Pluri-EZ™ hESC/iPSC Culture Medium

Cat #: ASM-5014
Product Size: 100 ml
Background: The ability to culture human embryonic stem cells (hESCs) and Induced Plurpotent Stem Cells (iPSCs) under chemically defined conditions is a prerequisite not only for the production of hESCs/iPSCs under GMP conditions but, also the development of protocols that will be used for future preclinical/clinical studies (1). Attempts at the formulation of chemically defined tissue culture conditions have included: 1) The replacement of serum in the medium with a chemically defined substitute (2,3) and the replacement of a MEF feeder layer with a defined feeder free culture surface (4-6).

Figure 1: (a) iPSC passage 5 growth curves comparing Pluri-EZ™ to mTeSR™. No statistical difference was found in iPSC growth. Typical iPSC colony grown using Pluri-EZ™(b) and mTeSR™(c).
Product description: Pluri-EZ is chemical based media and highly stable.
Storage conditions: -20˚C; 1 year, 4˚C; 2 months. Minimize exposure to direct light.
Shipping: On dry ice

Notes:
1. Thaw the Pluri-EZ™ medium either overnight at 4˚C or for 20 minutes at RT.
2. Addition of bFGF and Activin A may help cell culture results.

Neuro-Sure™ Neural Crest Stem Cell Culture Media

Cat #: ASM-4021
Product Size: 100 ml
Background: One of the defining characteristics of vertebrate development is the migration of neural crest cells from the neural tube. Neural crest cells give rise to a variety of tissues such as melanocytes, some heart vessels, cephalic neuroendocrine organs, connective tissue of the craniofacial skeleton and the neurons and glia of both the sensory and autonomic ganglia of the peripheral nervous systems 1,2. The study of neural stem cells and their progenitors, Neural Crest Stem Cells (NCSCs), have direct medical implications. Numerous pathologies, such as skeletal and nervous system disorders and peripheral neuropathies, arise from aberrant migration, specification or differentiation of neural crest cells3,4. In order to assist in your neural crest cell studies, our NCSC media has been optimized to contain all the factors required to support NCSCs plated on fibronectin coated tissue culture surfaces.
Product description and usage: Add 2 mL of NCSC medium supplement to 498 mL of
NCSC culture medium to produce 500 mL of NCSC media.

Storage conditions for NCSC medium: -80˚C; 1 year, 4˚C; two weeks. Minimize exposure
to direct light.

Storage condition for NCSC supplement: -80˚C; 1 year, 4˚C; two weeks.

Storage condition for NCSC media: 4˚C; two weeks. Minimize exposure to direct light.

Shipping: On dry ice.

Recommended procedure:
1. Thaw NCSC medium either overnight at 4˚C or for several hours at RT.
2. Thaw the NCSC medium supplement at RT.
3. Using sterile technique, add the NCSC medium supplement to the NSCS medium. Mix thoroughly and store the newly made media at 4˚C for up to two weeks. NOTE: NCSC medium can be filter sterilized if desired. Do NOT filter sterilize the NCSC medium supplement. Add this supplement directly to the NCSC medium.
4. Prior to use, warm the media to RT in the tissue culture hood. Due to the complex composition of the NCSC media, we do not recommend warming the media in a 370C water bath.

ESC-Sure™ Serum-/Feeder- Free hESC/iPSC Culture Medium (SFFM)

Cat #: ASM-5010
Product Size: 100 ml
Our Serum, feeder-free medium formulations are optimized for human ESC and iPSC culture. It contains growth factors and extracellular matrix proteins secreted by MEF cells.

SFFM is ready-to-use.
- Only need to add FGF2
- Culture dish coating: matrigel or similar matrix.

Blastocyst-Sure™ KSOM Embryo Culture Medium, with Phenol Red (with Amino Acid)

Cat #: ASM-5023
Product Size: 1 Pack (8 ml X 3 vials)
Size: 1 Pack (8 mL X 3 vials)

Blastocyst-Sure™ embryo culture medium is designed for in vitro culture of mouse embryos. Our optimized formula ensures that mouse embryos develop to blastocyst stage as close as they do in vivo. Each batch of Blastocyst-Sure™ embryo culture medium passes a stringent embryo culture test before they are released.

Formulation: Frozen liquid
Recommended protocol:
1. After thawing, the Blastocyst-Sure™ embryo culture medium should be used within 2 weeks. If you need to store media for later use, store it at 4C in dark for up to 2 weeks. Avoid repeated freeze/thaw cycles.
2. Prepare drops of Blastocyst-Sure™ embryo culture medium in advance. Cover medium drops with light mineral oil (embryo tested grade) and equilibrate in a 37C, 5% CO2 incubator for a couple of hours before use.
3. Change to fresh medium every 24h during embryo culture.

Blastocyst-Sure™ KSOM Embryo Culture Medium, without Phenol Red (with Amino Acid)

Cat #: ASM-5024
Product Size: 1 Pack (8 ml X 3 vials)
Size: 1 Pack (8 mL X 3 vials)

Blastocyst-Sure™ embryo culture medium is designed for in vitro culture of mouse embryos. Our optimized formula ensures that mouse embryos develop to blastocyst stage as close as they do in vivo. Each batch of Blastocyst-Sure™ embryo culture medium passes a stringent embryo culture test before they are released.

Formulation: Frozen liquid
Recommended protocol:
1. After thawing, the Blastocyst-Sure™ embryo culture medium should be used within 2 weeks. If you need to store media for later use, store it at 4C in dark for up to 2 weeks. Avoid repeated freeze/thaw cycles.
2. Prepare drops of Blastocyst-Sure™ embryo culture medium in advance. Cover medium drops with light mineral oil (embryo tested grade) and equilibrate in a 37C, 5% CO2 incubator for a couple of hours before use.
3. Change to fresh medium every 24h during embryo culture.