Adapting Cells To a Serum-Free Environment
Fully defined, animal-component free and GMP produced cell culture supplement.
Performing cell culture without serum can be challenging. However, the rewards do largely recompense the efforts, and re-discove- ring the basics of cell culture develops quickly into a passion. The intention of this paper is to guide the user to a smooth transition to serum-free conditions and to avoid all inadequate or inappropriate efforts.
Ideally, the transition to serum-free conditions should be carried out over several passages to gradually select cells that can grow under serum-free conditions. However, direct adaptation to serum-free environments may also work out successfully, provided that all crucial aspects are addressed properly.
Regardless of the method used, key concerns include the growth state of the cellular inoculum, cell seeding density, sub-cultivation techniques, and biophysical attributes of the cell culture system.
Our XerumFree™ serum replacement has been designed so as to be used in the same way as conventional cell culture sera, as a medium supplement. The concentration however is 5x higher, so typically you will use 2% to a basal medium. You will go through the same steps as usual.
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