Suppliers
Contact Us
 GENTAUR Europe BVBAVoortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45 Fax 0032 16 50 90 45 This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it. |
GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280
Fax 0035929830072
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GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50
Fax 01 43 25 01 60
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GmbH Marienbongard 20
52062 Aachen Deutschland
Tel (+49) 0241 56 00 99 68
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GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
Tel 020 3393 8531
Fax 020 8445 9411
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GENTAUR Poland Sp. z o.o.
ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
Tel 058 710 33 44
Fax 058 710 33 48
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GENTAUR Nederland BV
Kuiper 1
5521 DG Eersel Nederland
Tel 0208-080893
Fax 0497-517897
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GENTAUR SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6, 24122 Bergamo
Tel 02 36 00 65 93
Fax 02 36 00 65 94
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GENTAUR Spain
Tel 0911876558
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Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
Phone/Fax:
(408) 780-0908
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GENPRICE Inc. invoicing/ accounting:
6017 Snell Ave, Suite 357
San Jose, CA. 96123
Serbia, 
Macedonia,
Montenegro, 
Croatia:
Tel 0035929830070
Fax 0035929830072
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GENTAUR Romania
Tel 0035929830070
Fax 0035929830072
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GENTAUR Greece
Tel 00302111768494
Fax 0032 16 50 90 45
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Other countries
Luxembourg +35220880274
Schweiz Züri +41435006251
Danmark +4569918806
Österreich +43720880899
Ceská republika Praha +420246019719
Ireland Dublin +35316526556
Norge Oslo +4721031366
Finland Helsset +358942419041
Sverige Stockholm +46852503438
Magyarország Budapest +3619980547
Better than mTeSR
Pluri-EZ™ hESC/iPSC Culture Medium
Cat #: ASM-5014
Product Size: 100 ml
Background: The ability to culture human embryonic stem cells (hESCs) and Induced Plurpotent Stem Cells (iPSCs) under chemically defined conditions is a prerequisite not only for the production of hESCs/iPSCs under GMP conditions but, also the development of protocols that will be used for future preclinical/clinical studies (1). Attempts at the formulation of chemically defined tissue culture conditions have included: 1) The replacement of serum in the medium with a chemically defined substitute (2,3) and the replacement of a MEF feeder layer with a defined feeder free culture surface (4-6).
Figure 1: (a) iPSC passage 5 growth curves comparing Pluri-EZ™ to mTeSR™. No statistical difference was found in iPSC growth. Typical iPSC colony grown using Pluri-EZ™(b) and mTeSR™(c).
Product description: Pluri-EZ is chemical based media and highly stable.
Storage conditions: -20˚C; 1 year, 4˚C; 2 months. Minimize exposure to direct light.
Shipping: On dry ice
Notes:
1. Thaw the Pluri-EZ™ medium either overnight at 4˚C or for 20 minutes at RT.
2. Addition of bFGF and Activin A may help cell culture results.
Neuro-Sure™ Neural Crest Stem Cell Culture Media
Cat #: ASM-4021
Product Size: 100 ml
Background: One of the defining characteristics of vertebrate development is the migration of neural crest cells from the neural tube. Neural crest cells give rise to a variety of tissues such as melanocytes, some heart vessels, cephalic neuroendocrine organs, connective tissue of the craniofacial skeleton and the neurons and glia of both the sensory and autonomic ganglia of the peripheral nervous systems 1,2. The study of neural stem cells and their progenitors, Neural Crest Stem Cells (NCSCs), have direct medical implications. Numerous pathologies, such as skeletal and nervous system disorders and peripheral neuropathies, arise from aberrant migration, specification or differentiation of neural crest cells3,4. In order to assist in your neural crest cell studies, our NCSC media has been optimized to contain all the factors required to support NCSCs plated on fibronectin coated tissue culture surfaces.
Product description and usage: Add 2 mL of NCSC medium supplement to 498 mL of
NCSC culture medium to produce 500 mL of NCSC media.
Storage conditions for NCSC medium: -80˚C; 1 year, 4˚C; two weeks. Minimize exposure
to direct light.
Storage condition for NCSC supplement: -80˚C; 1 year, 4˚C; two weeks.
Storage condition for NCSC media: 4˚C; two weeks. Minimize exposure to direct light.
