Contact Us

GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
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    GENTAUR France

     GENTAUR France SARL
    9, rue Lagrange, 75005 Paris 
    Tel 01 43 25 01 50 
    Fax 01 43 25 01 60
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    Gentaur Germany

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      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
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    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
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    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
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    GENTAUR Nederland

     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
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    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
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    GENTAUR Spain

     GENTAUR Spain
    Tel 0911876558
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    Genprice USA

    usa-flagGenprice Inc, Logistics
    547, Yurok Circle
    San Jose, CA 95123
    Phone/Fax: 

    (408) 780-0908 

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    skype chat

    GENPRICE Inc. invoicing/ accounting:
    6017 Snell Ave, Suite 357
    San Jose, CA. 96123

     

    Gentaur Serbia

    serbiaSerbia, Macedonia FlagMacedonia, 

    montenegro-flagMontenegro, croatiaCroatia: 
    Tel 0035929830070 
    Fax 0035929830072
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    GENTAUR Romania

    romGENTAUR Romania

    Tel 0035929830070 
    Fax 0035929830072
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    GENTAUR Greece

    grGENTAUR Greece 

    Tel 00302111768494 
    Fax 0032 16 50 90 45

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    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
    Danmark +4569918806
    Österreich +43720880899
    Ceská republika Praha +420246019719
    Ireland Dublin +35316526556
    Norge Oslo +4721031366
    Finland Helsset +358942419041
    Sverige Stockholm +46852503438
    Magyarország Budapest +3619980547

    seal-in-search-symantec

     

     

    We are currently offering a comprehensive panel of antibodies specific for human Norovirus, including GII.4 2012 Sydney strain.  MAB223P through MAB228P have been tested across a broad range of strains, as reported in the table below.  In addition to being broadly cross reactive to GII.4 genotypes, MAB227P has demonstrated the ability to block ligand binding in surrogate neutralization assays.  MAB228P, developed against GI.1 1968 virus like particles (VLPs), has been shown to recognize additional GI types.  There are currently five known genogroups, of which three cause human disease: GI, GII, and GIV.  According to the CDC, since 2002 GII.4 has been the most common cause of Norovirus outbreaks.

    mono3

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    Monday, 01 July 2013 17:03

    RUNBLUE PRECAST GELS

    run blue gentaur gelsRunBlue precast gels have superior rigidity and stability over traditional polyacrlyamide gels. For your convenience we have already removed the comb. The cassette locks the fingers in place and there is no tape to be removed. 

    Long term storage of up to 24 months store at 4°C or for 3 months at room temperature. For expiry date see box. 

    We recommend using RunBlue LDS Sample Buffer 4x which has been specifically formulated for use with our gels. The ions in the sample buffer match the gel buffer and it has a higher density, making it compatible with the density of the running buffer.

    Heat the samples, reduced or non-reduced, for 10 minutes at 70°C. ƒ Reduced samples should be run within 2 hours to prevent re-oxidation. ƒ Maximum volume that can be loaded in the wells is 35 µl.

     

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    Wednesday, 26 June 2013 15:48

    GelRed Nucleic Acid Gel Stain In Water

    Gelred GelgreenGelRed™ is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed is far more sensitive than EB without requiring a destaining step (Figure 1). GelRed and EB have virtually the same spectra (Figure 3), so you can directly replace EB with GelRed without changing your existing imaging system.
    GelRed™ can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. GelRed can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. GelRed is also compatible with downstream DNA manipulations such as restriction digest, sequencing, and cloning.
    A series of safety tests have confirmed that GelRedTM is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal.
    We offer GelRed™ 10,000X solution in DMSO and in water (cat#41003) for better safety. For your convenience, we also offer ready-to-use GelRedTM 3X in water (cat#41001) that can be directly used for post gel staining. For customers who look for large pack size, we offer a cost-saving bulk pack size of 10mL (cat#41003-1).

