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    Displaying items by tag: Gentaur

    Researchers from the Hebrew University of Jerusalem and the Weizmann Institute of Science have developed a technique to cause apoptosis, or programmed cell death, that could lead to new approaches to treating cancer. 

    Apoptosis is an essential defense mechanism against the spread of abnormal cells such as cancer. It is a complex process that occurs through networks of proteins that interact with each other. Cancer cells usually avoid this process due to mutations in the genes that encode the relevant proteins. The result is that the cancer cells survive and take over while healthy cells die.

    The research, by graduate student Chen Hener-Katz at the Hebrew University, involved collaboration between Prof. Assaf Friedler of the Hebrew University's Institute of Chemistry and Prof. Atan Gross of the Weizmann Institute's Department of Biological Regulation. It was published in the Journal of Biological Chemistry under the title ''Molecular Basis of the Interaction between Proapoptotic Truncated BID (tBID) Protein and Mitochondrial Carrier Homologue 2 (MTCH2) Protein.''

    The study examined the interaction between two important proteins involved in cell death: mitochondrial carrier homologue 2 (MTCH2), which was discovered in the lab of Prof. Gross, and truncated BID (tBID), which are both involved in the apoptotic process. The researchers found the regions in the two proteins that are responsible for binding to each other, a critical step in initiating apoptosis. Following their discovery, the researchers developed short synthetic protein fragments, or peptides, that mimicked the areas on the proteins that bind to each other, and by doing so inhibited this binding. In lab experiments conducted on cell cultures, this resulted in the death of cancer cells of human origin.

    ''These protein segments could be the basis of future anti-cancer therapies in cases where the mechanism of natural cell death is not working properly,'' said Prof. Friedler. ''We have just begun to uncover the hidden potential in the interaction between these proteins. This is an important potential target for the development of anticancer drugs that will stimulate apoptosis by interfering with its regulation. ''

    Prof. Friedler is the head of the school of chemistry at the Hebrew University. His major research interests are using peptides to study protein-protein interactions in health and disease, and developing peptides as drug leads that modulate these interactions, specifically in relation to HIV and cancer. Prof. Friedler won the prestigious starting grant from the ERC (European Research Council) as well as the outstanding young scientist prize by the Israeli Chemical Society. His research was supported by a grant from the Israel Ministry of Health and by a starting grant from the European Research Council. 

    Published in News

    tobacco-plant-antibodiesSmoking tobacco might be bad for your health, but a genetically altered version of the plant might provide a relatively inexpensive cure for the deadly rabies virus. In a new research report appearing in The FASEB Journal, scientists produced a monoclonal antibody in transgenic tobacco plants that was shown to neutralize the rabies virus. This new antibody works by preventing the virus from attaching to nerve endings around the bite site and keeps the virus from traveling to the brain.

     

    "Rabies continues to kill many thousands of people throughout the developing world every year and can also affect international travelers," said Leonard Both, M.Sc., a researcher involved in the work from the Hotung Molecular Immunology Unit at St. George's, University of London, in the United Kingdom. "An untreated rabies infection is nearly 100 percent fatal and is usually seen as a death sentence. Producing an inexpensive antibody in transgenic plants opens the prospect of adequate rabies prevention for low-income families in developing countries."

     

    To make this advance, Both and colleagues "humanized" the sequences for the antibody so people could tolerate it. Then, the antibody was produced using transgenic tobacco plants as an inexpensive production platform. The antibody was purified from the plant leaves and characterized with regards to its protein and sugar composition. The antibody was also shown to be active in neutralizing a broad panel of rabies viruses, and the exact antibody docking site on the viral envelope was identified using certain chimeric rabies viruses.

     

    "Although treatable by antibodies if caught in time, rabies is bad news," said Gerald Weissmann, M.D., Editor-in-Chief of The FASEB Journal. "This is especially true for people in the developing world where manufacturing costs lead to treatment shortages. Being able to grow safe, humanized antibodies in genetically modified tobacco should reduce costs to make treatments more accessible, and save more lives."

     

     

    Published in News

    Available: Naked mole rats for aging research laboratories
    Origin: South Africa

    Heterocephalus glaber
    Female 1 y old 2850 GBP
    Male 1 y old 2250 GBP


    Delivery 4 weeks (not available in all countries)

    HUSBANDRY STANDARDS FOR KEEPING NAKED_MOLE RATS IN CAPTIVITY
    David Wood (1), Raymond Mendez (2)
    (1) Mill Mountain Zoo, P.O. Box 13484, Roanoke, VA 24034-3484
    (2) Work As Play, P.O. Box 16485, Portal, AZ 85632

