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Contact Us
GENTAUR Europe BVBA Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45 Fax 0032 16 50 90 45 This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it. |
GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280
Fax 0035929830072
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GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50
Fax 01 43 25 01 60
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GmbH Marienbongard 20
52062 Aachen Deutschland
Tel (+49) 0241 56 00 99 68
Fax (+49) 0241 56 00 47 88 This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-family: Arial, Tahoma, Verdana, Helvetica; line-height: 15.59375px; ">
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GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531
Fax 020 8445 9411
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GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
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GENTAUR Nederland BV
Kuiper 1
5521 DG Eersel Nederland
Tel 0208-080893
Fax 0497-517897
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GENTAUR SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93
Fax 02 36 00 65 94
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GENTAUR Spain
Tel 0911876558
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Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
Phone/Fax:
(408) 780-0908
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GENPRICE Inc. invoicing/ accounting:
6017 Snell Ave, Suite 357
San Jose, CA. 96123
Serbia, Macedonia,
Montenegro, Croatia:
Tel 0035929830070
Fax 0035929830072
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GENTAUR Romania
Tel 0035929830070
Fax 0035929830072
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GENTAUR Greece
Tel 00302111768494
Fax 0032 16 50 90 45
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Other countries
Luxembourg +35220880274
Schweiz Züri +41435006251
Danmark +4569918806
Österreich +43720880899
Ceská republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Magyarország Budapest +3619980547
ProtoGel (30%) - 1 Litter
37.5:1 Acrylamide to Bisacrylamide Stabilized Solution
Optimized for SDS-PAGE of Proteins (Laemmli gels)
Consistently Crystal Clear Gels, Zero Fluorescence
Stabilized for Long Shelf Life
Catalog Number: EC-890
ProtoGel forms an electrophoresis matrix that is ideal for the separation of proteins and polypeptides. ProtoGel is a stabilized, ready-to-use 30% (w/v) acrylamide/methylene bisacrylamide solution (37.5:1 ratio), manufactured from the highest quality materials, from which virtually all impurities have been removed. ProtoGel has zero acrylic acid content, eliminating the fixed charges that cause band streaking. Additionally, oxidation products such as aldehydes have been removed by a selective adsorption process. Aldehydes can attack proteins, altering the structure of the sample, and thus altering Rf values. With ProtoGel, you can trust that your results will be consistent one electrophoretic run to the next.
Storage: ProtoGel is stable for 24 months when stored tightly capped in a dark area at room temperature (20°C).
ProtoGel (30%) Protocol
Measure and Mix Solutions:
The volume of ProtoGel required for gel casting solutions of any volume and acrylamide concentration may be calculated from the following formula:
Vp =(X) (Vt)/30
where:
Vp = Volume of 30% ProtoGel
X = % Monomer Desired in Gel
Vt = Total Volume of Gel Casting Solution
EXAMPLE: To make 100 ml of a 10% monomer gel, calculate the volume of Protogel to add as follows:
Vp = (10) (100)/30 = 33.3 ml
Initiate and Cast Gel:
For optimal results degas gel solution for 10 minutes under vacuum aspiration prior to innitiation with APS and TEMED. Add 1.0ml of 10% (w/v) ammonium persulfate for every 100ml of gel casting solution. Swirl gently to mix. Add 0.1 ml of TEMED for every 100ml of gel casting solution. Swirl gently to mix. Pour the solution into the gel casting cassette. The gel should begin to set in 10-20 minutes. To provide a sharp interface, overlay the gel with water saturated n-butanol during polymerization. Flush butanol away with water just before casting the stacking gel (below).
Cast Stacking Gel:
Use ProtoGel Stacking Buffer to make 10ml of a 4% stacking gel:
ProtoGel: 1.3ml
ProtoGel Stacking Buffer: 2.5ml
Deionized Water: 6.1ml
Add 0.05ml 10% Ammonium Persulfate and 0.01ml of TEMED. Gel will begin to set in 20 minutes.