    FEATURES:
    Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
    Passed environmental safety tests for direct disposal down the drain or in regular trash
    Much more sensitive than EtBr and SYBR Safe
    Available in water, stable at room temperature for long-term storage and microwavable
    - Safer than EB
    - Easy disposal
    - Ultra-sensitive
    - Extremely stable

    Very simple procedures for either precast and post gel staining
    GelRed replaces EtBr with no optical setting change; GelGreen replaces SYBR or GelStar with no optical setting change (see Figure 3)
    Compatible with downstream DNA manipulations such as restriction digest, sequencing and cloning.
    Simple to use
    Perfectly compatible with a standard UV transilluminator
    Perfectly compatible with downstream applications

    gelred3gelred4Figure 1.GelRed™ is significantly more sensitive than ethidium bromide (EB) for detecting low-level DNA, especially in the lower molecular weight area. Shown left are two-fold serial dilutions of 1 Kb Plus DNA Ladder from Invitrogen electrophoresed on 1% agarose gels precasted with GelRed or EB in 1x TBE. The total amount of DNA loaded per lane was: 200 ng, 100 ng, 50 ng and 25 ng from left to right. Gels were imaged using 300-nm transillumination and photographed with an EB filter and Polaroid 667 black-and-white print films.

     

     

    The Most Sensitive and Stable Precast Gel Stain

     

    gelred f2

    Figure 2. GelRed™ displays consistently superior sensitivity for post gel staining, regardless of the filter used (A vs. C) and storage and handling condition. SYBR Gold, however, showed comparable performance only when used fresh from the manufacturer and with a SYBR filter (B vs. D). Following a few freeze-thaw cycles, SYBR Gold 10,000X solution degraded significantly, resulting in poor staining (E). SYBR Gold 1X solution also degrades over time (see Figure 4). Two-fold serial dilutions of 1kb Plus DNA Ladder from Invitrogen were electrophoresed on 1% agarose gels in 1x TBE and post- stained with GelRedTMand SYBR Gold, respectively. Gels were imaged using 300-nm transillumination and photographed with the indicated filters and Polaroid black-and-white print films. The total amount of DNA per lane for each serial dilution was: 200 ng, 100 ng, 50 ng and 25 ng from left to right.

    gelred f3

    Figure 3. Excitation and emission spectra of GelRed and GelGreen in the presence of DNA in PBS buffer

    gelred f4

    Note: *GelRed and its uses are covered by pending US and international patents. **SYBR is trademark of Molecular Probes, Inc. and GelStar is trademark of FMC corporation.
    Please also see our EvaGreen™(cat#31000), a breakthrough nucleic acid dye ideally suited for quantitative real-time PCR(qPCR). By incorporating a smart "release-on-demand" DNA-binding technology, EvaGreen™ has low PCR inhibition while exhibiting superior sensitivity. Similar to our GelRed™, EvaGreenTM has remarkable stability.
    Reference:
    For Electrophoretic Mobility Shift Assay: 1. Liu,Y.,et al. Biochemistry, DOI: 10.1021/bi902050p, 2010, 2. Konate, K., et al. Biochemistry, DOI: 10.1021/bi901791x, 2010.

    Catalog number : 41003

    Quantity: 0.5

    Price: 180 Euro

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    The 25-Hydroxyvitamin D Total ELISA is powered by unique monoclonal antibodies that express 100 % reactivity against 25 OH Vitamin D3 and 83% reactivity against 25OH Vitamin D2.
    All In One technology. No extraction step, displacement solution directly into the well. Results in less than 4 hours. Calibrated against ID-LC/MS-MS (reference method). Bar codes on all components.

    The  25-Hydroxyvitamin D Total ELISA

     

    Cat#

    Description

    Kit

    Quantity

    Price

    GDMS 1971

    25OH Vitamin D Total ELISA

    96T

    1-10

    € 180

    GDMS 1971

    25OH Vitamin D Total ELISA

    96T

    11-50

    € 155

    GDMS 1971

    25OH Vitamin D Total ELISA

    96T

    > 50

    € 145

     

    Technical spec.:

    Catalog # GDMS 1971
    Format ELISA MT
    Total Incubation Time < 4 hours
    Size 96 tests
    Sample Type Serum
    Sample Volume 50 µL
    Controls 2 levels
    Range 0-180 ng/mL
    Sensitivity 1,4 ng/mL
    Protocol steps 120min./30 min./15 min
    Wavelength 450 nm

     

    Downloads:

    PDF-IconProtocol comparison with ELISA

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    Monday, 24 June 2013 15:06

    New Purified Genomic DNA Products

    Vibrio cholerae Z132, DNA (10 µg)

    PRODUCT DESCRIPTION: Each aliquot contains 10 µg of DNA extracted from a pure culture of Vibrio cholerae. The identification of this organism was confirmed by 16S sequencing. The purity of the culture was monitored by Gram staining and by additional culturing. The DNA was extracted from the cells following the bacterial protocol from the Qiagen®Genomic DNA Handbook using Qiagen®Genomic DNA Buffers with a 500/G genomic tip.DNA concentration and A260/280 ratios are determined using a NanoDrop ND-1000®. The extracted DNA also tested positive on an inhouse real time PCR assay.