    INTRODUCTION
    rat1Naked mole-rats are small rodents which belong to the family Bathyergidae, the genus Heterocephalus, and finally the species glaber. There are 12 species in 5 genera of bathyergid mole-rats. Scientist consider porcupines, chinchillas and guinea pigs to be their closest living relatives. The name Heterocephalus glaber means “different-headed hairless” (Rüppell, 1842). The naked mole-rat is a mammal because of body structure and lifecycle but is unlike other mammals. They have very little body hair and are unable to maintain a constant body temperature when the environmental temperature changes more then a few degrees. Furthermore, the naked mole-rat is unique among mammals because of its Eusocial lifestyle (Jarvis, 1981). Naked mole-rats are genetically very closely related to each other (Honeycutt et al. 1991a). Naked mole-rats are endemic to hot, dry regions of East Africa ranging from Kenya to Somalia and Ethiopia (Honeycutt et al. 1991b). Adults have a head and body length of 3.2-3.6 in (8 – 9 cm), tail length is 1.4-1.6 in (3.5 – 4 cm), and body weight of 1.4-2.8 oz (40 – 80 g) (Jarvis et. al. 1991: Lacey and Sherman 1991). Naked mole-rats can be long lived, in captivity some animals have survived over 26 years (and counting) (Sherman, Jarvis, 2002).

    rat2Historically the naked mole-rat had not been kept in North American Zoo collections until the late 1980’s. The species became desirable in zoological collections after the 1981 publication of their Eusocial behavior by Jarvis. The first few colonies that became available in the U.S. were surplus from Jarvis’s research at the University of Cape Town in South Africa. In 1991 additional naked mole-rats became available from a collecting expedition in Kenya conducted by Stan Braude and David Wood organized by the Philadelphia Zoological Garden. Although captive management techniques had been established for this species naked mole-rats presented a challenge in public exhibition because of the species sensitivity to sound and vibration (Brett, 1985). This had affected the successful rearing of offspring and normal behavior. Desensitizing the naked molerat for public exhibition with the use of radio proved successful (Wood, 1991). The exhibition of this unique mammal became very popular in Zoos not only for their educational value but also due to the Zoo visitors fascination with this species. Naked mole-rat exhibits quickly became successful as educational and entertaining exhibits as well as marketing promotions in North American Zoos.

    GENERAL REQUIREMENTS
    1. ABIOTIC ENVIRONMENTAL VARIABLES.

