Virus Core Antigen Detection

- Measures the p24 core protein of HIV-1
- Quantitation is performed on a standard microplate reader
- p24 Standard included

Overview: An anti-HIV p24 monoclonal coating antibody is adsorbed onto a microtiter plate. p24 antigen present in the sample or standard binds to the antibodies adsorbed on the plate; a FITC-conjugated mouse anti-p24 antibody is added and binds to p24 antigen captured by the first antibody.

Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-p24. Following unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and substrate solution reactive with HRP is added to the wells.

A colored product is formed in proportion to the amount of p24 antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant HIV-1 p24 protein and sample p24 concentration is then determined.

Storage: Store all kit reagents at 2° - 8°C. DO NOT FREEZE. When stored properly the kit is stable until the date indicated on the box label.

Download datasheet

Price: 486 Euro

Description: Recombinant Human IL-4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15000 Dalton. The rHuIL-4 is purified by proprietary chromatographic techniques.

Source : E.coli

Catalog number : 04-GMPhuIL4-50 µg

Molecular weight : 15 KDa

Identity : rh IL-4 as measured by Elisa and Western Blot

Specific activity : > 13.10 6 units/mg compared to NIBSC standard . CT-h4.S cell proliferation assay.

650 000 IU /vial

Purity : > 98% as determined by SDS-PAGE and HPLC

Endotoxin content : < 0.1 EU/ µg of IL-4 (LAL test)

Host DNA : < 10 pg/ng protein

Sterility : sterile according to EP Test 2.6.1.

Stabiliser : contains Trehalose ; no preservative.

Physical state : freeze-dried

Stability : 12 months at – 20°C to – 80°C

3 months after reconstitution with water for injection ( as defined in EP Monograph 0169) when stored at -80°C

Reconstitution : use 500 µL water for injection in class A environment in order to keep the GMP grade .

Packaging unit : 50 µg protein (Lowry test)

GMP

GENTAUR rh IL-4 is manufactured in full compliance with cGMP in facilities approved by the Belgian Ministry of Health for the production and storage of medicinal products.

Use: GENTAUR rh IL-4 is not an approved medicinal product and cannot be injected as such to patients.

FOR EX VIVO CELL CULTURE AND FOR IN VITRO RESEARCH USE ONLY

Download Gentaur's GMP Certificate of Analysis And MSDS

Price: 483 Euro

Exosomes are 60-120 nm membrane vesicles secreted by most cell types in vivo and in vitro. Exosomes are found in blood, urine, amniotic fluid, malignant ascite fluids, urine and in media from cells in culture. Exosomes contain distinct subsets of microRNAs depending upon the cell type from which they are secreted. Standard growth medium for most cells in culture require fetal bovine serum (FBS) as a growth supplement to DMEM. FBS is derived from bovine (cow) serum and contains a high abundance of cow exosome vesicles. These exosomes can interfere or cause significant background issues when studying the exosomes secreted from your cells of interest in standard culture conditions.

Exo-FBS supports equivalent growth of many types of cells in culture, is devoid of cow CD63 positive exosomes and does not have any measurable bovine microRNAs. Perform your studies of cellular secreted exosomes in culture without the worry of contaminating cow exosomes in your experiments. No ultracentrifugation required. Available as standard FBS supplement or heat inactivated FBS media supplement (treated at 65°C for 15 minutes before bovine exosome removal).

• Exosome-sized vesicles removed
• FBS that has been stripped of CD63 positive cow exosomes
• No detectable cow microRNAs
• Same cellular growth rates supported as standard FBS
• Use the same as standard FBS (add 10% in DMEM or RPMI)

ExoQuick benefits
• No time-consuming ultracentrifugation 
• Less expensive than costly Antibodies and beads 
• No complicated syringes required 
• Compatible with biofluid from any species 
• More effective than any other method 
• Isolate intact exosomes for functional studies 
• Use as little as 100 µl of serum 
• Start with 5 or 10 ml Media or Urine and use ExoQuick-TC

Click here for more information

The Bullet Blender Gold 50 combines dry ice cooling to keep samples near 4°C, noise insulation to keep homogenization quiet, and extra-powerful homogenization for tough samples. This homogenizer for tissue or cell cultures, can process up to 8 samples in parallel, each sample up to 3.5 g each in a 50 mL tube (Axygen® or Corning® brand 50 mL self standing tube with plug-seal cap).

Includes the following:

The Bullet Blender® Gold
Operator's manual
Basic starter kit which includes:
Bead Sample Pack BSP-50B contains 20mL centrifuge tubes of several different types of beads
Spoon for dispensing beads***

Cat.No: BB50-AU

Price: 4985 Euro

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More form Gentaur: Order Beads for Bullet Blender