Shipping: On dry ice.
Recommended procedure:
1. Thaw NCSC medium either overnight at 4˚C or for several hours at RT.
2. Thaw the NCSC medium supplement at RT.
3. Using sterile technique, add the NCSC medium supplement to the NSCS medium. Mix thoroughly and store the newly made media at 4˚C for up to two weeks. NOTE: NCSC medium can be filter sterilized if desired. Do NOT filter sterilize the NCSC medium supplement. Add this supplement directly to the NCSC medium.
4. Prior to use, warm the media to RT in the tissue culture hood. Due to the complex composition of the NCSC media, we do not recommend warming the media in a 370C water bath.
ESC-Sure™ Serum-/Feeder- Free hESC/iPSC Culture Medium (SFFM)
Cat #: ASM-5010
Product Size: 100 ml
Our Serum, feeder-free medium formulations are optimized for human ESC and iPSC culture. It contains growth factors and extracellular matrix proteins secreted by MEF cells.
SFFM is ready-to-use.
- Only need to add FGF2
- Culture dish coating: matrigel or similar matrix.
Blastocyst-Sure™ KSOM Embryo Culture Medium, with Phenol Red (with Amino Acid)
Cat #: ASM-5023
Product Size: 1 Pack (8 ml X 3 vials)
Size: 1 Pack (8 mL X 3 vials)
Blastocyst-Sure™ embryo culture medium is designed for in vitro culture of mouse embryos. Our optimized formula ensures that mouse embryos develop to blastocyst stage as close as they do in vivo. Each batch of Blastocyst-Sure™ embryo culture medium passes a stringent embryo culture test before they are released.
Formulation: Frozen liquid
Recommended protocol:
1. After thawing, the Blastocyst-Sure™ embryo culture medium should be used within 2 weeks. If you need to store media for later use, store it at 4C in dark for up to 2 weeks. Avoid repeated freeze/thaw cycles.
2. Prepare drops of Blastocyst-Sure™ embryo culture medium in advance. Cover medium drops with light mineral oil (embryo tested grade) and equilibrate in a 37C, 5% CO2 incubator for a couple of hours before use.
3. Change to fresh medium every 24h during embryo culture.
Blastocyst-Sure™ KSOM Embryo Culture Medium, without Phenol Red (with Amino Acid)
Cat #: ASM-5024
Product Size: 1 Pack (8 ml X 3 vials)
Size: 1 Pack (8 mL X 3 vials)
Blastocyst-Sure™ embryo culture medium is designed for in vitro culture of mouse embryos. Our optimized formula ensures that mouse embryos develop to blastocyst stage as close as they do in vivo. Each batch of Blastocyst-Sure™ embryo culture medium passes a stringent embryo culture test before they are released.
Formulation: Frozen liquid
Recommended protocol:
1. After thawing, the Blastocyst-Sure™ embryo culture medium should be used within 2 weeks. If you need to store media for later use, store it at 4C in dark for up to 2 weeks. Avoid repeated freeze/thaw cycles.
2. Prepare drops of Blastocyst-Sure™ embryo culture medium in advance. Cover medium drops with light mineral oil (embryo tested grade) and equilibrate in a 37C, 5% CO2 incubator for a couple of hours before use.
3. Change to fresh medium every 24h during embryo culture.
TALEN Assembly Kit
TALEN Assembly Kit
Simple (only a single-step), Fast (only 1 day), Cost-efficient !
TALEN (Transcription Activator-Like Effector Nuclease) is a novel gene targeting technology, which can solve the problem of low efficiency of RNAi and make gene targeting more convenient and efficient compared to the disadvantages of Zinc finger Nuclease Technology (ZFN) which is high cost, complicated screening, high cell toxicity and low-efficiency.
Features of our kit：
Simple: only a single step of ligation and transformation; can be handled by anyone with basic molecular biology technology.
Fast: accomplish TALEN construction within 1 day; obtain sequenced TALEN vector within 3 days.
Cost-efficient: the cost of each TALEN vector.
PS：Patent application pending.
