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Contact Us
 GENTAUR Europe BVBAVoortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45 Fax 0032 16 50 90 45 This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it. |
GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280
Fax 0035929830072
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GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50
Fax 01 43 25 01 60
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GmbH Marienbongard 20
52062 Aachen Deutschland
Tel (+49) 0241 56 00 99 68
Fax (+49) 0241 56 00 47 88 This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-family: Arial, Tahoma, Verdana, Helvetica; line-height: 15.59375px; ">
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GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531
Fax 020 8445 9411
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GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
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GENTAUR Nederland BV
Kuiper 1
5521 DG Eersel Nederland
Tel 0208-080893
Fax 0497-517897
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GENTAUR SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93
Fax 02 36 00 65 94
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GENTAUR Spain
Tel 0911876558
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Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
Phone/Fax:
(408) 780-0908
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GENPRICE Inc. invoicing/ accounting:
6017 Snell Ave, Suite 357
San Jose, CA. 96123
Serbia, 
Macedonia,
Montenegro, 
Croatia:
Tel 0035929830070
Fax 0035929830072
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GENTAUR Romania
Tel 0035929830070
Fax 0035929830072
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GENTAUR Greece
Tel 00302111768494
Fax 0032 16 50 90 45
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Other countries
Luxembourg +35220880274
Schweiz Züri +41435006251
Danmark +4569918806
Österreich +43720880899
Ceská republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Magyarország Budapest +3619980547
hESC-Sure Serum- and Feeder-Free Medium(SFFM)
Above: hESC in ESC-Sure
conditioned medium
Bottom: hESC on MEF TM
Background: Traditionally human embryonic stem cell (hESC) culture requires a mouse embryonic fibroblast (MEF) cells’ support, which makes the protocol complicated. Our Serum- and Feeder-free medium that is for human ESC culture contains all the growth factors needed in hESC culture, except the bFGF; eliminate the routine preparation of feeder. It is a ready-to-use product for a serum/feeder-free system. Our medium makes your cell culture more efficient and easier to control.
Serum- and Feeder-free medium (1x) for proliferation of pluripotent human embryonic stem cell (hESC) culture in a serum/feeder-free system. Each and every batch has been tested for hES/iPS cells pluripotency. All you need to add is 20 ng/ml bFGF.
Product description: 100 mL of hESC-Sure
Source: Chemical plus growth factors
Storage: -80°C
Shipping: Shipped on dry ice.
Recommended procedure:
Human ESC culture procedure using hESC-Sure Serum- and Feeder-free medium:
1. Coat culture dish with Matrigel the day before.
2. Wash Matrigel (BD Biosciences Cat# 354277) coat dishes with DMEM/F12 medium(Targatt Cat# ASM-5002).
3. Once human ES cell were confluent or cultured for more than 5 days, disaggregated cells
with 1mg/ml Dispase(Dilute 5mg/ml stock solution with DMEM/F12 media), incubate at 37
C for 3 minutes.
4. Wash container with 1x PBS, and to scrape colonies off the bottom of the container with cell
scraper.
5. Collect the cells in the 50ml or 15ml falcon tube, pellet by centrifugation at 700rpm for 5 min
at room temperature.
6. Resuspend hESC pellets in hESC-Sure Serum- and Feeder-free culture medium supplemented with 20 ng/ml bFGF.
TM
7. Split cells at 1:3 to 1:5 ratio every 4 to 5 days using 1mg/ml Dispase (diluted with
DMEM/F12).
8. 1x freezing medium contains 90% knockout serum and 10% DMSO.
Price: 104 EUR