    INTENDED USE: Purified Genomic DNA is designed for use as an amplification and/or detection control for nucleic acid testing of Vibrio cholerae. It can also be used to determine a limit of detection (LOD), in diagnostic assay development, cross-reactivity studies or genomic sequencing. When used as a control for nucleic acid tests, the same protocols as those used to amplify extracted clinical specimens should be employed.

    PRECAUTIONS:
    - Use Universal Precautions when handling Genomic DNA.
    - The material may be re-frozen after thawing. Repetitive freezing and thawing is not recommended (aliquot material if necessary).
    - To avoid cross-contamination, use separate pipette tips for all reagents.

    RECOMMENDED STORAGE: This control is supplied in TE Buffer and should be frozen at -20°C or below.

    DO NOT USE IN HUMANS: These products are intended for research, product development or manufacturing use only. These products are NOT intended for use in the manufacture or processing of injectable products subject to licensure under section 351 of the Public Health Service Act or for any other product intended for administration to humans.

    Escherichia coli O111:NM, DNA (10 µg)

    PRODUCT DESCRIPTION: Each aliquot contains 10 µg of DNA extracted from a pure culture of Escherichia coli. The identification of this organism was confirmed by 16S sequencing. The purity of the culture was monitored by Gram staining and by additional culturing. he DNA was extracted from the cells following the bacterial protocol from the Qiagen®Genomic DNA Handbook using Qiagen®Genomic DNA Buffers with a 500/G genomic tip.DNA concentration and A260/280 ratios are determined using a NanoDrop ND-1000®. The extracted DNA also tested positive on an inhouse real time PCR assay.

    INTENDED USE: Purified Genomic DNA is designed for use as an amplification and/or detection control for nucleic acid testing of Escherichia coli. It can also be used to determine a limit of detection (LOD), in diagnostic assay development, cross-reactivity studies or enomic sequencing. When used as a control for nucleic acid tests, the same protocols as those used to amplify extracted clinical specimens should be employed.

    PRECAUTIONS:
    - Use Universal Precautions when handling Genomic DNA.
    - The material may be re-frozen after thawing. Repetitive freezing and thawing is not recommended (aliquot material if necessary).
    - To avoid cross-contamination, use separate pipette tips for all reagents.

    RECOMMENDED STORAGE: This control is supplied in TE Buffer and should be frozen at -20°C or below.

    Plesiomonas shigelloides Z130, DNA (10 µg)

    PRODUCT DESCRIPTION: Each aliquot contains 10 µg of DNA extracted from a pure culture of Plesiomonas shigelloides. The identification of this organism was confirmed by 16S sequencing. The purity of the culture was monitored by Gram staining and by dditional culturing. The DNA was extracted from the cells following the bacterial protocol from the Qiagen® Genomic DNA Handbook using Qiagen® Genomic DNA Buffers with a 500/G genomic tip. DNA concentration and A260/280 ratios are determined using a NanoDrop ND-1000®. The extracted DNA also tested positive on an inhouse real time PCR assay.

    INTENDED USE: Purified Genomic DNA is designed for use as an amplification and/or detection control for nucleic acid testing of Plesiomonas shigelloides. It can also be used to determine a limit of detection (LOD), in diagnostic assay development, crossreactivity tudies or genomic sequencing. When used as a control for nucleic acid tests, the same protocols as those used to amplify extracted clinical specimens should be employed.

    PRECAUTIONS:
    - Use Universal Precautions when handling Genomic DNA.
    - The material may be re-frozen after thawing. Repetitive freezing and thawing is not recommended (aliquot material if necessary).
    - To avoid cross-contamination, use separate pipette tips for all reagents.

    RECOMMENDED STORAGE: This control is supplied in TE Buffer and should be frozen at -20°C or below.