Successful species：
Animal：human, rat, mouse, zebrafish, rabbit, Drosophila, porcine, goat, rabbit, bombyx, and Xenopus
Plant：Oryza sativa, and arabidopsis
Germ：Yeast
For more information download our flyerInstructional Movies for the Bullet Blender Homogenizer
1. Guidelines for using the Bullet Blender Homogenizer
Neural Crest Stem Cell Culture medium + Supplement
a) NCSCs forming characteristic “lacunae” during
proliferation on ASC’s NCSC media (10X)
b) NCSCs at a cell density ready for passaging.(20X)
Background: One of the defining characteristics of vertebrate development is the migration of neural crest cells from the neural tube. Neural crest cells give rise to a variety of tissues such as melanocytes, some heart vessels, cephalic neuroendocrine organs, connective tissue of the craniofacial skeleton and the neurons and glia of both the sensory and autonomic ganglia of the peripheral nervous systems. The study of neural stem cells and their progenitors, Neural Crest Stem Cells (NCSCs), have direct medical implications. Numerous pathologies, such as skeletal and nervous system disorders and peripheral neuropathies, arise from aberrant migration, specification or differentiation of neural crest cells. In order to assist in your neural crest cell studies, our NCSC media has been optimized to contain all the factors required to support NCSCs plated on fibronectin coated tissue culture surfaces.
Product description: Add 2 mL of NCSC medium supplement to 498 mL of NCSC medium to produce 500 mL of NCSC media.
Storage conditions for NCSC medium: -80°C; 1 year, 4°C; two weeks. Minimize exposure to direct light.
Storage condition for NCSC supplement: -80°C; 1 year, 4°C; two weeks.
Storage condition for NCSC media: -80°C; 1 year, 4°C; two weeks. Minimize exposure to direct light.
Shipping: On dry ice.
Recommended procedure:
1. Thaw NCSC medium either overnight at 4°C or for several hours at RT.
2. Thaw the NCSC medium supplement at RT.
3. Using sterile technique, add the NCSC medium supplement to the NSCS medium. Mix thoroughly and store the newly made media at 4°C for up to two weeks. NOTE: NCSC medium can be filter sterilized if desired. Do NOT filter sterilize the NCSC medium supplement. Add this supplement directly to the NCSC medium.
4. Prior to use, warm the media to RT in the tissue culture hood. Due to the complex composition of the NCSC media, we do not recommend warming the media in a 37 C water bath.
Price: 80 EUR
hESC-Sure Serum- and Feeder-Free Medium(SFFM)
Above: hESC in ESC-Sure
conditioned medium
Bottom: hESC on MEF TM
Background: Traditionally human embryonic stem cell (hESC) culture requires a mouse embryonic fibroblast (MEF) cells’ support, which makes the protocol complicated. Our Serum- and Feeder-free medium that is for human ESC culture contains all the growth factors needed in hESC culture, except the bFGF; eliminate the routine preparation of feeder. It is a ready-to-use product for a serum/feeder-free system. Our medium makes your cell culture more efficient and easier to control.
Serum- and Feeder-free medium (1x) for proliferation of pluripotent human embryonic stem cell (hESC) culture in a serum/feeder-free system. Each and every batch has been tested for hES/iPS cells pluripotency. All you need to add is 20 ng/ml bFGF.
Product description: 100 mL of hESC-Sure
Source: Chemical plus growth factors
Storage: -80°C
Shipping: Shipped on dry ice.
Recommended procedure:
Human ESC culture procedure using hESC-Sure Serum- and Feeder-free medium:
1. Coat culture dish with Matrigel the day before.
2. Wash Matrigel (BD Biosciences Cat# 354277) coat dishes with DMEM/F12 medium(Targatt Cat# ASM-5002).
3. Once human ES cell were confluent or cultured for more than 5 days, disaggregated cells
with 1mg/ml Dispase(Dilute 5mg/ml stock solution with DMEM/F12 media), incubate at 37
C for 3 minutes.
4. Wash container with 1x PBS, and to scrape colonies off the bottom of the container with cell
scraper.
5. Collect the cells in the 50ml or 15ml falcon tube, pellet by centrifugation at 700rpm for 5 min
at room temperature.
6. Resuspend hESC pellets in hESC-Sure Serum- and Feeder-free culture medium supplemented with 20 ng/ml bFGF.
TM
7. Split cells at 1:3 to 1:5 ratio every 4 to 5 days using 1mg/ml Dispase (diluted with
DMEM/F12).
