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Monday, 11 March 2013 14:50

TARGATT Knock-in Mice (3-months)

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Targatt can create site-specific knock-in mice for you within 3 months. Using our novel TARGATT system, a gene of interest can be inserted at a well-characterized, transcriptionally-active locus in the mouse genome with guaranteed transgene expression. Tissue-specific and/or ubiquitous expression options are available. Please contact us for a list of plasmid construct with reporter genes. 

In addition to our full service we also offer individual service options to generate your TARGATT knock-in mouse.

You can prepare the DNA using the TARGATT Kit and we do the pronuclear microinjection for you. Or we can prepare the DNA that you then inject after setting up your own TARGATT mouse colony.

Please contact us to discuss your project plan for your fast & site-specific knock-in or TARGATT Knock-down Mouse services.

* Prices are a general guideline for academic institutes and may vary.  Please inquire for quote.

Advantages of TARGATT Technology:

Site-specific DNA integration (knock-in) in transcriptionally-active locus

  • - Ensures robust gene expression
  • - Eliminates unpredictable phenotypes caused by random integration
  • - Allows for proper comparison of different transgenes

Single-copy integration

  • - Eliminates repeat-induced gene silencing and genomic instability.

 

Insertion of intact transgene

  • - Avoids truncated transgenes with unexpected phenotype

High integration efficiency

  • Save time and costs!

 

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Applied Stem Cell Inc.’s proprietary TARGATT Technology enables highly efficient site-specific gene integration in mammalian cells and animals. This technology uses PhiC31 integrase to insert any gene of interest into a specific docking site that was pre-engineered into an intergenic and transcriptionally active genomic locus. Our TARGATT Technology improves several aspects in the generation of transgenic cell lines and animals:

1. High integration efficiency mediated by  PhiC31 integrase reduces time and cost

2. Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high expression levels of the transgene

3. Integration at intergenic region ensures that no internal genes are interrupted

4. Single copy gene integration eliminates repeat-induced gene silencing and genomic instability

5. Site-specific integration allows a precise comparison of the effects of the transgenes among different lines.

TARGATT Technology can be utilized for a variety of applications including reporter gene expression, gene knockdown and disease models.

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