Suppliers
Contact Us
 GENTAUR Europe BVBAVoortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45 Fax 0032 16 50 90 45 This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it. |
GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280
Fax 0035929830072
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GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50
Fax 01 43 25 01 60
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GmbH Marienbongard 20
52062 Aachen Deutschland
Tel (+49) 0241 56 00 99 68
Fax (+49) 0241 56 00 47 88 This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-family: Arial, Tahoma, Verdana, Helvetica; line-height: 15.59375px; ">
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GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531
Fax 020 8445 9411
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GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
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GENTAUR Nederland BV
Kuiper 1
5521 DG Eersel Nederland
Tel 0208-080893
Fax 0497-517897
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GENTAUR SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93
Fax 02 36 00 65 94
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GENTAUR Spain
Tel 0911876558
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Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
Phone/Fax:
(408) 780-0908
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GENPRICE Inc. invoicing/ accounting:
6017 Snell Ave, Suite 357
San Jose, CA. 96123
Serbia, 
Macedonia,
Montenegro, 
Croatia:
Tel 0035929830070
Fax 0035929830072
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GENTAUR Romania
Tel 0035929830070
Fax 0035929830072
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GENTAUR Greece
Tel 00302111768494
Fax 0032 16 50 90 45
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Other countries
Luxembourg +35220880274
Schweiz Züri +41435006251
Danmark +4569918806
Österreich +43720880899
Ceská republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Magyarország Budapest +3619980547
AccuPower HotStart PCR PreMix
AccuPower HotStart PCR PreMix is a PCR master mix containing a thermostable DNA polymerase, thermostable pyrophosphatase, reaction buffer, dNTPs, tracking dye, and patented stabilizer in a ready to use hotstart PCR master mix.
Gentaur uses a unique enzyme-mediated HotStart PCR that provides robust and reliable results. Gentaur’s Top DNA Polymerase is inhibited at lower temperatures (< 70°C) by pyrophosphate. However, Top DNA Polymerase is rendered fully active at temperatures above 70°C via pyrophosphate hydrolysis with our thermostable pyrophosphatase. This prevents the formation of mis-primed products and primer-dimers during the reaction set up process resulting in improved PCR specificity. It is ideal for nucleic acid amplification reactions involving complex genomic or cDNA templates, very low copy targets, and multiplex reactions.
Features and Benefits
| Ease of use: | Just add template and primers and start your PCR, dNTPs, buffer and enzyme are provided |
| Stability: | Stable at room temperature for a month and for 2 years in a -20°C freezer |
| HotStart: | Unique enzyme mediated HotStart results in greater specificity and more robust reactions |
Specifications
5' to 3' exonuclease activity: No
3' to 5' exonuclease activity: No
Terminal transferase activity: Yes
Fragment Size: Up to 12 kb
Application
HotStart PCR up to 12 kb
PCR with complex genomic templates/low copy
Templates cDNA
Multiplex PCR reactions
Figure 1. Specificity comparison between AccuPower PCR PreMix and AccuPower HotStart PCR PreMix. 50 ng human genomic DNA was amplified into 2.5 - 3 kb fragments with the two products.
A: AccuPower PCR PreMix
B: AccuPower HotStart PCR PreMix
Lane M: 1 kb DNA Ladder (D-1040)
Lane 1: 2.5 kb product
Lane 2: 3.0 kb product
Figure 2. Specificity comparison of multiplex PCR with various HotStart PCR reagents. Each PCR mixture with 2 ng of human genomic DNA and 2 pairs of primers (p53 + p55 and p55 + p63) was incubated for 2 hours at 37°ree;C prior to performing multiplex PCR.
M: 100 bp DNA Ladder (D-1030)
A: AccuPower HotStart PCR PreMix
B: Control – AccuPower PCR PreMix
C: Company T's antibody mediated HotStart Taq DNA polymerase (0.5 U)
D: Company A’s antibody mediated HotStart Taq DNA polymerase (1.0 U)