8. 1x freezing medium contains 90% knockout serum and 10% DMSO.
Price: 104 EUR
Targatt transgenic Kit
TARGATT Transgenic Kit is designed to create site-specific, knock-in transgenic mice in a more efficient and significantly faster way over traditional methods. Generating transgenic mice by conventional methods (e.g. pronuclear microinjection or lentiviral injection) has several limitations, first of which is random insertion of the transgene. Random insertion of a transgene results in a position effect where either the transgene is prone to silencing or endogenous gene expression is disrupted. Furthermore, this position effect is compounded when a transgene is inserted as multiple copies, resulting in instability at the insertion locus.
Using our proprietary site-specific DNA integration system, TARGATT Transgenic Kit, combined with our genetically TARGATT mouse embryos (cat.# AST-0001, AST-0002, AST-0003, AST-0004). Targatt offers high quality H11(Hipp11) and ROSA26 transgenic mouse kits with our TARGATT technology. Using our TARGATT Technology, you can generate your own knock-in mouse in just three months.
If you prefer to generate a knock-in mouse model on your own, TARGATT Embryo H11(Hipp11) or Rosa26 and Transgenic Kits (2 or 5 microinjection size) are available for purchase.
Background:
TARGATT technology enables highly efficient site-specific gene integration in mammalian cells and animals1, 2. This technology uses øC31 integrase to insert any gene of interest into a docking site, pre-engineered in an intergenic region and transcriptionally active genomic locus. Our TARGATT technology improves several aspects in the generation of transgenic cell lines and animals: (1) High integration efficiency mediated by øC31 integrase reduces time and cost; (2) Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high level expression of the transgene; (3) Integration at intergenic region ensures that no internal genes are interrupted; (4) Single copy gene integration eliminates repeat-induced gene silencing and genomic instability; (5) Site-specific integration allows a precise comparison of the effects of the transgenes among different lines. TARGATT technology can be utilized for a variety of applications including reporter gene expression, gene knockdown, disease cell and animal models.
TARGATT embryos
TARGATT embryos are derived from one of our genetically engineered mouse models. These mouse embryos can be used as embryo donors for creating site-specific transgenic mice in a more efficient and faster way compared to traditional methods. Generating transgenic mice by conventional methods (e.g. pronuclear microinjection or lentiviral injection) has several limitations, first of which is the random insertion of the transgene. Random insertion of a transgene results in a position effect where either the transgene is prone to silencing or endogenous gene expression is disrupted. Furthermore, this position effect is compounded when transgenes are inserted in multiple copies, resulting in genomic instability at the insertion locus. Using our proprietary site-specific DNA integration technology, TARGATT embryos combined with our TARGATT Transgenic Kit (cat# AST-1001 or AST-1002), you can generate your desired transgenic mouse models with guaranteed gene expression faster.
Gentaur offers high quality H11 and ROSA26 TARGATT frozen embryos. Using our TARGATT Technology, you can generate your own knock-in mouse from H11 or ROSA26 embryos in 3 months. Our H11(Hipp11) and ROSA26 frozen embryos contain 3 straws with a total 45-60 cryopreserved embryos (8-cell stage/morula). They are shipped in a dry shipper containing liquid nitrogen. We also offer fresh H11 and ROSA26 embryos with each vial containing 25-35 fresh embryos (E3.5) in KSOM medium. We offer overnight shipping at ambient temperature. Embryos will be transferred to recipient(s) immediately upon arrival. US customers only.
If you prefer to generate a knock-in mouse model on your own, TARGATT Embryo (H11 or Rosa26) and Transgenic Kits (2 or 5 microinjection size) are available for purchase.
Background:
TARGATT technology enables highly efficient site-specific gene integration in mammalian cells and animals1, 2. This technology uses øC31 integrase to insert any gene of interest into a docking site, pre-engineered in an intergenic region and transcriptionally active genomic locus. Our TARGATT technology improves several aspects in the generation of transgenic cell lines and animals: (1) High integration efficiency mediated by øC31 integrase reduces time and cost; (2) Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high level expression of the transgene; (3) Integration at intergenic region ensures that no internal genes are interrupted; (4) Single copy gene integration eliminates repeat-induced gene silencing and genomic instability; (5) Site-specific integration allows a precise comparison of the effects of the transgenes among different lines. TARGATT technology can be utilized for a variety of applications including reporter gene expression, gene knockdown, disease cell and animal models.
