GENTAUR Europe BVBA
Genprice Inc, Logistics
Serbia, 
Macedonia, 
Montenegro, 
Croatia: 
GENTAUR Romania
GENTAUR Greece 
AccuPower RocketScript RT PreMix contains Gentaur’s exclusive M-MLV based thermostable reverse transcriptase (RTase), RocketScript. Native M-MLV RTase has maximum activity at relatively low temperatures (42°C), causing several problems in reverse transcription of RNA molecules with complex secondary structure.
RocketScript has thermostable activity (42°C~70°C), allowing efficient cDNA synthesis from virtually any RNA. The lyophilized PreMix contains all components necessary for a successful reverse transcription reaction, including RTase, RNase inhibitor and buffer components. Just add template RNA, primers and water, and the RT reaction is ready to go.
Schematic representation of the 5’UTR of a gene, with complex secondary structure, at three different temperatures. Note that RocketScript shows full activity at 70°C allowing it to synthesize the complete gene sequence where M-MLV and other Reverse Transcriptase's fail.
Features and Benefits
Thermostable Activity: RocketScript is able to perform reverse transcription reactions throughout a wide range
of temperatures from 42°C to 70°C.
Enhanced Performance: RocketScript has enhanced performance to handle both high and low input RNA
concentrations as well as short and long RT target sizes.
Easy-to-use: The product contains the enzyme itself, plus RNase inhibitors and all other components necessary for the best reverse transcription results in the tube.
Reproducibility: Gentaur's strict quality controlled production system ensures that your results will be reproducible experiment after experiment.
Convenient: Just add template and primers and start your reaction. dNTPs, buffer and enzyme are provided.
Stability: Stable at room temperature for a month one year at 4°C and for 2 years in a -20°C freezer
RNase, DNase and Proteinase-free: Ensures the integrity of your samples.
Broad range of working temperatures: For RNAs that are G:C rich or significant secondary structure
Stable for 2 years at -20°C: Long shelf life
Specifications
5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: No
Fragment Size: Up to 10 kb
Application
- First-strand synthesis of cDNA from RNA molecules (RT)
- RT-PCR
- Random priming reaction
- Library construction
- Probe labeling
- mRNA 5end Mapping by Primer Extension Analysis
- Real time PCR
Figure 1. Sensitivity comparison between AccuPower RocketScript RT PreMix and M-MLV RTase
Sensitivity results of AccuPower RocketScript RT PreMix using GAPDH compared with conventional Reverse transcriptases.
Each 100 ng – 10 fg of total RNA used for RT and the same amount of RT products used for electrophoresis.
Lane 1: 10 fg Human total RNA from HeLa cell Lane 2: 100 fg Human total RNA from HeLa cell
Lane 3: 1 pg Human total RNA from HeLa cell Lane 4: 10 pg Human total RNA from HeLa cell
Lane 5: 100 pg Human total RNA from HeLa cell Lane 6: 1 ng Human total RNA from HeLa cell
Lane 7: 10 ng Human total RNA from HeLa cell Lane 8: 100 ng Human total RNA from HeLa cell
Figure 2. Comparison of amplification efficiency between AccuPower RocketScript RT PreMix and competitorsM-MLV RTase.
RocketScript is able to handle a wide range of sample concentrations and transcript lengths so your downstream applications are minimally effected by the reverse transcription step.
Lanes 1-3: 1,000 ng, 100 ng and 10 ng of total RNA from HeLa cells, respectively.
Figure 3. Sensitivity comparison between AccuPower RocketScript RT PreMix and competitor RTases using Real Time PCR
Reverse transcription conditions: conventional 1 hr incubation at 60°C, deactivation at 95°C for 5 min All cDNAs were amplified with AccuPower DualStar™ qPCR PreMix (K-6110) from Gentaur.
| Concentration | RocketScript RT PreMix | Supplier Q | Supplier I |
| 10,000 | 23.91 | 25.63 | 24.43 |
| 1,000 | 27.33 | 28.92 | 28.03 |
| 100 | 30.62 | 32.42 | 30.88 |
| 10 | 33.63 | 35.43 | 33.95 |
| Efficiency | 104% | 103% | 108% |
| Linearity | 0.99999 | 0.9996 | 0.9995 |