    1.1Temperature: Naked mole-rats are essentially poikilothermic evolving without the ability to thermo regulate their body temperature (Buffenstein and Yahav,1991a). Enclosure temperature range must be maintained between 82° - 87° F (26° - 31° C.) allowing the animals to regulate through heat exchange (Brett, 1986, 1991b). Higher or lower temperatures for extended periods will effect the health of the colony and may result in death.
    1.2 Humidity: Naked mole-rats should be maintained in a humidity level of 30-50%. Burrow humidity levels of 80% and higher have been recorded in the wild (McNab, 1966; Withers and Jarvis, 1980). If the humidity level drops too low for extended periods the mole-rats will develop dry skin. The appearance is that of a fine whitish covering over the skin.
    1.3 Illumination: Naked mole-rats live a subterranean existence. They have reduced eyes and visual acuity is poor (Hefner and Hefner 1993). Components of the visual system are present corneal electroretinogram recordings revealed no physiological responsiveness (Hetling et al. 2000). Mole-rats show an initial response to bright lights but do not seem to be effected by natural, fluorescent or incandescent lighting long term. The long-term effects of UV light exposure is unknown, precautions should be taken to protect this species from direct sunlight.
    1.4 Space
    1.4.1 Naked mole-rat burrow systems in the wild can encompass nearly 2 miles (3.2 km) for a large colony. Colonies can number from 10 to 290 individuals, with an average of 75 – 80 animals per colony (Braude 1991a, 2000; Brett 1991a). The burrow system must provide everything the colony needs to survive - protection, food and warmth. In captivity naked mole-rats will exhibit normal behaviors and reproduce in much less space. However, there may be a direct relationship between the limits of an artificial burrow system and the number a colony will grow to in captivity. Mole-rats can be maintained simply in a large aquarium or metal water trough. By providing PVC tubing, boxes and woodchips, the mole-rats will establish a burrow system. Because of the difficulty in maintaining sanitation and proper temperature, they should only be maintained in these conditions on a temporary basis. Use of clear PVC tubes and chambers is the most common method (Jarvis, 1991). A series of 2” (5cm) clear PVC tubes connected by elbows and Ts terminating in round 8” (20.3cm) diameter or square 10” X 10” X 6” (25.4cm X 25.4cm X 15.2cm) clear chambers. These round or square chambers are fabricated with a fixed bottom and removable top for accessing the animals, cleaning and feeding. The tops of the chambers are drilled with a series of ¼” (6mm) holes to provide ventilation. Hydrostone and wire with a glass panel can be sculpted to create habitats that are realistic in appearance (Mendez, 1995).
    1.4.2 Temporary separation: Naked mole-rats live in a colony which shares a communal scent. Separation such as escapes may result in these individual(s) not accepted back into the colony, and they could be killed. Attempts can be made to reestablish individual(s) in the colony by placing them along with the entire colony in a bucket or other neutral container. The contents of their toilet chamber should also be placed in this container. The colony should remain in this container for at least one hour before being placed back into their habitat. Success with this method is varied and is dependent on the length of separation and the colonies demeanor. Naked mole-rats should never be separated intentionally for more the a few minutes.
    1.4.3 Furnishings: Most members of a naked mole-rat colony spend their active time digging, foraging and maintaining their tunnel system. Items should be maintained in the burrow system to encourage these types of behaviors. Yam, potato and other large tubers can be used to block tunnels. Small cardboard boxes, cornhusk, clump grasses can be placed in a burrow or tunnel. Cardboard tubes from paper towels, toilet paper and wrapping paper inserted in the tunnel system work well. The activity of chewing and moving debris is fundamental to the mole-rat.
    1.4.4 Acoustic: Mole-rats can be sensitive to sound which will affect normal behavior and the successful rearing of offspring (Brett, 1985). Desensitizing mole-rats to sound can easily be accomplished with the use of a radio playing 24 hours a day (Wood, 1991). Volume should be set to approximately the same level as the sound they will be exposed to during exhibit maintenance and public exhibition.
    1.4.5 Substrates and bedding/nesting materials: Pinewood shavings, cornhusk, grasses and paper towel are materials normally provided for nesting material. Cedar shavings are not used due to its strong odor that may interfere with olfactory communication. Natural substrates such as sand or soil are not normally used because of the difficulty these cause in keeping the exhibit clean, dry and dust levels low which will effect viewing.
    1.4.6 Enclosure variation: Providing items within the habitat that simulate burrowing behaviors will greatly increase activity. Pine wood shavings, grasses, paper tubes, large tubers, small stones give the mole-rats items that can be moved but do not spoil the habitat. Blocking tube(s) with items such as yam or potato will also stimulate burrowing behavior. Mole-rats are relentless chewers, by providing them with controlled activities could possibly reduce exhibit repairs.
    1.4.7 Scent and cleaning: Habitats should be spot cleaned daily by removing old food and spoiled substrate. The entire habitat should be cleaned with mild dish soap every 2-4 weeks depending on the colony size. Mole-rats use a communal toilet chamber which is normally located at a dead end in the burrow system. This toilet chamber is important for the colony to maintain it’s olfactory identity and should not be excessively cleaned. The toilet chamber should be cleaned once a week by empting contents and rinsing with water, drying and adding new substrate. Toilets should be cleaned with mild dish soap about once a month.
    1.4.8 Air changes: Mole-rats having evolved in subterranean burrow systems and require little ventilation for good health. The naked mole-rat’s blood has a high oxygen affinity (Johansen et al. 1976) and the lungs are minimally developed (Maina et al. 1992). The number of air changes per hour should not effect the required temperature and humidity range in areas housing naked mole-rats. Vents or fans should not blow directly into mole-rat habitats.
    1.4.9 Containment: Naked mole-rats are relentless chewers and in time will chew through or damage most materials other then metal or glass. Mole-rat jaw muscles constitute 25% of the animals total muscle mass (T. Grand, vide Sherman et al. 1992).
    1.4.10 Transportation
    1.4.10.1 Type of transport container: Two types of containers are normally used when transporting molerats by air (Jarvis, 1991). A wooden box with a sheet metal lined interior, series of ¼” (6mm) ventilation holes drilled into the lid and a single row around all 4 sides. Holes drilled into the sides should be located near the top of the box, approximately ¾ the distance from the bottom. This will allow for proper ventilation but will prevent the mole-rats from chewing at the holes and possibly injuring themselves on the sheet metal. Lid is secured with screws. Metal box made from galvanized steel or aluminum with a hinged or fitted lid. Series of ¼” (6mm) ventilation holes drilled in a single row around all 4 sides approximately ¾ the distance from the bottom. The box is placed inside a standard Sky Kennel and secured by putting the two halves together. Foam padding is placed on the top and bottom of the metal box creating a tight fit inside the Sky Kennel.
    1.4.10.2 Container size: The size of the box is dependent on the number of animals being shipped. A box measuring 15” X 15” X 12” (38cm X 38cm X 30.5cm) can transport approximately 10 – 30 molerats. Smaller boxes should be used for fewer animals allowing them to huddle together for warmth. Larger boxes should be used for larger colonies to prevent overheating. When transporting naked mole-rats by car or hand carrying them, a plastic cooler works very well.
    1.4.10.3 Food and water: Mole-rats acquire all of their water needs from the food they eat (Urison and Buffenstein 1994). They should never be given water. Yam and some high in moisture fruits such as banana and apple should be provided during shipment.
    1.4.10.4 Bedding during transport: A layer of pinewood shavings 2-4” (5–10 cm) thick should be place in the container.
    1.4.10.5 Flooring: No special flooring is necessary to separate animals from their urine and feces, pinewood shaving will suffice.
    1.4.10.6 Temperature: Naked mole-rats are very sensitive to temperature and care must be taken to protect them from extremes. Effort should be made to ship molerats when the temperature range is 75-86°F (23-30º C) during transit. Communication with airport personnel regarding the naked mole-rats temperature sensitivity is important.
    1.4.10.7 Noise during transport: Effort should be made to minimizing nose and disturbance as much as possible during transport. Adult mole-rats can be very tolerant to these disturbances but very young animals are excessively carried and may not survive. It is advisable not to ship a colony of mole-rats with young less then 3 months old.
    1.4.10.8 Separation for transport: Mole-rat colonies should be kept together at all times but large colonies can be separated for some hours during transport. Colonies that have been separated for transport must be put together immediately upon arrival.
    1.4.10.9 Access during transport: Mole-rats can be safely transported by air without the need of accompanying staff. Arrangements should be made with airline personnel to take special precautions to maintain their temperature needs.
    1.4.10.10Duration of transport: Mole–rats shipped in closed containers by air transport should not exceed 24 hours. Mole-rats transported with access by animal care staff can take a number of days with a supply of food.
    1.4.10.11Release after transport: Mole-rats should be released into their exhibit or quarantine holding upon arrival. Colonies split for transportation must be reunited immediately.

