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Monday, 11 March 2013 15:39

Teratoma Formation Analysis Services

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Service to confirm pluripotency of human and mouse ESC and iPSC lines by Teratoma Formation Analysisafter injection of cells into an animal.

Features:

  • - Turnaround time: 4 weeks (Mouse), 7 weeks (Human)
  • - Number of cells required / injection site: Mouse ESCs/iPSCs = 0.5 x 10^6, Human ESCs/iPSCs = 1 x 10^6
  • - Enhanced experimental protocol: Injection under both the kidney capsule and in testis results in higher efficiency of distinct teratoma formations with differentiated tissues derived from all three germ layers.

 

Service Includes:

  • - Comprehensive report with histological analysis and high resolution publication-grade images of endoderm, mesoderm and ectoderm formation.
  • - Tissue blocks and H&E stained tissue section slides

 

Notes:

  • - Frozen sample culture 
  • - Pathogen tests (please provide mycoplasma test result showing negative; otherwise additional testing fee will be required.) 
  • - Kidney capsule tumor and testis tumor from each mouse will be embedded in one block. Separate blocks are available upon request.
  • - Frozen tissue blocks are available.

 

Technical Details:

  • Experimental setup: A total of 4 mice (3 experimental and 1 control) will be used.
  • Injection site:Each recipient mouse will be injected at two locations: in the kidney capsule and in the testis.
  • Service includes:Cell injection, teratoma harvesting, tissue preparation (embedding and sectioning), H&E staining and histology analysis of teratoma tissue sections.

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Service to confirm pluripotency of human and mouse ESC and iPSC lines by Teratoma Formation Analysisafter injection of cells into an animal.

Features:

  • Turnaround time: 4 weeks (Mouse), 7 weeks (Human)
  • Number of cells required / injection site: Mouse ESCs/iPSCs = 0.5 x 10^6, Human ESCs/iPSCs = 1 x 10^6
  • Enhanced experimental protocol: Injection under both the kidney capsule and in testis results in higher efficiency of distinct teratoma formations with differentiated tissues derived from all three germ layers.

Service Includes:

  • Comprehensive report with histological analysis and high resolution publication-grade images of endoderm, mesoderm and ectoderm formation.
  • Tissue blocks and H&E stained tissue section slides

Notes:

  • Frozen sample culture Fee $500 per line
  • Pathogen tests (please provide mycoplasma test result showing negative; otherwise additional testing fee will be required.) $200 per line
  • Kidney capsule tumor and testis tumor from each mouse will be embedded in one block. Separate blocks are available upon request with additional $100 per line.
  • Frozen tissue blocks are available with additional $100.
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