Antigen: Tenascin C (TNC)

Synonyms: TN, HXB, MGC167029, Hxb, Ten, TN-C, AI528729, MGC144208, MGC144209, cytotactin, tenascin-C, C130033P17Rik, tenc, wu:fk04d02, TNC, tn, MGC140517

Quantity: 96 Tests/kit

Components:

1. Assay plate (12 x 8 coated Microwells). Quantity: 1(96 wells)
2. Standard (Freeze dried). Quantity: 2
3. Biotin-antibody (100 x concentrate) Quantity: 1 x 120 µl
3.HRP-avidin (100 x concentrate). Quantity: 1 x 120 µl
4. Biotin-antibody Diluent. Quantity: 1 x 10 ml
5. HRP-avidin Diluent. Quantity: 1 x 10 ml
6. Sample Diluent. Quantity: 1 x 20 ml
7. Wash Buffer (25 x concentrate). Quantity: 1 x 20 ml
8. TMB Substrate. Quantity: 1 x 10 ml
9. Stop Solution. Quantity: 1 x 10 ml
10. Adhesive Strip (For 96 wells). Quantity: 4
11. Instruction manual

Description Synonyms: CMD1Z, TNC, TNNC, cardiac troponin C|slow twitch skeletal/cardiac muscle troponin C|troponin C, slow|troponin C1, slow

Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Minimum Detection Limit: 0.195 ng/mL

Detection Range: 0.78 ng/mL - 50 ng/mL

Assay Precision: 
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.

Price: 1087 EUR

CA-19-9 (Gastrointestinal Cancer Antigen) ELISA Kit which is an enzyme-linked immunosorbent assay for the quantitative measurement of Gastrointensinal Cancer Antigen (CA-19-9) concentration in human serum. An elevated serum CA19-9 suggests the need for further clinical management. The CA-19-9 ELISA kit, like many ELISA KITS, is for diagnostic laboratory use only.

As in other Cancer ELISA Kits, the CA-19-9 ELISA kit is a solid phase two-site immunoassay. The CA-19-9 ELISA kit is intended to be used as a monitoring and screening test, and is useful for patients in clinical remission, as post-operative serum CA-19-9 values which fail to return to normal, strongly suggest the presence of residual tumor. Tumor recurrence is often accompanied by a rise of serum levels before progressive disease is clinically evident. The sensitivity of the CA-19-9 ELISA kit is 5 U/ml and the specificity is 97%.

Notable features of Cancer ELISA Kits:

▪ User-friendly directions and explanation of test procedures
▪ Simple and safe reagent preparation
▪ Clear instructions on specimen collection
▪ Comprehensive package of required materials
▪ Explicit quality control and storage guidelines
▪ Reliable and easy-to-read test results

Product inserts for most Cancer ELISA kits follow a similar method. See the CA-19-9 ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.

Gentaur also provides other ELISA Kits. 

For more information download PDF file 

Applications

 

• Routine PCR applications
• TA cloning

Description

BIOTAQ™ is widely used by molecular biologists that have come to depend upon the robust performance of this reagent.

BIOTAQ is a highly purified thermostable DNA polymerase offering very high yield over a wide range of PCR templates (Fig. 1), and is the ideal choice for most assays. BIOTAQ is a robust preparation and consistently delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.

BIOTAQ is supplied with 10x NH₄-based Reaction Buffer, which provides optimal conditions for most experiments. Additional MgCl₂ is provided to allow reaction conditions to be adjusted to suit the template.

The specificity and performance of BIOTAQ can be further improved with the use of 2x PolyMate Additive which is designed for GC- or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.

BIOTAQ™ DNA Polymerase is purified from Thermus aquaticus.
 

Unit Definition

One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.

Concentration

5u/µl

Storage Conditions

BIOTAQ can be stored for 12 months at -20°C.
 

Product Citations

1. Amaral, I.P. & Johnston, I.A. J. Exp. Biol. 214, 2125-2139 (2011).
2. Coutinho, C.P., et al. Infect. Immun. 79, 2950-2960 (2011).
3. Lora, J., et al. PNAS 108, 5461-5465 (2011).
4. Palazón, A., et al. Can. Res. 71, 801-811 (2011).
5. del Hoyo, A. & Pedrola-Monfort, Plant Systemat. Evol. 273(3-4), 151-67 (2008).

Best price on the market: 118 Euro for 500 Units

Download datasheet

Product name: HybridomaMax Growth Suppliment 
Catalog number: TX-HYB-02
Quantity: 11ml
Availability: Yes

The HybridoMax Hybridoma Growth Supplement replaces feeder layers or other conditioned media and growth factors used in hybridoma production. HybridoMax increases the yield of hybridomas surviving HAT selection, the cloning efficiency of B-cell hybridomas, and the number of antibody producing colonies.

The HybridoMax Hybridoma Growth Supplement is ready to use. Prepare the serum free Hybridoma growth medium by dissolving 1 bottle of HybridoMax (11 ml) into 1 Liter of D-MEM F-12 (Dulbecco's Modified Eagle's Medium F-12).

The prepared Serum-Free Hybridoma growth medium is a chemically defined culture medium supporting the serum-free growth of various hybridomas and 293 cells. The compounds of Hybridoma growth medium include water for injection, a modified DMEM/F12 base, HEPES buffer, known amounts of insulin, transferin, testosterone, sodium selenite, ethanolamine, a variety of saturated and unsaturated fatty acids, and stabilizing proteins. HybridoMax contains no IL-6 and no bovine serum albumine or other undefined protein mixtures.

Price: 137 €

HYB-05: 5 bottles for 645€
HYB-10: 10 bottles for 1150€

 Click here for Datasheet