TARGATT Knockin Mice - Stem Cells Products
TARGATT Embryos
Using our novel TARGATT system, a gene of interest can be specifically inserted at a well-characterized, transcriptionally-active locus in the mouse genome with guaranteed transgene expression. Tissue-specific and / or ubiquitous expression options are available.
Advantages of TARGATT technology:
- Site-specific gene integration at a transcriptionally-active locus ensures high-level gene expression.
- Integration happens at an intergenic region; no internal genes are disrupted.
- The integrase system catalyzes a unidirectional integration event and results in a high efficiency in producing transgenic mice.
- Gene integration at the same locus allows a precise comparison of the transgenics from one line to another.
TARGATT Kits
TARGATT Supporting Materials
Mouse Embryonic Fibroblasts (MEF)
● CF-1
● Neo-resistant
● DR4
● SNL (STO feeder cells)
SNL (STO feeder cells)
Cell Culture Products
● Germline-tested & ESC-qualified FBS
● Specialty Media
● Basal Media (DMEM)
● Stem Cell Growth and Differentiation Factors
● ASC Small Molecules
● 3D Culture and Expansion System
Reprogramming
ESC/iPSC Characterization
Pluripotency Protein Markers Stem Cell Gene Array
● Pluripotency mRNA Markers
● Components
ESC/iPSC Differentiation
● Neural Differentiation
● Dendritic Cell (DC) Generation
ES/iPS Cell Lines
Mouse ES Cell Lines Human iPS Cells
Cell Depository
Primary Cell, cDNA, RNA (Normal)
● Endocrine
● Neural
● Pulmonary
● Digestive
● Urinary
● Reproductive
● Skeletal Muscle
● Hematopoietic
● Integumentary
● Cardiovascular
● Miscellaneous
Primary Cell, cDNA, RNA (Disease)
● Blood Disorders
● Neurological Disorders
● Degenerative Disorders
● Metabolic Disorders
● Cardiovascular Disorders
● Congenital Disorders
● Endocrine Disorders
● Autoimmune Disorders
● Genetic Disorders
● Muscular Disorders
● Oncogenic Disorders
AccuPower DNA Ligation PreMix
The AccuPower DNA Ligation PreMix is a lyophilized master mix containing T4 DNA Ligase, ATP, reaction buffer, and patented stabilizer. This DNA ligation premix is conveniently aliquoted in strip-tubes for reactions; you need only add DNAs to be ligated and water. The reaction will work for DNA ligation for all applications: blunt cloning, sticky end cloning and TA cloning. The premix is stable up to four months at room temperature and for three years at -20°C.
Features and Benefits
| Ready to use premix: | Minimal set up time and handling required |
| Fast: | Only 5 minutes for cohesive-end ligation and 10 minutes for blunt-end ligation at room temperature |
| Stable: | Enzyme activity for up to four months at room temperature and for three years in the freezer |
Application
Cloning into vectors, library construction, TA cloning, linker ligation, and re-circlization of linear DNA
Figure 1. Stability test of AccuPower DNA Ligation PreMix at room temperature.
Lane 1 – 12: Lambda DNA / Hind lll fragment (1 µg)
Lane 12 – 24: Lambda DNA / EcoR V fragment (1 µg)
Lane 2, 3, 14, 15: Ligation with AccuPower Ligation PreMix stored at room temperature for 1 month
Lane 5, 6, 17, 18: Ligation with AccuPower Ligation PreMix stored at room temperature for 2 months
Lane 8, 9, 20, 21: Ligation with AccuPower Ligation PreMix stored at room temperature for 3 months
Lane 11, 12, 23, 24: Ligation with AccuPower Ligation PreMix stored at room temperature for 4 months
Figure 2. DNA Ligation efficiency comparison between AccuPower DNA Ligation PreMix and other competitors’ products.
