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    Monday, 11 March 2013 15:13

    Random Transgenic Mouse

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    We provide a service to generate transgenic mice by traditional pronuclear microinjection. Using this method, transgenes are inserted randomly into the genome.

    Expression of the transgene and the subsequent transgenic mouse phenotype may vary from line to line due to the nature of random gene insertion. We recommend to generate multiple independent trangenic mouse lines in order to interpret the experimental results.

    Available mouse strains: FVB, C57BL/6

    We also offer Knock-in and Knock-out Mice and Knock-in and Knock-out Rat services. For more information please contact us.

    Generation of transgenic mice by random insertion

    Step 1: Cloning of cDNA, promoter of interest, and other elements

    Step 2: Construction of the transgene vector

    Step 3: Pronuclear microinjection of vector DNA into mouse embryos

    Step 4: Transfer of injected embryo into recipient mothers

    Step 5: Genotyping of offspring to identify potential transgenic founders

    Step 6: Breeding to F1 or F2 progenies (optional)

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