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GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
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    GENTAUR France

     GENTAUR France SARL
    9, rue Lagrange, 75005 Paris 
    Tel 01 43 25 01 50 
    Fax 01 43 25 01 60
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    Gentaur Germany

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    52062 Aachen Deutschland
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    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
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    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
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     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
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    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
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    Tel 0911876558
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    Genprice USA

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    547, Yurok Circle
    San Jose, CA 95123
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    San Jose, CA. 96123

     

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    Tel 00302111768494 
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    Other countries
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    Displaying items by tag: Gentaur
    Friday, 01 March 2013 10:17

    AccuPower PCR Premix

    Gentaur’s AccuPower PCR PreMix is a convenient lyophilized PCR master mix containing Top DNA Polymerase, dNTPs, reaction buffer, tracking dye, and patented stabilizer. AccuPower PreMix PCR master mix includes our super-processive “three times faster than Taq” Top DNA Polymerase for the fastest nucleic acid amplification. While Top DNA Polymerase is ideal for applications where you would normally use Taq, if your needs include HotStart PCR, or High Fidelity PCR, please see our AccuPower HotStart PCR and AccuPower TLA product pages. AccuPower PreMix PCR master mix is available with or without tracking dye, depending on your application. If purchased with tracking dye, reactions can be loaded on agarose gels without adding loading buffer.

     

    Features and Benefits

    Ease of use: Just add template and primers and start your PCR, dNTPs, buffer and enzyme are provided
    Stability: Stable at room temperature for a month and for 2 years in a -20°C freezer
    Gel loading: Available with or without a tracking dye for ease of use

     

    Specifications

    5' to 3' exonuclease activity: No
    3' to 5' exonuclease activity: No
    Terminal transferase activity: Yes
    Fragment Size: Up to 10 kb


    Application

    Standard PCR

    1RT PreMix accu power elisa pcr gentaur

    Figure 1. Processivity comparison with Lambda DNA template and different PCR reagents.

     

    M: 100 bp Plus DNA Ladder (D-1035)
    A: Accupower PCR PreMix
    B: Taq mastermix from Company S
    C: Taq mastermix from Company T
    Lane M: 1 kb DNA Ladder (D-1040)
    Lane 1: 1 kb PCR product
    Lane 2: 2 kb PCR product
    Lane 3: 3 kb PCR product
    Lane 4: 4 kb PCR product

    2PreMix DualStar qPCR gentaur antibodies

    Figure 2. Comparison of sensitivity test for AccuPower PreMix PCR master mix and other companies' products using serial diluted human genomic DNA.

     

    Reaction condition: 95°C for 5 minutes, followed by 35 cycles of 20 seconds at 95°C, 20 seconds at 60°C, 30 seconds at 72°C.

    A: human Insulin receptor gene
    B: DNA cross-link repair 1A gene
    I: AccuPower PCR PreMix
    II: Taq DNA Polymerase from Company A
    III: Taq DNA Polymerase from Company Q
    IV: PCR PreMix from Company I
    M: 100 bp DNA ladder (D-1030)
    Lane 1: 10 ng
    Lane 2: 1 ng
    Lane 3: 100 ng
    Lane 4: 10 ng

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    Published in Promos
    Friday, 01 March 2013 10:17

    AccuPower Taq PCR PreMix

    The AccuPower Taq PCR PreMix is a convenient lyophilized PCR master mix containing Taq DNA polymerase, dNTPs, reaction buffer, tracking dye, and patented stabilizer and is aliquoted in 8-strip PCR tubes. 
    The premix retains its activity for over a month at room temperature and is stable for two years in -20°C freezer. 
    AccuPower Taq PCR PreMix is available with or without tracking dye, depending on your application. If purchased with tracking dye, reactions can be loaded on agarose gels without adding loading buffer.

     

    Features and Benefits

    Ease of use: Just add template and primers and start your reaction. All other reaction components including dNTPs, 
    buffer and enzyme are provided.
    Robust Stability: Stable at room temperature for a month, at 4°C for one year and for 2 years in a -20°C freezer.
    Versatility: The premix is ideal for a wide range of PCR applications.
    Gel loading: Available with or without a tracking dye. Samples with tracking dye can be loaded directly into gels after PCR
    Reproducibility: Gentaur's strict quality controlled production system ensures that your results will be reproducible experiment after experiment.

     

    Specifications

    Source: Thermus aquatics
    5' to 3' exonuclease activity: Yes
    3' to 5' exonuclease activity: No
    3' – A Overhang: Yes
    Fragment size: Up to 8 kb from human genomic DNA and 10 kb from Lambda DNA


    Applications

    - Routine PCR
    - Primer extension
    - TA cloning
    - Gene sequencing

    accupower-TaqPCR-gentaur-antibodies-cell-culture-pcr

    Figure 1. Comparison of PCR amplification efficiency between AccuPower Taq PCR PreMix from Gentaur and other suppliers' PCR master mix.

