Suppliers
Contact Us
 GENTAUR Europe BVBAVoortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45 Fax 0032 16 50 90 45 This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it. |
GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280
Fax 0035929830072
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GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50
Fax 01 43 25 01 60
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GmbH Marienbongard 20
52062 Aachen Deutschland
Tel (+49) 0241 56 00 99 68
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GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531
Fax 020 8445 9411
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GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
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GENTAUR Nederland BV
Kuiper 1
5521 DG Eersel Nederland
Tel 0208-080893
Fax 0497-517897
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GENTAUR SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93
Fax 02 36 00 65 94
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GENTAUR Spain
Tel 0911876558
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Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
Phone/Fax:
(408) 780-0908
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GENPRICE Inc. invoicing/ accounting:
6017 Snell Ave, Suite 357
San Jose, CA. 96123
Serbia, 
Macedonia,
Montenegro, 
Croatia:
Tel 0035929830070
Fax 0035929830072
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GENTAUR Romania
Tel 0035929830070
Fax 0035929830072
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GENTAUR Greece
Tel 00302111768494
Fax 0032 16 50 90 45
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Other countries
Luxembourg +35220880274
Schweiz Züri +41435006251
Danmark +4569918806
Österreich +43720880899
Ceská republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Magyarország Budapest +3619980547
AccuPower samples available!
| Cat. | Product | Price | Order |
| D-1020 SM | 25/100 bp mixed DNA ladder | 5 € |  |
| D-1040 SM | 1kb DNA Ladder | 5 € | |
| K-2631 SM | AccuPower ProFi Taq PCRpremix | 5 € | |
| K-2611 SM | AccuPower PyroHotstart Taq PCR premix | 5 € | |
| K-2301 SM | AccuPower Hotstart Pfu peR PreMix | 5 € | |
| K-2101 SM | AccuPower RocketScript RTPremix | 5 € | |
| K-2501 SM | AccuPower RocketScript RT-PCR premix | 5 € | |
| K-6704 SM | AccuPower Dual-HotStart RT-qPCR premix | 5 € | |
| K-6251 SM | AccuPower 2X GreenStar qPCRMaster Mix | 5 € | |
| K-6210 SM | AccuPower GreenStar qPCR PreMix | 5 € | |
| K-6100 SM | AccuPower DualStar qPCR PreMix | 5 € | |
More: AccuPower
AccuLadder 100 bp DNA Size Marker
More Sharp! High Resolution! More Convenience!
AccuLadder 100 bp DNA size marker was designed to determine the size of double stranded DNA fragments from 100 to 2,000 bp. AccuLadder is shaper and brighter than our standard 100bp ladder The AccuLadder 100 bp DNA size marker consists of 13 double stranded molecular weight markers ranging in sizes from 100 to 1,000 bp in 100 bp increments, and additional fragments of 1,200, 1,600, 2,000 bp. The 500, 1,000 and 2,000 bp bands are two times brighter for easy identification.
Note:
The DNA ladder can be applied directly onto an agarose gel.
There is no need to heat before loading. Repeated freezing and thawing should be avoided.
Features and Benefits
| Complete and ready to load: | Convenient |
| Easy-to-Identify reference bands: | Easy-to-read results |
| Competitive pricing: | Great value for your research dollar |
Specifications
Concentration: 54 ng/μl
Recommended loading: 4.0~5.0 μl / 5 mm lane width
Typical Number of lanes: 126~157 (5 mm lane width)
Size range (bp): 100 - 2,000
Number of bands: 13
Supplied in: 10 mM Tris-HCl (pH 8.0), 1 mM EDTA, 2.5% Ficoll, 0.005% Bromphenol Blue, 0.005% Xylene Cyanol
Storage: -20°C
2.0% TBE agarose gel stained with Ethidium Bromide.
