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    Monday, 11 March 2013 15:16

    Neural Crest Stem Cell Culture medium + Supplement

    Rate this item
    (1 Vote)

    neuro1

    neuro2
    a) NCSCs forming characteristic “lacunae” during
    proliferation on ASC’s NCSC media (10X)
    b) NCSCs at a cell density ready for passaging.(20X)

    Background: One of the defining characteristics of vertebrate development is the migration of neural crest cells from the neural tube. Neural crest cells give rise to a variety of tissues such as melanocytes, some heart vessels, cephalic neuroendocrine organs, connective tissue of the craniofacial skeleton and the neurons and glia of both the sensory and autonomic ganglia of the peripheral nervous systems. The study of neural stem cells and their progenitors, Neural Crest Stem Cells (NCSCs), have direct medical implications. Numerous pathologies, such as skeletal and nervous system disorders and peripheral neuropathies, arise from aberrant migration, specification or differentiation of neural crest cells. In order to assist in your neural crest cell studies, our NCSC media has been optimized to contain all the factors required to support NCSCs plated on fibronectin coated tissue culture surfaces.
    Product description: Add 2 mL of NCSC medium supplement to 498 mL of NCSC medium to produce 500 mL of NCSC media.
    Storage conditions for NCSC medium: -80°C; 1 year, 4°C; two weeks. Minimize exposure to direct light.
    Storage condition for NCSC supplement: -80°C; 1 year, 4°C; two weeks.
    Storage condition for NCSC media: -80°C; 1 year, 4°C; two weeks. Minimize exposure to direct light.
    Shipping: On dry ice.
    Recommended procedure:
    1. Thaw NCSC medium either overnight at 4°C or for several hours at RT.
    2. Thaw the NCSC medium supplement at RT.
    3. Using sterile technique, add the NCSC medium supplement to the NSCS medium. Mix thoroughly and store the newly made media at 4°C for up to two weeks. NOTE: NCSC medium can be filter sterilized if desired. Do NOT filter sterilize the NCSC medium supplement. Add this supplement directly to the NCSC medium.
    4. Prior to use, warm the media to RT in the tissue culture hood. Due to the complex composition of the NCSC media, we do not recommend warming the media in a 37 C water bath.

     Price: 80 EUR

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