    2. BIOTIC VARIABLES
    2.1 Food and water
    2.1.1 Water: Mole-rats acquire all of their water needs from the foods they eat even though some of these foods may be high in salts (Urison and Buffenstein 1994). Water should never be given to mole-rats.
    2.1.2 Food: In nature mole-rats feed on a variety of roots and tubers (Brett, 1991b). Natural food items are low-quality, high-fiber, digestive efficiencies are 88% (Buffenstein and Yahav 1991b). In captivity they do well on a base diet of sweet potato or yam. A combination of items such as banana, raisins, carrots, peas, corn, peach, apple, pear, frozen mixed vegetable should also be included.
    2.1.3 Feeding: Mole-rats should be presented food in one chamber, normally a dead end for the ease of removal and cleaning. They should have access to food 24 hours a day since individuals will feed at any time throughout the day.
    2.1.4 Enrichment feeding: Blocking a tunnel with foods such as yam, sweet potato, corn on the cob or carrot will provide activity as well as food.
    2.2 Social Considerations
    2.2.1 Group composition: Naked mole-rats have evolved with a highly structured social organization (Jarvis 1981). This organization consists of a caste system made up of a single breeding female, 1-3 breeding males, soldiers and workers. The breeding female or “queen” dominates the colony. Soldiers and workers can be of either sex but the colony sex ratio is male biased (Braude 1991a; Brett 1991a; Jarvis 1985; Sherman et al. 1992). Workers make up the largest group within the colony. All members of the colony help to care for the young and provide cecotrophes during weaning. Each individual naked mole-rat is in physical contact with the rest of the colony from birth to death. When the breeding female dies or is removed from the colony another female will take her place. During this transition, serious aggression may occur by competing females causing injuries and possible fatalities.
    2.2.2 Temporary isolation/separation: Naked mole-rats should never be Intentionally separated for more than a few minutes unless to found new colonies. Individuals can be separated for a few weeks and then reintroduced as founders for new colonies. Escaped animals can sometimes be reintroduced by following procedures outline in section 1.4.2.

    3. HEALTH and NUTRITION
    3.1 Diet
    3.1 Nutrient requirements: Naked mole-rats have a low metabolism feeding on a variety of roots and tubers in the wild (Brett 1991b). Sweet potato or yam is the staple in a mole-rat captive diet. Combinations of items such as corn, apple, raisins, pear, peach, carrot, banana, peas and frozen mixed vegetables should be added daily. Individuals will feed at anytime throughout the day. Fresh food must be accessible at all times.
    3.2 Health
    3.2.1 Preventative medicine: No vaccinations are currently recommended for rodents. Routine fecal checks should be performed twice yearly.
    3.2.2 Medical management: Mole-rat teeth continually grow but are normally worn down by tunnel excavation and by the filing of upper and lower against each other (Jarvis 1969; Lacey et al. 1991). With appropriate dietary components and this species tendency to chew on whatever is available no tooth clipping should be required. Teeth may grow too long and interfere with feeding if opposing teeth are damaged or lost. Trimming teeth with the use of toenail scissors is easily accomplished until missing teeth grow back. This procedure should be done as needed and the individual(s) returned to the colony immediately. Because of the rapid tooth growth in this species clipping may only be required for a few weeks.

