GelRed™ is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed is far more sensitive than EB without requiring a destaining step (Figure 1). GelRed and EB have virtually the same spectra (Figure 3), so you can directly replace EB with GelRed without changing your existing imaging system.
GelRed™ can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. GelRed can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. GelRed is also compatible with downstream DNA manipulations such as restriction digest, sequencing, and cloning.
A series of safety tests have confirmed that GelRedTM is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal.
We offer GelRed™ 10,000X solution in DMSO and in water (cat#41003) for better safety. For your convenience, we also offer ready-to-use GelRedTM 3X in water (cat#41001) that can be directly used for post gel staining. For customers who look for large pack size, we offer a cost-saving bulk pack size of 10mL (cat#41003-1).

FEATURES:
Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
Passed environmental safety tests for direct disposal down the drain or in regular trash
Much more sensitive than EtBr and SYBR Safe
Available in water, stable at room temperature for long-term storage and microwavable
- Safer than EB
- Easy disposal
- Ultra-sensitive
- Extremely stable

Very simple procedures for either precast and post gel staining
GelRed replaces EtBr with no optical setting change; GelGreen replaces SYBR or GelStar with no optical setting change (see Figure 3)
Compatible with downstream DNA manipulations such as restriction digest, sequencing and cloning.
Simple to use
Perfectly compatible with a standard UV transilluminator
Perfectly compatible with downstream applications

Figure 1.GelRed™ is significantly more sensitive than ethidium bromide (EB) for detecting low-level DNA, especially in the lower molecular weight area. Shown left are two-fold serial dilutions of 1 Kb Plus DNA Ladder from Invitrogen electrophoresed on 1% agarose gels precasted with GelRed or EB in 1x TBE. The total amount of DNA loaded per lane was: 200 ng, 100 ng, 50 ng and 25 ng from left to right. Gels were imaged using 300-nm transillumination and photographed with an EB filter and Polaroid 667 black-and-white print films.

The Most Sensitive and Stable Precast Gel Stain

Figure 2. GelRed™ displays consistently superior sensitivity for post gel staining, regardless of the filter used (A vs. C) and storage and handling condition. SYBR Gold, however, showed comparable performance only when used fresh from the manufacturer and with a SYBR filter (B vs. D). Following a few freeze-thaw cycles, SYBR Gold 10,000X solution degraded significantly, resulting in poor staining (E). SYBR Gold 1X solution also degrades over time (see Figure 4). Two-fold serial dilutions of 1kb Plus DNA Ladder from Invitrogen were electrophoresed on 1% agarose gels in 1x TBE and post- stained with GelRedTMand SYBR Gold, respectively. Gels were imaged using 300-nm transillumination and photographed with the indicated filters and Polaroid black-and-white print films. The total amount of DNA per lane for each serial dilution was: 200 ng, 100 ng, 50 ng and 25 ng from left to right.

Figure 3. Excitation and emission spectra of GelRed and GelGreen in the presence of DNA in PBS buffer

Note: *GelRed and its uses are covered by pending US and international patents. **SYBR is trademark of Molecular Probes, Inc. and GelStar is trademark of FMC corporation.
Please also see our EvaGreen™(cat#31000), a breakthrough nucleic acid dye ideally suited for quantitative real-time PCR(qPCR). By incorporating a smart "release-on-demand" DNA-binding technology, EvaGreen™ has low PCR inhibition while exhibiting superior sensitivity. Similar to our GelRed™, EvaGreenTM has remarkable stability.
Reference:
For Electrophoretic Mobility Shift Assay: 1. Liu,Y.,et al. Biochemistry, DOI: 10.1021/bi902050p, 2010, 2. Konate, K., et al. Biochemistry, DOI: 10.1021/bi901791x, 2010.

Catalog number : 41003

Quantity: 0.5

Price: 180 Euro

The 25-Hydroxyvitamin D Total ELISA is powered by unique monoclonal antibodies that express 100 % reactivity against 25 OH Vitamin D3 and 83% reactivity against 25OH Vitamin D2.
All In One technology. No extraction step, displacement solution directly into the well. Results in less than 4 hours. Calibrated against ID-LC/MS-MS (reference method). Bar codes on all components.

Technical spec.:

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Protocol comparison with ELISA

Vibrio cholerae Z132, DNA (10 µg)

PRODUCT DESCRIPTION: Each aliquot contains 10 µg of DNA extracted from a pure culture of Vibrio cholerae. The identification of this organism was confirmed by 16S sequencing. The purity of the culture was monitored by Gram staining and by additional culturing. The DNA was extracted from the cells following the bacterial protocol from the Qiagen®Genomic DNA Handbook using Qiagen®Genomic DNA Buffers with a 500/G genomic tip.DNA concentration and A260/280 ratios are determined using a NanoDrop ND-1000®. The extracted DNA also tested positive on an inhouse real time PCR assay.

