We have purified and characterized polyclonal dog IgE. Serum IgE was precipitated by (NH4)2SO4 and then purified by two different procedures. Ion exchange on DEAE-Sephacel, followed by HPLC using Tonen hydroxylapatite and then Protein G-Sepharose, produced a highly purified IgE fraction (No. 1) free of IgG, IgA and IgM as measured by ELISA, but recovery of IgE as measured by passive cutaneous anaphylaxis was low. Gel filtration on Sephacryl S-300, Con A-Sepharose and Protein G-Sepharose recovered 18% of initial IgE, 0.02% IgG, 0.4% IgM and 0.3% IgA. This IgE fraction (No. 2) was used to induce antibody production in rabbits. Western blot analysis was then performed for dog IgE fractions No. 1 and 2. Using the rabbit anti-dog IgE, a prominent IgE band with an apparent molecular mass of 226 kD was identified in fractions No. 1 and 2 subjected to nonreducing SDS-PAGE. This band also reacted with anti-human IgE, but not with anti-dog IgG or anti-dog IgA. Under reducing conditions the approximate molecular mass for the IgE ε chain, estimated by Western blot using rabbit anti-dog IgE, was 73 kD, providing a molecular mass of 196 kD for dog IgE.

Price: 435 euro

More: Antibodies

The GENTAUR Bovine living cysticercosis ELISA is based on 2 mouse monoclonal antibodies that detect only protein of living Cystocerkosis infections. The ELISA can detect Cystocercosis from as low as 10 infections in one adult cattle on 500 ul of crude serum.

The results on Urine are not 100% accurate due to false positives.

Name: Bovine living cysticercosis ELISA

Product reference: 04-bov-cysto-ELISA

Price: 455 Euro / 96 wells

Use: Low detection of living infections, not quantitative but qualitative

Deze ELISA geeft een juiste diagnose in  100% van alle positieve gevallen, wanneer er meer dan 10 cysticercen aanwezig zijn in het karkas( keuringsverslag) Indien er tussen de 1 en 10 cysticercen aanwezig zijn daalt dit naar 65%. In theorie is 1 cysticerc voldoende om een mens te infecteren.

Name:  Rabbit polyclonal anti living bovine cystocercosis

Product reference: 04-bov-cysto-rab500

Price: 195 Euro / 500ul

Use: crude rabbit serum used for WB 1/500, ELISA capture antibody

Bovine cystocercosis is a parasitic disease that afflicts the muscles of cattle and is caused by larvae of the human tapewormTaenia Saginata. If people consume beef containing these parasites they can acquire intestinal tapeworm infections.

Detectietest ter confirmatie van de visuele postmortem diagnose van rundercysticercose

IWT project 080132

A Complete  Line for Several Applications

TRANSPLANTATION LINE

EPATITIS DELTA

MISCELLANY

ACCESSORY  KIT

MAIN FEATURES

• Kits contain all reagents necessary to carry out the test
• Internal controls to verify successful DNA extraction and amplification
• Quantitative tests: standard curve on 4 points
• High  Sensitivity and Specificity
• Small reaction volume: 5 ul
• Sample types: blood, serum and plasma, Liquor, Biopsies
• Storage temperature: +2/8°C (no  shipment in dry ice needed CE marked)
• Shelf life: 12 months
• Kits format: 25, 50, 100, 150 reactions: no waste

PROTOCOLS

Instruments protocols:

• ABI 7500 Sequence Detection System® (Software SDS version 1.3.1, Applied Biosystems™)
• Miniopticon® (Software CFX manager version 1.6, Biorad™)
• MX3000P® (Software MxPro version 4.01,  Stratagene™)

IN PROGRESS:
- CFX 96 (BioRad™)
- ROTOR-GENE Q (QIAGEN)
- SmartCycler (CEPHEID)

Click here for more information

More: PCR

A sampling of beads for use with micro-centrifuge tubes. Note that this pack is included with Bullet Blender® and Bullet Blender® Blue purchases. Glass beads: 0.1mm and 0.5mm. ZrO beads: 0.5mm, 1.0mm, and 2.0mm. Stainless steel: 0.2mm, 0.9-2.0mm blend and 3.2mm. 10ml of each.

For Bullet Blender 1.5mL tube models. Includes 10mL of: GB01, GB05, ZROB05, ZROB10, ZROB20, SSB02, SSB14B and SSB32. Includes a 0.1mL scoop and micro-spatula.

Price: 189 €

More: MicroCentrifuge tubes