase reduces time and cost; (2) Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high level expression of the transgene; (3) Integration at intergenic region ensures that no internal genes are interrupted; (4) Single copy gene integration eliminates repeat-induced gene silencing and genomic instability; (5) Site-specific integration allows a precise comparison of the effects of the transgenes among different lines. TARGATT technology can be utilized for a variety of applications including reporter gene expression, gene knockdown, disease cell and animal models.

    targatt-antibodies-gentaur-knockin-knockout-mouse-3

    Click here to see all products

    Published in Promos
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