Nuclease Activity Test
AccuPower TLA PCR PreMix
AccuPower TLA PCR PreMix is a high fidelity PCR master mix that contains an easy to resuspend lyophilized mix of TLADNA Polymerase, dNTPs, reaction buffer, tracking dye, and patented stabilizer conveniently packaged in individual tubes or plates. Simply add template, primers and water. The TLA DNA Polymerase in the premix is a cloned and modified derivative isolated from Thermococcus onnurineus NA1 and features a proofreading activity, an enhanced processivity, and robust amplification efficiency compared to Pfu DNA polymerase or Vent DNA polymerase. These features allow high fidelity DNA amplification as well as long range PCR up to 20 kb.
AccuPower TLA PreMix high fidelity PCR master mix is available with or without loading dye. When loading dye is present you can load it directly into your gel after PCR – no glycerol or glucose is required.
Features and Benefits
| Ease of use: | Just add template and primers and start your PCR, dNTPs, buffer and enzyme are provided |
| Stability: | Stable at room temperature for a month and for 2 years in a -20°C freezer |
| High fidelity: | For subcloning and other critical applications |
Specifications
5' to 3' exonuclease activity: No
3' to 5' exonuclease activity: Yes
3’ – A Overhang: No
Fragment Size: 20 kb
Application
Gene synthesis, PCR or primer extension requiring high fidelity, blunt end PCR cloning or mutagenesis, any amplification requiring high fidelity
Figure 1. Comparison of long PCR amplification between AccuPower TLA PCR PreMix and 2 high-fidelity PCR enzymes. Reaction mixtures were prepared and PCR cycling conditions were performed following the protocol recommendations.
Lane M1: 1 kb DNA Ladder (D-1040)
Lane M2: Lambda/Hind lll Marker (D-1050)
Lane 1, 8, 15: 2 kb PCR product
Lane 2, 9, 16: 5 kb PCR product
Lane 3, 10, 17: 8 kb PCR product
Lane 4, 11, 18: 10 kb PCR product
Lane 5, 12, 19: 12 kb PCR product
Lane 6, 13, 20: 15 kb PCR product
Lane 7, 14, 21: 20 kb PCR product
Figure 2. Comparison of amplification efficiency of AccuPower TLA PCR PreMix and other equivalent products PCR was carried out using AccuPower TLA PCR PreMix and 3 high-fidelity PCR enzymes from other companies. Parallel reactions were performed following the protocol recommendations, using 100 ng, 10 ng, 1 ng and 100 pg human genomic DNA. A 1 kb fragment of the human IR gene was amplified in 30 PCR cycles.
M: 100 bp DNA Ladder (D-1030)
Lane 1, 6, 11, 16: 100 ng of human genomic DNA
Lane 2, 7, 12, 17: 10 ng of human genomic DNA
Lane 3, 8, 13, 18: 1 ng of human genomic DNA
Lane 4, 9, 14, 19: 100 pg of human genomic DNA
Lane 5, 10, 15, 10: Negative control template
Figure 3. . Comparison of detection sensitivity between AccuPower TLA PCR PreMix, Taq DNA polymerase and 2 high-fidelity PCR enzymes in amplification of various sizes. Reaction mixtures were prepared and PCR cycling conditions were performed following the protocol recommendations.
M: 1 kb DNA Ladder (D-1040)
Lane 1, 6, 11, 16: 2 kb PCR product
Lane 2, 7, 12, 17: 4 kb PCR product
Lane 3, 8, 13, 18: 5 kb PCR product
Lane 4, 9, 14, 19: 7 kb PCR product
Lane 5, 10, 15, 10: 8 kb PCR product
Figure 4. . A 2 kb Lambda DNA fragment was amplified according to the protocol recommendations using various extension times ranging from 20 sec to 2 min.
M: 1 kb DNA Ladder (D-1040))
Lane 1, 6, 11, 16: 20 seconds of extension time
Lane 2, 7, 12, 17: 40 seconds of extension time
Lane 3, 8, 13, 18: 1 minute of extension time
Lane 4, 9, 14, 19: 1 minute 30 seconds of extension time
Lane 5, 10, 15, 10: 2 minutes of extension time