    The cycling conditions for AccuPower Taq PCR PreMix were 95°C for 5 min, 35 cycles of 95°C for 20 sec, 55°C for 20 sec, and 72°C for 30 sec. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
    Target Gene : Human myc.
    Lane M : 100 bp DNA Ladder (Gentaur, Cat. No. D-1030)
    Lane 1 : 10 ng of human total cDNA
    Lane 2 : 1 ng of human total cDNA
    Lane 3 : 100 pg of human total cDNA
    Lane 4 : 10 pg of human total cDNA

    accupower-TaqPCR-gentaur-antibodies-cell-culture-pcr-2

    Figure 2. Comparison of PCR amplification efficiency between AccuPower Taq PCR PreMix from Gentaur and other suppliers' PCR master mix.

    The cycling conditions for AccuPower Taq PCR PreMix were 95°C for 5 min, 35 cycles of 95°C for 20 sec, 55°C for 20 sec, and 72°C for 30 sec. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
    Target Gene: IRGC (Immunity-related GTPase family, cinema)
    Lane M : 100 bp DNA Ladder (Gentaur, Cat. No. D-1030)
    Lane 1 : 10 ng human genomic DNA
    Lane 2 : 1 ng human genomic DNA
    Lane 3 : 100 pg human genomic DNA
    Lane 4 : 10 pg human genomic DNA

    accupower-TaqPCR-gentaur-antibodies-cell-culture-pcr-3

    Figure 3. Comparison of PCR amplification of long targets between AccuPower Taq PCR PreMix from Gentaur and other suppliers' PCR master mix.

    The cycling conditions for AccuPower Taq PCR PreMix were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 65°C for 20 sec, and 68°C for 8 min. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
    Lane M : 1 kb DNA Ladder (Gentaur, Cat. No. D-1040)
    Lane 1 : 3 kb fragment (human tumor protein p53 gene) 
    Lane 2 : 4 kb fragment (human beta globin region)
    Lane 3 : 4.5 kb fragment (human DNA cross-link repair 1A gene)
    Lane 4 : 8 kb fragment (human hemoglobin epsilon 1 gene)

    Order Button1 

    Published in Promos

    antibodies-gentaur-genesUp to 10 per cent of the active genes of an organism that has survived 80 million years without sex are foreign, a new study from the University of Cambridge and Imperial College London reveals. The asexual organism, the bdelloid rotifer, has acquired a tenth of its active genes from bacteria and other simple organisms like fungi and algae.

     

    The findings were reported Nov. 15 in the journal PLoS Genetics.

     

    Bdelloid rotifers are best known for going 80 million years without sex, as they have evolved to reproduce successfully without males. Many asexual creatures go extinct without the benefit of traditional genetic evolution. However, bdelloids have flourished by developing ingenious ways of overcoming the limitations of being asexual.

     

    Bdelloids have also developed the fascinating ability to withstand almost complete desiccation when the freshwater pools they typically live in dry up. They can survive in the dry state for many years only to revive with no ill effect once water becomes available again.

     

    "We were thrilled when we discovered that nearly 10 per cent of bdelloids' active genes are foreign, adding to the weirdness of an already odd little creature," said Professor Alan Tunnacliffe, lead author of the study from the University of Cambridge. "We don't know how the gene transfer occurs, but it almost certainly involves ingesting DNA in organic debris, which their environments are full of. Bdelloids will eat anything smaller than their heads!"

    Because some of the foreign genes are activated when the bdelloids begin to dry out, the researchers believe that the genes play a role in bdelloids' ability to survive desiccation.

    Professor Tunnacliffe added: "Other researchers have shown that bdelloids contain powerful antioxidants, which help protect them from the toxic oxidising agents that are the by-products of desiccation. These antioxidants have not yet been identified, but we think that some of them result from foreign genes."

    For the study, the researchers extracted all of the messenger RNA (genetic code similar to DNA which provides a blueprint for the creation of proteins) from bdelloid rotifers and sequenced each message, creating a library of the animal's active coding information. Using a supercomputer, they then compared these messages with all other known sequences and found that in many cases similar sequences had been found in other organisms.

    Strangely, however, these other organisms were often not animals, but simple microbes. This means that bdelloids have genes that are not present in other animals, but have been acquired from micro-organisms and adapted for use in the rotifer.

    The research was funded by the Biotechnology and the Biological Sciences Research Council (BBSRC) and the European Research Council.

     

     

    Published in News

    antibodies-primary-secondaryAntibodies for :

    Arabidopsis thaliana, Chlamydomonas sp. , diatoms, Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Pisum sativum, Populus sp., Spinacia oleracea, Synechococcus sp., Zea mays. And many more!

    For: western blot, immunolocalizations, IP

    Speed up Your research with the most comprehensive plant and algal antibody collection from original manufacturer

    All antibodies are carefully validated by our own testing laboratory or in collaboration with scientists in the field. 

    Contact us and we will help you to find the right antibody for your application!

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    Published in Promos
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