Suppliers
Contact Us
 GENTAUR Europe BVBAVoortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45 Fax 0032 16 50 90 45 This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it. |
GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280
Fax 0035929830072
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GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50
Fax 01 43 25 01 60
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GmbH Marienbongard 20
52062 Aachen Deutschland
Tel (+49) 0241 56 00 99 68
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GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531
Fax 020 8445 9411
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GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
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GENTAUR Nederland BV
Kuiper 1
5521 DG Eersel Nederland
Tel 0208-080893
Fax 0497-517897
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GENTAUR SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93
Fax 02 36 00 65 94
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GENTAUR Spain
Tel 0911876558
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Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
Phone/Fax:
(408) 780-0908
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GENPRICE Inc. invoicing/ accounting:
6017 Snell Ave, Suite 357
San Jose, CA. 96123
Serbia, 
Macedonia,
Montenegro, 
Croatia:
Tel 0035929830070
Fax 0035929830072
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GENTAUR Romania
Tel 0035929830070
Fax 0035929830072
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GENTAUR Greece
Tel 00302111768494
Fax 0032 16 50 90 45
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Other countries
Luxembourg +35220880274
Schweiz Züri +41435006251
Danmark +4569918806
Österreich +43720880899
Ceská republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Magyarország Budapest +3619980547
AccuPower Taq PCR PreMix
The AccuPower Taq PCR PreMix is a convenient lyophilized PCR master mix containing Taq DNA polymerase, dNTPs, reaction buffer, tracking dye, and patented stabilizer and is aliquoted in 8-strip PCR tubes.
The premix retains its activity for over a month at room temperature and is stable for two years in -20°C freezer.
AccuPower Taq PCR PreMix is available with or without tracking dye, depending on your application. If purchased with tracking dye, reactions can be loaded on agarose gels without adding loading buffer.
Features and Benefits
| Ease of use: | Just add template and primers and start your reaction. All other reaction components including dNTPs, buffer and enzyme are provided. |
| Robust Stability: | Stable at room temperature for a month, at 4°C for one year and for 2 years in a -20°C freezer. |
| Versatility: | The premix is ideal for a wide range of PCR applications. |
| Gel loading: | Available with or without a tracking dye. Samples with tracking dye can be loaded directly into gels after PCR |
| Reproducibility: | Gentaur's strict quality controlled production system ensures that your results will be reproducible experiment after experiment. |
Specifications
Source: Thermus aquatics
5' to 3' exonuclease activity: Yes
3' to 5' exonuclease activity: No
3' – A Overhang: Yes
Fragment size: Up to 8 kb from human genomic DNA and 10 kb from Lambda DNA
Applications
- Routine PCR
- Primer extension
- TA cloning
- Gene sequencing
Figure 1. Comparison of PCR amplification efficiency between AccuPower Taq PCR PreMix from Gentaur and other suppliers' PCR master mix.
The cycling conditions for AccuPower Taq PCR PreMix were 95°C for 5 min, 35 cycles of 95°C for 20 sec, 55°C for 20 sec, and 72°C for 30 sec. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
Target Gene : Human myc.
Lane M : 100 bp DNA Ladder (Gentaur, Cat. No. D-1030)
Lane 1 : 10 ng of human total cDNA
Lane 2 : 1 ng of human total cDNA
Lane 3 : 100 pg of human total cDNA
Lane 4 : 10 pg of human total cDNA
Figure 2. Comparison of PCR amplification efficiency between AccuPower Taq PCR PreMix from Gentaur and other suppliers' PCR master mix.
The cycling conditions for AccuPower Taq PCR PreMix were 95°C for 5 min, 35 cycles of 95°C for 20 sec, 55°C for 20 sec, and 72°C for 30 sec. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
Target Gene: IRGC (Immunity-related GTPase family, cinema)
Lane M : 100 bp DNA Ladder (Gentaur, Cat. No. D-1030)
Lane 1 : 10 ng human genomic DNA
Lane 2 : 1 ng human genomic DNA
Lane 3 : 100 pg human genomic DNA
Lane 4 : 10 pg human genomic DNA
Figure 3. Comparison of PCR amplification of long targets between AccuPower Taq PCR PreMix from Gentaur and other suppliers' PCR master mix.
The cycling conditions for AccuPower Taq PCR PreMix were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 65°C for 20 sec, and 68°C for 8 min. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
Lane M : 1 kb DNA Ladder (Gentaur, Cat. No. D-1040)
Lane 1 : 3 kb fragment (human tumor protein p53 gene)
Lane 2 : 4 kb fragment (human beta globin region)
Lane 3 : 4.5 kb fragment (human DNA cross-link repair 1A gene)
Lane 4 : 8 kb fragment (human hemoglobin epsilon 1 gene)
Primary and secondary antibodies for plant and algal cell biology
Antibodies for :
Arabidopsis thaliana, Chlamydomonas sp. , diatoms, Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Physcomitrella patens, Pisum sativum, Populus sp., Spinacia oleracea, Synechococcus sp., Zea mays. And many more!