    4. REPRODUCTION
    4.1 Breeding: The single breeding female (queen) mole-rat breeds with 1-3 breeding males throughout the year. Litters are normally born in 80 – 90 day intervals. Litter size can range from 1 - 24 pups but average litter sizes are 10 –19. Because of available space and the lack of predation in captivity naked mole-rats will stop raising litters. Periodic removal of non-breeding animals to establish new colonies can stimulate the successful rearing of litters. It is advised to allow the colony to self regulate its size.
    4.2 Neonate care: Naked mole-rat colonies become very sensitive to disturbance when newborns are present (Brett 1986). Pups are moved and carried excessively when the colony is disturbed. This causes injuries and does not allow the pups to nurse. Routine care and noise should be kept to a minimum. If possible, all activity except for feeding should cease until the pups are 15 – 20 days old. Colony members will stay with the pups keeping them together in one chamber. The “queen” will enter this chamber allowing the pups to nurse. Pups will solicit cecotrophes from colony members when they are 2 – 3 weeks old becoming independent at 4 – 6 weeks.
    4.3 Sexing: Naked mole-rats can be sexed by holding the animal upside down by the tail, look between the two orifice for the presents or absences of a horizontal red line. The presents of a red line indicates a female. Sexing naked mole-rats can become easy with practice.

    5. BEHAVIOR MANAGEMENT

    5.1 Aggression: Mole-rats will periodically fight or spar with one another to establish or maintain their status within the colony. This type of fighting is usually minor with no serious injuries. Serious aggression can occur when, the breeding female has died and females compete to replace her, more than a pair has been separated to found a new colony, animal(s) which have escaped or been kept separate from the colony for too long and are returned. Newly established breeding female may attack her siblings once she has had surviving litters of her own. Molerats that are seriously attacked and injured may eventually be killed if left in the colony.

    5.2 Staff: Animal care staff should be trained in small mammal husbandry and provided with information regarding the unique care requirements and social structure for this species.

    Definitions
    Caste: Individuals within a colony belonging to a particular morphological type or age group, or that share a particular behavior pattern and perform the same specialized function.
    Cecotrophes: Soft, partially digested pellets of feces eaten to replenish protozoa and provide nourishment.
    Eusocial: Used of a social group in which members are fully integrated and cooperate in caring for young, with non-reproductive individuals assisting those involved in producing offspring, and in which different generations contributing to colony labor overlap.
    LITERATURE CITED BRAUDE, S. 1991
    The Behavior and Demographics of the Naked Mole-rat, Heterocephalus glaber. Ph.D. dissertation, University of Michigan, Ann Arbor, 211 pp. BRAUDE, S. 2000
    Dispersal and new colony formation in wild naked mole-rats: evidence against inbreeding as the system of mating. Behavioral Ecology 11:7 – 12. BRETT, R. A. 1985
    Captive breeding and management of naked mole-rats Heterocephalus glaber. Proc. Symp. Assoc. Br. Wild Animal Keepers 10:16 – 11 BRETT, R. A. 1986
    The ecology and behavior of the naked mole-rat Heterocephalus glaber (Rüppell) (Rodentia: Bathyergidae). Ph.D. dissertation, University of London, United Kingdom, 391pp BRETT, R. A. 1991
    The population structure of naked mole-rat colonies. Pp. 97 – 136 in The biology of the naked mole-rat (P. W. Sherman, J. U. M. Jarvis, and R. D. Alexander, eds.). Princeton University Press, New Jersey BUFFENSTEIN, R., and S. YAHAV. 1991a.
    Is the naked mole-rat, Heterocephalus glaber, an endothermic yet poikilothermic mammal? Journal of Thermal Biology 16:227-232. BUFFENSTEIN, R., and S. YAHAV. 1991b.
    The effect of diet on microfaunal population and function in the caecum of a sub- terranean mole-rat, Heterocephalus glaber. British Journal of Nutrition 65:249-258

     

     

    Published in News

    Gene silencing by RNA interference (RNAi) technology using small interfering RNA (siRNA) is a powerful tool for studying functional genomics, drug target identification and validation, pathway elucidation, and therapeutic development. The advantage is in the algorithm we use for siRNA design. Our Turbo si-Designer software identifies highly effective siRNA target sites with remarkably high siRNA knockdown rates. Critical parameters including base composition, thermodynamic instability and base preference are all considered in the design algorithm. siRNAs spanning SNP sites are removed and finally, non-specific siRNAs are eliminated following homology searching by BLAST and Smith-Waterman algorithms. The result is an siRNA with extraordinary siRNA knockdown efficiency and minimal off-target effects. The performance of Turbo si-Designer has been extensively evaluated by testing the siRNA knockdown efficiency of thousands of siRNAs targeting STE Kinases, TK Kinases, genes involved in the NF-kappaB and Caspase Pathways using Real-time PCR analysis. The results of the evaluation indicated the design algorithm was highly effective in selecting effective siRNAs; 80% of the tested siRNAs showed > 70% knockdown and 38% elicited knockdown of > 90%.