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    Gene silencing by RNA interference (RNAi) technology using small interfering RNA (siRNA) is a powerful tool for studying functional genomics, drug target identification and validation, pathway elucidation, and therapeutic development. The advantage is in the algorithm we use for siRNA design. Our Turbo si-Designer software identifies highly effective siRNA target sites with remarkably high siRNA knockdown rates. Critical parameters including base composition, thermodynamic instability and base preference are all considered in the design algorithm. siRNAs spanning SNP sites are removed and finally, non-specific siRNAs are eliminated following homology searching by BLAST and Smith-Waterman algorithms. The result is an siRNA with extraordinary siRNA knockdown efficiency and minimal off-target effects. The performance of Turbo si-Designer has been extensively evaluated by testing the siRNA knockdown efficiency of thousands of siRNAs targeting STE Kinases, TK Kinases, genes involved in the NF-kappaB and Caspase Pathways using Real-time PCR analysis. The results of the evaluation indicated the design algorithm was highly effective in selecting effective siRNAs; 80% of the tested siRNAs showed > 70% knockdown and 38% elicited knockdown of > 90%.

     

    Cat. No. Guaranteed Yield Purification
    S-1017-5 1 nmole BioRP
    S-1017-6 5 nmole
    S-1017-1 10 nmole
    S-1017-2 20 nmole
    S-1017-3 50 nmole
    S-1017-4 100 nmole
    S-1018-5 1 nmole HPLC
    S-1018-6 5 nmole
    S-1018-1 10 nmole
    S-1018-2 20 nmole
    S-1018-3 50 nmole
    S-1018-4 100 nmole

     

    - siRNA provided lyophilized and annealed.
    - Annealing buffer is provided when single-stranded siRNA requested.

     

     

    Friday, 21 June 2013 15:30

    AccuTarget Control siRNAs

     

    AccuTarget™ Positive Control siRNAs

    Cat. No. Product Description Purification Guaranteed Yield
    SP-1001 AccuTarget™ GAPDH Positive Control siRNA BioRP 5 nmole
    SP-1002 AccuTarget™ GAPDH Positive Control siRNA BioRP 10 nmole
    SP-1003 AccuTarget™ GAPDH Positive Control siRNA BioRP 20 nmole
    SP-1011 AccuTarget™ GAPDH Positive Control siRNA HPLC 5 nmole
    SP-1012 AccuTarget™ GAPDH Positive Control siRNA HPLC 10 nmole
    SP-1013 AccuTarget™ GAPDH Positive Control siRNA HPLC 20 nmole
    SP-2001 AccuTarget™ GFP Positive Control siRNA BioRP 5 nmole
    SP-2002 AccuTarget™ GFP Positive Control siRNA BioRP 10 nmole
    SP-2003 AccuTarget™ GFP Positive Control siRNA BioRP 20 nmole
    SP-2011 AccuTarget™ GFP Positive Control siRNA HPLC 5 nmole
    SP-2012 AccuTarget™ GFP Positive Control siRNA HPLC 10 nmole
    SP-2013 AccuTarget™ GFP Positive Control siRNA HPLC 20 nmole
    SP-3001 AccuTarget™ Luciferase Positive Control siRNA BioRP 5 nmole
    SP-3002 AccuTarget™ Luciferase Positive Control siRNA BioRP 10 nmole
    SP-3003 AccuTarget™ Luciferase Positive Control siRNA BioRP 20 nmole
    SP-3011 AccuTarget™ Luciferase Positive Control siRNA HPLC 5 nmole
    SP-3012 AccuTarget™ Luciferase Positive Control siRNA HPLC 10 nmole
    SP-3013 AccuTarget™ Luciferase Positive Control siRNA HPLC 20 nmole



    AccuTarget™ Negative Control siRNAs

    Cat. No. Product Description Purification Guaranteed Yield
    SN-1001 AccuTarget™ Negative Contol siRNA BioRP 5 nmole
    SN-1002 AccuTarget™ Negative Contol siRNA BioRP 10 nmole
    SN-1003 AccuTarget™ Negative Contol siRNA BioRP 20 nmole
    SN-1011 AccuTarget™ Negative Contol siRNA HPLC 5 nmole
    SN-1012 AccuTarget™ Negative Contol siRNA HPLC 10 nmole
    SN-1013 AccuTarget™ Negative Contol siRNA HPLC 20 nmole
    SN-1021 AccuTarget™ Fluorescein-labeled Negative Control siRNA HPLC 5 nmole
    SN-1022 AccuTarget™ Fluorescein-labeled Negative Control siRNA HPLC 10 nmole
    SN-1023 AccuTarget™ Fluorescein-labeled Negative Control siRNA HPLC 20 nmole