INTENDED USE: Purified Genomic DNA is designed for use as an amplification and/or detection control for nucleic acid testing of Vibrio cholerae. It can also be used to determine a limit of detection (LOD), in diagnostic assay development, cross-reactivity studies or genomic sequencing. When used as a control for nucleic acid tests, the same protocols as those used to amplify extracted clinical specimens should be employed.

PRECAUTIONS:
- Use Universal Precautions when handling Genomic DNA.
- The material may be re-frozen after thawing. Repetitive freezing and thawing is not recommended (aliquot material if necessary).
- To avoid cross-contamination, use separate pipette tips for all reagents.

RECOMMENDED STORAGE: This control is supplied in TE Buffer and should be frozen at -20°C or below.

DO NOT USE IN HUMANS: These products are intended for research, product development or manufacturing use only. These products are NOT intended for use in the manufacture or processing of injectable products subject to licensure under section 351 of the Public Health Service Act or for any other product intended for administration to humans.

Escherichia coli O111:NM, DNA (10 µg)

PRODUCT DESCRIPTION: Each aliquot contains 10 µg of DNA extracted from a pure culture of Escherichia coli. The identification of this organism was confirmed by 16S sequencing. The purity of the culture was monitored by Gram staining and by additional culturing. he DNA was extracted from the cells following the bacterial protocol from the Qiagen®Genomic DNA Handbook using Qiagen®Genomic DNA Buffers with a 500/G genomic tip.DNA concentration and A260/280 ratios are determined using a NanoDrop ND-1000®. The extracted DNA also tested positive on an inhouse real time PCR assay.

INTENDED USE: Purified Genomic DNA is designed for use as an amplification and/or detection control for nucleic acid testing of Escherichia coli. It can also be used to determine a limit of detection (LOD), in diagnostic assay development, cross-reactivity studies or enomic sequencing. When used as a control for nucleic acid tests, the same protocols as those used to amplify extracted clinical specimens should be employed.

PRECAUTIONS:
- Use Universal Precautions when handling Genomic DNA.
- The material may be re-frozen after thawing. Repetitive freezing and thawing is not recommended (aliquot material if necessary).
- To avoid cross-contamination, use separate pipette tips for all reagents.

RECOMMENDED STORAGE: This control is supplied in TE Buffer and should be frozen at -20°C or below.

Plesiomonas shigelloides Z130, DNA (10 µg)

PRODUCT DESCRIPTION: Each aliquot contains 10 µg of DNA extracted from a pure culture of Plesiomonas shigelloides. The identification of this organism was confirmed by 16S sequencing. The purity of the culture was monitored by Gram staining and by dditional culturing. The DNA was extracted from the cells following the bacterial protocol from the Qiagen® Genomic DNA Handbook using Qiagen® Genomic DNA Buffers with a 500/G genomic tip. DNA concentration and A260/280 ratios are determined using a NanoDrop ND-1000®. The extracted DNA also tested positive on an inhouse real time PCR assay.

INTENDED USE: Purified Genomic DNA is designed for use as an amplification and/or detection control for nucleic acid testing of Plesiomonas shigelloides. It can also be used to determine a limit of detection (LOD), in diagnostic assay development, crossreactivity tudies or genomic sequencing. When used as a control for nucleic acid tests, the same protocols as those used to amplify extracted clinical specimens should be employed.

PRECAUTIONS:
- Use Universal Precautions when handling Genomic DNA.
- The material may be re-frozen after thawing. Repetitive freezing and thawing is not recommended (aliquot material if necessary).
- To avoid cross-contamination, use separate pipette tips for all reagents.

RECOMMENDED STORAGE: This control is supplied in TE Buffer and should be frozen at -20°C or below.

Gene silencing by RNA interference (RNAi) technology using small interfering RNA (siRNA) is a powerful tool for studying functional genomics, drug target identification and validation, pathway elucidation, and therapeutic development. The advantage is in the algorithm we use for siRNA design. Our Turbo si-Designer software identifies highly effective siRNA target sites with remarkably high siRNA knockdown rates. Critical parameters including base composition, thermodynamic instability and base preference are all considered in the design algorithm. siRNAs spanning SNP sites are removed and finally, non-specific siRNAs are eliminated following homology searching by BLAST and Smith-Waterman algorithms. The result is an siRNA with extraordinary siRNA knockdown efficiency and minimal off-target effects. The performance of Turbo si-Designer has been extensively evaluated by testing the siRNA knockdown efficiency of thousands of siRNAs targeting STE Kinases, TK Kinases, genes involved in the NF-kappaB and Caspase Pathways using Real-time PCR analysis. The results of the evaluation indicated the design algorithm was highly effective in selecting effective siRNAs; 80% of the tested siRNAs showed > 70% knockdown and 38% elicited knockdown of > 90%.

- siRNA provided lyophilized and annealed.
- Annealing buffer is provided when single-stranded siRNA requested.