With decades of experience our Molecular Biology Team has developed an exceptional expertise in anything DNA-related. We  can help you develop the best strategy to tackle your cloning projects and find solutions to all your technical problems. 

Some examples of our services:

• - Cloning of amplified DNA fragments
• - Gene targeting vectors for homologous recombination
• - Bacterial Artificial Chromosome (BAC) Recombineering
• - RNAi and inducible vectors
• - Site-specific mutagenesis
• - Tagging of genes
• - De novo gene synthesis

A typical cloning procedure includes:

• - Primer design for target region
• - PCR amplification of target region
• - Cloning of the PCR product into a cloning vector
• - Sub-cloning the gene into the vector of your choice
• - Picking, plating, culturing, and DNA preparation
• - Sequence confirmation
• - Plasmid purification (Mini, Midi, or Maxi size)

We offer standard and customized cell culture services including mycoplasma testing and FBS lot evaluation.

Please contact us to discuss your needs with our technical service specialists.

Targatt offers various services for the differentiation of ESC/iPSC lines into more specialized cells including multipotent stem cells and fully differentiated somatic cells.

Advantage of using multipotent stem cells

• Thaw ESC/iPSC-derived multipotent stem cells (e.g. Neural Stem Cells) when you need them
• Differentiate multipotent stem cells to specialized somatic cells (e.g. neurons and glial cells)
• Expand the multipotent stem cells for a ready supply for your planned experiments = save money and time!

Standard services:

• Neural Stem Cells
• Neural Crest Stem Cells
• Cardiomyocytes
• Skeletal Muscle

Bespoke services:

We also offer customized differentiation services. Please contact us with details about what kind of cells you are interested in.

Human ESC / iPSC Neuronal Differentiation Service

• Send us your pluripotent cell line (or use our iPSC generation service)
• We will differentiate your ESC/iPSC line into neural stem cells (NSCs) or neural crest stem cells (NCSC) by using our proprietary neural induction protocol.
• NSCs and NCSCs are proliferating cells that can be frozen and cultured over a prolonged period of time.
• NSCs can be further differentiated into multiple neural cell types.

Service to confirm pluripotency of human and mouse ESC and iPSC lines by Teratoma Formation Analysisafter injection of cells into an animal.

Features:

• - Turnaround time: 4 weeks (Mouse), 7 weeks (Human)
• - Number of cells required / injection site: Mouse ESCs/iPSCs = 0.5 x 10^6, Human ESCs/iPSCs = 1 x 10^6
• - Enhanced experimental protocol: Injection under both the kidney capsule and in testis results in higher efficiency of distinct teratoma formations with differentiated tissues derived from all three germ layers.

Service Includes:

• - Comprehensive report with histological analysis and high resolution publication-grade images of endoderm, mesoderm and ectoderm formation.
• - Tissue blocks and H&E stained tissue section slides

Notes:

• - Frozen sample culture 
• - Pathogen tests (please provide mycoplasma test result showing negative; otherwise additional testing fee will be required.) 
• - Kidney capsule tumor and testis tumor from each mouse will be embedded in one block. Separate blocks are available upon request.
• - Frozen tissue blocks are available.

Technical Details:

• Experimental setup: A total of 4 mice (3 experimental and 1 control) will be used.
• Injection site:Each recipient mouse will be injected at two locations: in the kidney capsule and in the testis.
• Service includes:Cell injection, teratoma harvesting, tissue preparation (embedding and sectioning), H&E staining and histology analysis of teratoma tissue sections.