     

    Cat. No. Guaranteed Yield Purification
    S-1017-5 1 nmole BioRP
    S-1017-6 5 nmole
    S-1017-1 10 nmole
    S-1017-2 20 nmole
    S-1017-3 50 nmole
    S-1017-4 100 nmole
    S-1018-5 1 nmole HPLC
    S-1018-6 5 nmole
    S-1018-1 10 nmole
    S-1018-2 20 nmole
    S-1018-3 50 nmole
    S-1018-4 100 nmole

     

    - siRNA provided lyophilized and annealed.
    - Annealing buffer is provided when single-stranded siRNA requested.

     

     

    Published in Promos
    Friday, 21 June 2013 15:30

    AccuTarget Control siRNAs

     

    AccuTarget™ Positive Control siRNAs

    Cat. No. Product Description Purification Guaranteed Yield
    SP-1001 AccuTarget™ GAPDH Positive Control siRNA BioRP 5 nmole
    SP-1002 AccuTarget™ GAPDH Positive Control siRNA BioRP 10 nmole
    SP-1003 AccuTarget™ GAPDH Positive Control siRNA BioRP 20 nmole
    SP-1011 AccuTarget™ GAPDH Positive Control siRNA HPLC 5 nmole
    SP-1012 AccuTarget™ GAPDH Positive Control siRNA HPLC 10 nmole
    SP-1013 AccuTarget™ GAPDH Positive Control siRNA HPLC 20 nmole
    SP-2001 AccuTarget™ GFP Positive Control siRNA BioRP 5 nmole
    SP-2002 AccuTarget™ GFP Positive Control siRNA BioRP 10 nmole
    SP-2003 AccuTarget™ GFP Positive Control siRNA BioRP 20 nmole
    SP-2011 AccuTarget™ GFP Positive Control siRNA HPLC 5 nmole
    SP-2012 AccuTarget™ GFP Positive Control siRNA HPLC 10 nmole
    SP-2013 AccuTarget™ GFP Positive Control siRNA HPLC 20 nmole
    SP-3001 AccuTarget™ Luciferase Positive Control siRNA BioRP 5 nmole
    SP-3002 AccuTarget™ Luciferase Positive Control siRNA BioRP 10 nmole
    SP-3003 AccuTarget™ Luciferase Positive Control siRNA BioRP 20 nmole
    SP-3011 AccuTarget™ Luciferase Positive Control siRNA HPLC 5 nmole
    SP-3012 AccuTarget™ Luciferase Positive Control siRNA HPLC 10 nmole
    SP-3013 AccuTarget™ Luciferase Positive Control siRNA HPLC 20 nmole



    AccuTarget™ Negative Control siRNAs

    Cat. No. Product Description Purification Guaranteed Yield
    SN-1001 AccuTarget™ Negative Contol siRNA BioRP 5 nmole
    SN-1002 AccuTarget™ Negative Contol siRNA BioRP 10 nmole
    SN-1003 AccuTarget™ Negative Contol siRNA BioRP 20 nmole
    SN-1011 AccuTarget™ Negative Contol siRNA HPLC 5 nmole
    SN-1012 AccuTarget™ Negative Contol siRNA HPLC 10 nmole
    SN-1013 AccuTarget™ Negative Contol siRNA HPLC 20 nmole
    SN-1021 AccuTarget™ Fluorescein-labeled Negative Control siRNA HPLC 5 nmole
    SN-1022 AccuTarget™ Fluorescein-labeled Negative Control siRNA HPLC 10 nmole
    SN-1023 AccuTarget™ Fluorescein-labeled Negative Control siRNA HPLC 20 nmole