    AccuTarget™ Control siRNA Sets

    Cat. No. Product Description Purification Guaranteed Yield
    SS-1001 AccuTarget™ GAPDH Control siRNA Set BioRP 5 nmole positive control
    + 2 nmole negative control
    SS-1002 AccuTarget™ GFP Control siRNA Set BioRP 5 nmole positive control
    + 2 nmole negative control
    SS-1003 AccuTarget™ Luciferase Control siRNA Set BioRP 5 nmole positive control
    + 2 nmole negative control
    SS-1011 AccuTarget™ GAPDH Control siRNA Set HPLC 5 nmole positive control
    + 2 nmole negative control
    SS-1012 AccuTarget™ GFP Control siRNA Set HPLC 5 nmole positive control
    + 2 nmole negative control
    SS-1013 AccuTarget™ Luciferase Control siRNA Set HPLC 5 nmole positive control
    + 2 nmole negative control
    Friday, 21 June 2013 15:20

    AccuTarget™ Premade siRNA Sets

     

    siRNA Set

    Cat. No. Product Description No. of Genes No. of siRNAs
    SHS-0010 AccuTarget™ Human Antioxidant siRNA Set 38 114
    SHS-0020 AccuTarget™ Human Apoptosis siRNA Set 290 870
    SHS-0250 AccuTarget™ Human Cancer siRNA set 1157 3471
    SHS-0030 AccuTarget™ Human Caspase siRNA set 37 111
    SHS-0040 AccuTarget™ Human Cell cycle siRNA Set 112 336
    SHS-0050 AccuTarget™ Human Cyclase siRNA Set 21 63
    SHS-0060 AccuTarget™ Human Cytochrome P450 siRNA Set 52 156
    SHS-0070 AccuTarget™ Human Deaminase siRNA Set 22 66
    SHS-0080 AccuTarget™ Human GPCR signaling pathway siRNA set 727 2181
    SHS-0090 AccuTarget™ Human Helicase siRNA Set 114 342
    SHS-0100 AccuTarget™ Human Isomerase siRNA Set 104 312
    SHS-0110 AccuTarget™ Human Kinase siRNA Set 699 2097
    SHS-0120 AccuTarget™ Human Ligase siRNA Set 272 816
    SHS-0130 AccuTarget™ Human Lyase siRNA Set 123 369
    SHS-0140 AccuTarget™ Human Motor siRNA Set 122 366
    SHS-0150 AccuTarget™ Human NF-kB pathway siRNA Set 37 111
    SHS-0160 AccuTarget™ Human Nucleic acid binding siRNA Set 2573 7719
    SHS-0170 AccuTarget™ Human Oxidoreductase siRNA Set 551 1653
    SHS-0180 AccuTarget™ Human Peptidase siRNA Set 491 1473
    SHS-0190 AccuTarget™ Human Phosphatase siRNA Set 188 564
    SHS-0200 AccuTarget™ Human Receptor siRNA Set 1516 4548
    SHS-0210 AccuTarget™ Human Transferase siRNA Set 1428 4284
    SHS-0220 AccuTarget™ Human Transporter siRNA Set 1021 3063
    SHS-0230 AccuTarget™ Human Tubulin siRNA Set 20 60
    SHS-0240 AccuTarget™ Human Ubiquitin siRNA Set 77 231



    siRNA Subset

    Cat. No. Product Description No. of Genes No. of siRNAs
    SHS-0110-1 AccuTarget™ Human AGC Kinase siRNA Subset 58 174
    SHS-0110-2 AccuTarget™ Human CAMK Kinase siRNA Subset 65 195
    SHS-0110-3 AccuTarget™ Human CK Kinase siRNA Subset 11 33
    SHS-0110-4 AccuTarget™ Human CMGC Kinase siRNA Subset 56 168
    SHS-0110-5 AccuTarget™ Human Other Kinase siRNA Subset 342 1026
    SHS-0110-6 AccuTarget™ Human STE Kinase siRNA Subset 43 129
    SHS-0110-7 AccuTarget™ Human TK Kinase siRNA Subset 84 252
    SHS-0110-8 AccuTarget™ Human TKL Kinase siRNA Subset 40 120
    SHS-0190-1 AccuTarget™ Human Other Phosphatase siRNA Subset 147 441
    SHS-0190-2 AccuTarget™ Human Tyrosine Phosphatase siRNA Subset 41 123



    siRNA Druggable Set

    Cat. No. Product Description No. of Genes No. of siRNAs
    SHD-00XX AccuTarget™ Human Druggable siRNA library set 8175 24,525
    Tuesday, 04 June 2013 10:31