Lane 1, 9: Intact Lambda DNA (1 µg)
Lane 2 – 8: Lambda DNA / Hind lll fragment (1 µg)
Lane 10 – 16: Lambda DNA / EcoR V fragment (1 µg)
Lane 3, 4, 11, 12: Ligation with AccuPower Ligation PreMix
Lane 5, 13: Ligation with T4 DNA Ligase from company N
Lane 6, 14: Ligation with Quick Ligation Kit from company N
Lane 7, 15: Ligation with LigFast Rapid DNA Ligation System from company P
Lane 8, 16: Ligation with Ready-To-Go T4 DNA Ligase from company A
AccuPower DualStar qPCR PreMix
AccuPower DualStar qPCR PreMix is a lyophilized PCR master mix containing a thermostable DNA polymerase, thermostable pyrophosphatase, reaction buffer, dNTPs, tracking dye, and patented stabilizer in a ready to use hotstart qPCR master mix.
DualStar qPCR PreMix eliminates nonspecific reaction, while providing high sensitivity, due to our novel HotStart methodology. Gentaur uses a unique enzyme-mediated HotStart PCR that provides robust and reproducible results. Gentaur’s Top DNA Polymerase is inhibited at lower temperatures (< 70°C) by pyrophosphate. However, Top DNA Polymerase is rendered fully active at temperatures above 70°C via pyrophosphate hydrolysis with our thermostable pyrophosphatase. This prevents the formation of mis-primed products and primer-dimers during the reaction set up process resulting in improved PCR specificity. It is ideal for nucleic acid amplification reactions involving complex genomic or cDNA templates, very low copy targets, and multiplex reactions.
DualStar Plus qPCR PreMix goes a step further by providing resistance to common inhibitors of PCR (Blood-EDTA, Hemoglobin, Humic Acid from various sources, etc.) providing you with robust and reliable results, even with poor quality samples. Tested for resistance against many common inhibitors of PCR, DualStar plus is the enzyme of choice for any PCR that you want to work right – the first time and every time!
Features and Benefits
| Ease of use: | Just add template and primers and start your PCR. dNTPs, buffer and enzyme are provided. |
| Stability: | Stable at room temperature for a month and for 2 years in a -20°C freezer |
| HotStart: | Unique enzyme mediated HotStart results in greater specificity and more robust reactions |
| Reproducibility: | Each batch is produced under strict ISO quality controls. |
| DualStar Plus qPCR PreMix: | Available for less samples that will not work in other systems due to low purity |
Specifications
5' to 3' exonuclease activity: Yes
3' to 5' exonuclease activity: No
Terminal transferase activity: Yes
Fragment Size: 1 kb
Application
- Gene expression profiling
- Target DNA quantification
- Microbial detection
- SNP analysis
- Viral/bacterial pathogen load determination
- Evaluation of primer pair performance for probe-based real-time PCR
Figure 1. Real-Time PCR Data of AccuPower® DualStar™ qPCR PreMix
AccuPower® DualStar™ qPCR PreMix provides at least 7 orders of magnitude in dynamic range (107 ~ 101copies/reaction).
A: Amplification curve. West Nile Virus (WNV) primers and TaqMan-based probe were added into DualStar qPCR Premix. A series of WNV positive control diluents were tested.
B: Standard curve. All data were obtained using Exicycler™ 96 Real-Time Quantitative PCR System (Gentaur).
Figure 2. Data using Various kinds of Real-time PCR Instruments
AccuPower® DualStar™ qPCR PreMix provides at least 7 orders of magnitude in dynamic range (107 ~ 101copies/reaction).
A: Amplification curve. and standard curve using ABI 7500 Format Fast Real-time PCR machine (Applied Biosystems). A series of WNV positive control diluents were tested.
B: Amplification curve and standard curve using ABI 7500 Format Real-time PCR machine (Applied Biosystems).
C: Amplification curve and standard curve using Opticon Real-time PCR machine (MJ Research, currently Bio-Rad).
Figure 3. Comparison of detection sensitivity between AccuPower® DualStar™ qPCR PreMix and other supplier's master mixture
West Nile Virus (WNV) primers and TaqMan-based probe were added into AccuPower® DualStar™ qPCR Premix and other supplier's master mixture. A series of WNV positive control diluents were tested. Reaction mixtures were prepared and qPCR was performed according to each supplier's protocol. All data were obtained using ABI 7500 Format Fast Real-time PCR machine (Applied Biosystems).