    AccuTarget™ Control siRNA Sets

    Cat. No. Product Description Purification Guaranteed Yield
    SS-1001 AccuTarget™ GAPDH Control siRNA Set BioRP 5 nmole positive control
    + 2 nmole negative control
    SS-1002 AccuTarget™ GFP Control siRNA Set BioRP 5 nmole positive control
    + 2 nmole negative control
    SS-1003 AccuTarget™ Luciferase Control siRNA Set BioRP 5 nmole positive control
    + 2 nmole negative control
    SS-1011 AccuTarget™ GAPDH Control siRNA Set HPLC 5 nmole positive control
    + 2 nmole negative control
    SS-1012 AccuTarget™ GFP Control siRNA Set HPLC 5 nmole positive control
    + 2 nmole negative control
    SS-1013 AccuTarget™ Luciferase Control siRNA Set HPLC 5 nmole positive control
    + 2 nmole negative control
    Published in Promos

    brain scanner 3d gentaurAn international research team has produced the first-ever ultra-high resolution 3D digital reconstruction of a complete human brain. At the astonishingly low resolution of 20-microns, the new scans are providing an unprecedented glimpse into the inner workings of the mind.

    And remarkably, it could also be seen as a precursor to brain preservation and mind uploading. But more on that in just a bit. First, the breakthrough.

    It’s called BigBrain, and it’s a part of the $1.6 billion European Human Brain Project that's seeking to simulate the human brain on a supercomputer. Over the course of the next ten years, HBP researchers will work to understand and map the network of over a hundred billion neuronal connections that elicit emotions, volitional thought, and even consciousness itself. And to do so, the researchers will be using a progressively scaled-up multilayered simulation running on a supercomputer.

    But to get there, the researchers are going to have to peer deep inside the human brain. Hence the BigBrain project. 

    The Map is Not the Territory

    There is a risk, however, of overstating the importance of this breakthrough.

    As it has often been said, anatomy is not explanation. Just because we have a remarkably fine map of the human brain doesn’t mean that we’ll be able to understand it. No doubt, it will certainly help. But neuroscientists will still need to confer with cognitive scientists and other specialists if we ever hope to gain a full understanding of the human mind.

    The researchers are also unduly optimistic when it comes to their timelines. They plan to simulate the entire human brain — from the molecular level to the interaction of entire brain regions — on a supercomputer in ten years. I think that’s highly unlikely. But they are on the right track by developing these sorts of techniques.

    Published in News
    Friday, 21 June 2013 11:46

    Genes Get in Your Eye

    Genes-Eye-gentaurUsing mouse eyes as a setting for directed evolution, scientists have created a new version of the gene therapy vector adeno-associated virus (AAV) that can deliver genes deep into the retina, according to a paper published online today (June 12) in Science Translational Medicine. Such a vector could improve therapeutic gene delivery to target cells and lead to safer and less invasive gene therapy treatments.

    “This is a beautifully planned, executed, and powerfully presented paper,” said Jean Bennett, a professor of ophthalmology at the University of Pennsylvania in Philadelphia, who was not involved in the study. “It shows the results of a very clever system to evolve AAV to target cells in the retina efficiently from an intravitreal injection.”

    Intravitreal injection, whereby a needle is pushed into the eye’s vitreous, or gel-like core, is a common drug delivery procedure performed under local anesthetic in a doctor’s office, explained Bennett. But using this routine injection technique in trials of gene therapy for retinal degeneration has thus far proven impossible.

    The problem, explained David Schaffer, a professor of chemical and biomolecular engineering, bioengineering, and neuroscience at the University of California, Berkeley, who led the research, is that current AAV vectors are incapable of penetrating deep into the retina where the target cells for retinal diseases are located. “AAV is a respiratory virus and so it evolved to infect lung epithelial cells,” explained Schaffer. “It never evolved to penetrate deep into tissue.”

    Patients receiving gene therapy have therefore undergone a direct intraretinal injection, which requires hospitalization and general anesthetic, and can sometimes even damage the retina. If it were possible to inject AAV into the vitreous instead of the retina and still get gene delivery to the target cells, said Bennett, “one could envision the [doctor saying], ‘Ok, well just come into the office and get your gene therapy, tomorrow afternoon at two.’”

    With that aim, Schaffer and colleagues evolved AAV to be better at tissue penetration. They injected regular AAV into the vitreous of mouse eyes and one week later collected photoreceptor cells from deep within the retina. The tiny percentage of AAV vectors that made it into those cells were then amplified, repackaged into virus particles and injected into the vitreous again. They repeated the injection, recovery, and amplification a total of six times, finally isolating 48 AAV variants for sequencing. Two thirds of those isolates turned out to the same variant, and Schaffer and colleagues named it 7m8.

    The team then performed intravitreal injection of the 7m8 AAV vector to deliver missing genes into two mouse models of retinal degeneration—retinoschisis and Leber’s congenital amaurosis. In both models, the treated mice showed improved retinal function. Mice receiving their missing genes via intravitreal injection of the standard AAV vector, on the other hand, did not.

