With decades of experience our Molecular Biology Team has developed an exceptional expertise in anything DNA-related. We can help you develop the best strategy to tackle your cloning projects and find solutions to all your technical problems.
Some examples of our services:
- - Cloning of amplified DNA fragments
- - Gene targeting vectors for homologous recombination
- - Bacterial Artificial Chromosome (BAC) Recombineering
- - RNAi and inducible vectors
- - Site-specific mutagenesis
- - Tagging of genes
- - De novo gene synthesis
A typical cloning procedure includes:
- - Primer design for target region
- - PCR amplification of target region
- - Cloning of the PCR product into a cloning vector
- - Sub-cloning the gene into the vector of your choice
- - Picking, plating, culturing, and DNA preparation
- - Sequence confirmation
- - Plasmid purification (Mini, Midi, or Maxi size)