For: western blot, immunolocalizations, IP
Speed up Your research with the most comprehensive plant and algal antibody collection from original manufacturer
All antibodies are carefully validated by our own testing laboratory or in collaboration with scientists in the field.
Contact us and we will help you to find the right antibody for your application!
Click for PDF documentp40 Antibody
The p40 antibody, catalog number A00112 is provided in concentrated form or in ready-to-use as a 2ml, 7ml, or 25ml. This antibody is very important as it is helpful in the indication of several different carcinomas and has a wide-spread use in the market. We have developed the p40 antibody as a replacement antibody for the p63 antibody. The marker is highly specific for squamous basal cells and is more specific than the p63 marker. One of the most important functions of this antibody is the ability to differentiate between adeno carcinoma and squamous cell carcinoma for lung cancer. As you are aware the difference between those carcinomas will determine what avenue of treatment will be applied. The p40 is specific for indication of the squamous cell carcinoma. We will soon be releasing our Napsin A antibody for the adeno carcinoma. These two antibodies work well in conjunction with one another and they will help the pathologist determine what method of treatment is necessary.
The p40 is also an excellent antibody in the use of prostate cancer. In the early stages of prostate cancer the basal cells inside the ducts will be stained by the p40. This will help indicate the prostatic intraepithelial neoplasia (PIN) that is considered to be a pre-malignancy as it is a carcinoma in situ of the prostatic glands. As the cancer progresses the basal cells will be seen throughout the tissue as the cells become invasive. This antibody can help determine the stage of carcinoma by indicating locations of the basal cells that are stained.
The p40 antibody is also being used with breast cancer as the antibody will stain myoepithelial cells along the ducts of the mammary glands. This antibody is excellent in determining if Atypical ductal hyperplasia (ADH) is present which can indicate a risk factor for breast cancer and serves as early detection of breast cancer. This marker will also indicate Ductal Carcinoma in Situ (DCIS) similar to the way it works on prostate cancer. This is the most common non-invasive type of breast cancer. The marker will indicate DCIS by highlighting that the cells are still within the ducts. As breast cancer progresses and becomes invasive those ducts will breakdown and the myoepithelial cells will be seen throughout the tissue and become Invasive Ductal Carcinoma (IDC).
p40 (p63 Delta); Polyclonal (Ready-To-Use)
Species: Rabbit
Clone: Polyclonal
Isotype: Rabbit IgG
Species Reactivity: Human.
Cellular Localization: Nuclear.
Specificity: The new marker p40 (p63 delta) is highly specific for squamous basal cells.
p40 (p63 Delta); Polyclonal (Concentrate)
Species: Rabbit
Clone: Polyclonal
Isotype: Rabbit IgG
Species Reactivity: Human.
Cellular Localization: Nuclear.
Specificity: The new marker p40 (p63 delta) is highly specific for squamous basal cells.
Heat Block Incubator
WSC-2610 MyMiniBLOCK
Do you need only heating function with block incubator? WSC-2610 MyMiniBLOCK is a microprocessor-controlled product using advanced thermoelectric technology. With this technique, more stable and accurate up to 100 C temperature control is achieved. In compact body, very various use such as sample preparation of electrophoresis, DNA/RNA denature and reaction, trans-information of E. coli, etc. can be applied.
WSC-2620 PowerBLOCK
WSC-2620 PowerBLOCK is able to reach set-temperature in mins.
It can reach to the target temperature in about 7min, you can easily use it with different temperature just after using with previously set temperature. Wide and stably accurate temperature setting is from –10 to 100o C. It can be used for very wide applications and used in different temperature settings continuously.
WSC-2630 PowerBLOCK Shaker
PowerChek™ Animal Species ID Real-time PCR Kits
Characteristics
· Detection kit for Animal Species
· Real-time PCR based on TaqMan™ probe-based technology
· Rapid & simple reaction
· No contamination : closed system
· No error : Internal positive control
· Easy to operation in less than 2.5 hrs
Kit Components
· Primer/probe mixture
· 2X Real-time PCR Master Mix
· Control
Protocol
After DNA Ext.
· Step 1 : Mix extracted DNA with the Kit’s reagents
· Step 2 : Real-time PCR run
· Step 3 : Analysis
Exp
Products:
|
R0406 |
PowerChekTM Horse species Real-time PCR Kit Fluorephore: FAM |
50 rxn |
383-Eur |
|
R0406E |
PowerChekTM Horse species Real-time PCR Kit with EPC Fluorephore: FAM, HEX(VIC) |
50 rxn |
441-Eur |
Kronos Dio
A luminometer for real-time reporter assay with 8 dish incubator and multicolor measurement system
A luminescence monitoring tool to observe gene expressions with bioluminescent markers under long-term culture. Can be used in studies of internal biological clock, circadian rhythms in organisms, gene silencing, drug stimulation, etc. Kronos Dio is equiped with cell culture system within its housing to provide real-time periodical luminescence measurement with multi-color luciferase assay, enabling users to analyse the effects of complex factors influencing gene expression in the cell or the tissue. Internal temperature control (range: 20 to 45 °C) and carbon dioxide circulation allows for convenient cell culture over a long period of time.
