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Monday, 11 March 2013 15:49

DNA Cloning Service

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With decades of experience our Molecular Biology Team has developed an exceptional expertise in anything DNA-related. We  can help you develop the best strategy to tackle your cloning projects and find solutions to all your technical problems. 

Some examples of our services:

  • - Cloning of amplified DNA fragments
  • - Gene targeting vectors for homologous recombination
  • - Bacterial Artificial Chromosome (BAC) Recombineering
  • - RNAi and inducible vectors
  • - Site-specific mutagenesis
  • - Tagging of genes
  • - De novo gene synthesis

 

A typical cloning procedure includes:

  • - Primer design for target region
  • - PCR amplification of target region
  • - Cloning of the PCR product into a cloning vector
  • - Sub-cloning the gene into the vector of your choice
  • - Picking, plating, culturing, and DNA preparation
  • - Sequence confirmation
  • - Plasmid purification (Mini, Midi, or Maxi size)
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