    ProtoGel (30%) - 1 Litter

    37.5:1 Acrylamide to Bisacrylamide Stabilized Solution

    Optimized for SDS-PAGE of Proteins (Laemmli gels)
    Consistently Crystal Clear Gels, Zero Fluorescence
    Stabilized for Long Shelf Life

    Catalog Number: EC-890
    ProtoGel-30percent-1LitterProtoGel forms an electrophoresis matrix that is ideal for the separation of proteins and polypeptides. ProtoGel is a stabilized, ready-to-use 30% (w/v) acrylamide/methylene bisacrylamide solution (37.5:1 ratio), manufactured from the highest quality materials, from which virtually all impurities have been removed. ProtoGel has zero acrylic acid content, eliminating the fixed charges that cause band streaking. Additionally, oxidation products such as aldehydes have been removed by a selective adsorption process. Aldehydes can attack proteins, altering the structure of the sample, and thus altering Rf values. With ProtoGel, you can trust that your results will be consistent one electrophoretic run to the next.

    Storage: ProtoGel is stable for 24 months when stored tightly capped in a dark area at room temperature (20°C).

    ProtoGel (30%) Protocol
    Measure and Mix Solutions:
    The volume of ProtoGel required for gel casting solutions of any volume and acrylamide concentration may be calculated from the following formula:
    Vp =(X) (Vt)/30
    where:
    Vp = Volume of 30% ProtoGel
    X = % Monomer Desired in Gel
    Vt = Total Volume of Gel Casting Solution

    EXAMPLE: To make 100 ml of a 10% monomer gel, calculate the volume of Protogel to add as follows:
    Vp = (10) (100)/30 = 33.3 ml

    Initiate and Cast Gel:
    For optimal results degas gel solution for 10 minutes under vacuum aspiration prior to innitiation with APS and TEMED. Add 1.0ml of 10% (w/v) ammonium persulfate for every 100ml of gel casting solution. Swirl gently to mix. Add 0.1 ml of TEMED for every 100ml of gel casting solution. Swirl gently to mix. Pour the solution into the gel casting cassette. The gel should begin to set in 10-20 minutes. To provide a sharp interface, overlay the gel with water saturated n-butanol during polymerization. Flush butanol away with water just before casting the stacking gel (below).

    Cast Stacking Gel:
    Use ProtoGel Stacking Buffer to make 10ml of a 4% stacking gel:
    ProtoGel: 1.3ml
    ProtoGel Stacking Buffer: 2.5ml
    Deionized Water: 6.1ml
    Add 0.05ml 10% Ammonium Persulfate and 0.01ml of TEMED. Gel will begin to set in 20 minutes.

    PDF-IconDownload datasheet

    Price: 109 Euro

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    Thursday, 30 May 2013 09:58

    AccuLadder 100 bp DNA Size Marker

    More Sharp! High Resolution! More Convenience!

    AccuLadder 100 bp DNA size marker was designed to determine the size of double stranded DNA fragments from 100 to 2,000 bp. AccuLadder is shaper and brighter than our standard 100bp ladder The AccuLadder 100 bp DNA size marker consists of 13 double stranded molecular weight markers ranging in sizes from 100 to 1,000 bp in 100 bp increments, and additional fragments of 1,200, 1,600, 2,000 bp. The 500, 1,000 and 2,000 bp bands are two times brighter for easy identification.

    Note:
    The DNA ladder can be applied directly onto an agarose gel.
    There is no need to heat before loading. Repeated freezing and thawing should be avoided.

    Features and Benefits

    Complete and ready to load: Convenient
    Easy-to-Identify reference bands: Easy-to-read results
    Competitive pricing: Great value for your research dollar

     

    Specifications

    Concentration: 54 ng/μl
    Recommended loading: 4.0~5.0 μl / 5 mm lane width
    Typical Number of lanes: 126~157 (5 mm lane width)
    Size range (bp): 100 - 2,000
    Number of bands: 13
    Supplied in: 10 mM Tris-HCl (pH 8.0), 1 mM EDTA, 2.5% Ficoll, 0.005% Bromphenol Blue, 0.005% Xylene Cyanol
    Storage: -20°C

    acculadder100bpDNA figure1

    2.0% TBE agarose gel stained with Ethidium Bromide.

    Nuclease Activity Test

    acculadder100bpDNA figure2

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