Catalog number : DIUR-500
Quantity: 500 tests
Availability: Yes

DESCRIPTION:
Urea is primarily produced in the liver and secreted by the kidneys. Urea
is the major end product of protein catabolism in animals. It is the
primary vehicle for removal of toxic ammonia from the body. Urea
determination is very useful for the medical clinician to assess kidney
function of patients. In general, increased urea levels are associated with
nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal
diseases, e.g., congestive heart failure, liver diseases and diabetes.
Decreased levels indicate acute hepatic insufficiency or may result from
over-vigorous parenteral fluid therapy.
Simple, direct and automation-ready procedures for measuring urea
concentration or blood urea nitrogen BUN in biological samples are
becoming popular in Research and Drug Discovery. BioAssay Systems'
urea assay kit is designed to measure urea directly in biological samples
without any pretreatment. The improved Jung method utilizes a
chromogenic reagent that forms a colored complex specifically with urea.
The intensity of the color, measured at 520nm, is directly proportional to
the urea concentration in the sample. The optimized formulation
substantially reduces interference by substances in the raw samples.

KEY FEATURES:
Sensitive and accurate. Use 5 μL samples. Linear detection range 0.08
mg/dL (13 μM) to 100 mg/dL (17mM) urea in 96-well plate assay.
Simple and high-throughput. The procedure involves addition of a single
working reagent and incubation for 20 min. Can be readily automated as a
high-throughput assay for thousands of samples per day.
Improved reagent stability and versatility. The optimized formulation has
greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.
Low interference in biological samples. No pretreatments are needed.
Assays can be directly performed on raw biological samples i.e., in the
presence of lipid and protein.

APPLICATIONS:
Direct Assays: urea in serum, plasma, urine, milk, cell/tissue culture,
bronchoalveolar lavage (BAL) etc.
Drug Discovery/Pharmacology: effects of drugs on urea metabolism.
Environment: urea determination in waste water, soil etc.

KIT CONTENTS: (500 tests in 96-well plates)
Reagent A: 50 mL Reagent B: 50 mL
Urea standard: 1 mL 50 mg/dL
Storage conditions. The kit is shipped at room temperature. Store all
components at 2-8°C. For long-term storage, keep standard at –20°C.
Shelf life: 12 months after receipt.

Precautions: reagents are for research use only. Normal precautions for
laboratory reagents should be exercised while using the reagents.

 Price: 339 EUR

Catalog number : ISB01L
Quantity: 1L
Availability: Yes
Details:

InstantBlue™
InstantBlue is the fastest ready-to-use stain available and been optimised to give well-defined protein bands in a single step.
Specially formulated for ultra-fast, sensitive and safe detection of proteins, staining takes minutes without the need to wash, fix, microwave or destain.
Other benefits include high sensitivity (as little as 5 ng per band with BSA - See FAQs), low background staining and non-toxic formulation.
Ultra-fast staining - providing results in 15 minutes or less.
Single step procedure - no need to wash, fix, microwave or destain. Just take the gel out of the cassette and place in a container with InstantBlue.
High sensitivity - approximately 5-25ng of protein detected per band.
Super-Low background staining - Improves overall gel resolution and sensitivity and no possibility of over staining means that you can leave your gel in the stain indefinitely and still be able to read it.
Quantitative - Same gel-to-gel performance ideal for quantifying protein by densitometry
Non-toxic - ideal for regular use and disposal.
Mass Spec Compatible.

Introduction:
InstantBlue™ is a ready-to-use, proprietary Coomassie® stain that is specially
formulated for ultra-fast, sensitive and safe detection of your proteins. Protein
gels can be stained in minutes without the need to wash, fix or destain.
Only proteins are stained resulting in well defined blue bands on a highly
transparent background. The reduction of background interference results in a
better signal to noise ratio and may also have a positive impact on the overall
resolution and sensitivity.
The InstantBlue formulation is non-toxic and does not contain any methanol.
Proteins stained using the InstantBlue stain are also compatible with mass
spectrometry (MS) analysis.

Contents:
1L reagent, containing Coomassie dye, ethanol, phosphoric acid and solubilizing
agents in water. (Caution: Phosphoric acid is a corrosive liquid.)

Storage:
Upon receipt store at + 4°C. Discard any reagents that show discoloration or
evidence of microbial contamination. Be sure to keep the bottle capped when not
in use.

 Price: 144 EUR