    Lastly, to determine whether the 7m8 vector would be likely to show similar deep penetration in the human retina, Schaffer injected the vector fused to a fluorescent protein into the vitreous of macaque eyes. Primate retinas are considerably thicker than those of mice, and the vector did not consistently reach the deep cell layers—showing a spotty penetration pattern rather than the wide and even pan-retinal penetration that had been seen in the mice. However, 7m8 did effectively target photoreceptor cells of the fovea—a thinner part of the primate retina that is essential for the sharp detailed vision humans use when reading and driving. “That’s a really important region to protect,” said Schaffer. “For the quality of life of patients who are going blind, if you can at least protect the fovea that would be a huge improvement.”

    Schaffer and colleagues don’t yet know what makes the 7m8 vector so much better at tissue penetration than its AAV ancestor, but they plan to find out and use that knowledge to further improve its penetration in the primate retina.

    They also plan to use similar directed evolution strategies to improve vector penetration into other body tissues “What this paper illustrates is the ability of purpose-directed vector evolution to achieve a specific anatomic transduction goal,” said Kathy High, a University of Pennsylvania professor of pediatrics who was not involved in the study. “And that’s an important development not just for ocular applications but for others like the liver or central nervous system.”

     

    Published in News

    bottles-gentaur-antibodiesBulgarian scientists have discovered a new disease. It is a neurological and is caused by mutation of genes, said Prof. Ivaylo Tarnev.

    New diagnosis called autosomal recessive congenital ataxia. It is accompanied by oftalmopareza and mental retardation.

    The disease is due to a mutation in a gene which encodes a metabotropic glutamate receptor 1 (mGluR1) on chromosome 6q24.

    The results of the Bulgarian medics were published in the prestigious scientific journal American Journal of Human Genetics.

    Published in News

    gentaur-dna 940Three new genes that lead to hereditary diseases found Bulgarian doctors and geneticists. For the first time achievement was announced at the ongoing forum in Albena on "Genes, brain, intelligence, behavior."

    Studies have been made over the past year and a half, despite the lack of state funding, the researchers were able to complete their work on the project. Describing the new neurological disease will lead to the exact diagnosis, and to the possibility of prophylaxis.

    "In families where there is a risk of being born with the same desease, already possible to prenatal diagnostic and diagnostic counseling in these families. All this can prevent them from occurring in new patients in these families, "explained Dr. Ivaylo Tarnev from University Hospital" Aleksandrovksa. "

    During the forum, the national consultant in medical genetics professor Ivo Kremenski will outline problems with the implementation of new genomic research into medical practice. Will be presented and advances in the treatment of brain tumors, genetics of behavior, the problems of people with autism.

    Published in News
    Friday, 14 June 2013 14:06

    CA 15-3 ELISA Kit

    ca15-3-elisa-kitINTENDED USE
    The CA15-3 for the quantitative determination of the Cancer Antigen CA15-3 concentration in human serum. This kit is intended for research use only.

    SUMMARY AND EXPLANATION
    Breast cancer is the most common life-threatening malignant lesion in women of many developed countries today, with approximately 180,000 new cases diagnosed every year. Roughly half of these newly diagnosed patients are node-negative, however 30% of these cases progress to metastatic disease.
    There are a number of tumor markers that can help clinicians to identify and diagnose which breast cancer patients will have aggressive disease and which will have an indolent course. These markers include estrogen and progesterone receptors, DNA ploidy and percent-S phase profile, epidermal growth factor receptor, HER-2/neu oncogene, p53 tumor suppressor gene, cathepsin D, proliferation markers and CA15-3. CA15-3 is most useful for monitoring patients post-operatively for recurrence, particularly metastatic
    diseases. 96% of patients with local and systemic recurrence have elevated CA15-3, which can be used to predict recurrence earlier than radiological and clinical criteria. A 25% increase in the serum CA15-3 is associated with progression of carcinoma. A 50% decrease in serum CA15-3 is associated with response to treatment. CA15-3 is more sensitive than CEA in early detection of breast cancer recurrence. In combination with CA125, CA15-3 has been shown to be useful in early detection of relapse of ovarian
    cancer. CA15-3 levels are also increased in colon, lung and hepatic tumors.

    PRINCIPLE OF THE TEST
    The CA15-3 ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes a monoclonal antibody directed against a distinct antigenic determinant on the intact CA15-3 molecule is used for solid phase immobilization (on the microtiter wells). A rabbit anti-CA15-3 antibody conjugated to horseradish peroxidase (HRPO) is in the antibody-enzyme conjugate solution. The test sample is allowed to react sequentially with the two antibodies, resulting in the CA15-3 molecules being sandwiched between the solid phase and enzyme-linked antibodies. After two separate 1-hour incubation steps at 37C, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of CA15-3 is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

     

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    Published in Top Products