Price: 29 500 Euro / 36 000 USD / 27 600 GBP
Installation and training
For the price GENTAUR provides notebook, installation and training (demonstration of basic software and hardware functionality). 6 hours of installation and training is inclusive in the price at no extra cost for USA and Europe. The necessary materials and tools for connecting the device to the Carbone dioxide source at the expense of the customer.
See also: Cellgraph AB-3000B
Literature
Customer references:
Aaron Rowland, Ph.D.
Assistant Professor of Toxicology
Department of Chemistry & Biochemistry
New Mexico State University
1175 North Horseshoe Drive
P.O. Box 30001
Las Cruces, NM 88003-8001
We are wanting to explore the ability of human breast cell lines to exhibit circadian rhythm. We are also interested in influence of environmental toxicants on circadian rhythm as well as general gene regulation. I felt that the Kronos dio would be the proper equipment to perform this task as it has a CO2 hook up and can monitor 3 colors at once. Therefore, I could transfect the BASIC luciferase construct using one color and the gene of interest using a different luciferase and monitor reporter activity in real time.
Observation that Irritant of Environmental toxin on Human’s breast cell lines reaction and changes.
BULLET BLENDER
The BULLET BLENDER® enables you to homogenize, disrupt, or lyse up to 24 tissue or cell culture samples at a time. Load the samples into standard polypropylene tubes, then place them in the BULLET BLENDER. The "bullets" in the instrument vigorously strike all of the tubes and homogenize your sample in minutes. The Bullet Blender is available with Air Cooling™ to preserve your sample quality!
Applications:
Homogenize Tissue
Liver, Spleen, Kidney, Brain, Adipose, Bladder, Eye, Thyroid, Gallbladder, Muscle, Pancreas, Testes, Meconium, Thymus
Homogenize Tougher Tissue
Heart, Aorta, Tumor, Tail Snips, Colon, Lung, Blood Vessel, Trachea, Cartilage, Epithelium, Stomach, Intestine, Larynx, Mouse Femur, Pharynx, Umbilical Cord, Skin, Uterus
Lyse Cells
E. coli, Mamallian cell culture, Yeast
Homogenize Plant Material
Leaves, Beans, Nuts, Roots
Homogenize Small Organisms
Zebrafish, Drosophila
Whole Cell Isolation
Bacteria, Lymphocytes, Blood Cells
Organelle Isolation
Nucleus, Mitochondria
Click here for more information
More form Gentaur: Order Beads for Bullet Blender
Centrifugal Tubes
560 tubes of 15ml for only 25 Euro
440 tubes of 50ml for only 25 Euro
|
Catalog No |
Description |
Pcs/Bag |
Bag/box |
Total of boxes |
EUR/box |
EUR/10 boxes |
|
5540400 |
15 ml TC tubes, Round, PP (560 Units/box) |
40 |
14 |
787 |
35 |
250 |
|
5530300 |
TC Test Plate, 24 wells (85 Units/box) |
1 |
85 |
229 |
35 |
250 |
|
5540150 |
50 ml TC Tubes, Conical (440 Units/box) |
42 |
20 |
269 |
35 |
250 |
|
5530100 |
TC Orplate Test Plate, 96 Flat, 1 Unit/bag, 115 Units/box |
1 |
115 |
37 |
35 |
250 |
|
5530400 |
Tissue Culture Testplates 12 wells |
1 |
300 |
3 |
45 |
ASK |
TARGATT Fast & Efficient Gene Modification in Human Cells. Make your cell line in 3 months!
-
- Efficient insertion of any gene
-
- Single-copy
-
- Stable expression
- Click here to see all products
Based on our proprietary site-specific TARGATT technology for fast knock-in mouse (KI) generation, Applied StemCell Inc. (ASC) recently developed a TARGATT system specifically for introducing gene(s) of interest into mammalian cell lines. The unique advantage of this system is that any gene of interest can be inserted efficiently into a defined, transcriptionally-active locus with high gene expression in a single copy fashion.
Technical Details:
TARGATT technology enables highly efficient site-specific gene integration in mammalian cells and animals1, 2. This technology uses ?C31 integrase to insert any gene of interest into a docking site, pre-engineered in an intergenic region and transcriptionally active genomic locus. Our TARGATT technology improves several aspects in the generation of transgenic cell lines and animals: (1) High integration efficiency mediated by ?C31 integrase reduces time and cost; (2) Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high level expression of the transgene; (3) Integration at intergenic region ensures that no internal genes are interrupted; (4) Single copy gene integration eliminates repeat-induced gene silencing and genomic instability; (5) Site-specific integration allows a precise comparison of the effects of the transgenes among different lines. TARGATT technology can be utilized for a variety of applications including reporter gene expression, gene knockdown, disease cell and animal models.
More information - PDF presentation
