The AccuPower RT PreMix is a master mix for cDNA synthesis that consists of an easy to resuspend, lyophilized mix ofM-MLV Reverse Transcriptase, RNase inhibitor, dNTPs, reaction buffer, tracking dye, and patented stabilizer. The master mix kit is used for first strand cDNA synthesis from RNA. Downstream applications include cDNA amplification with reverse transcription PCR. All of the key components are premixed at optimal concentrations. Simply add template RNA, primers and water to start your reaction.
For cDNA amplification with Cyclic RT, please see our AccuPower CycleScript RT PreMix.
Features and Benefits
| Convenient lyophilized RT: | Easy to use, simply add your purified RNA |
| High yield of cDNA: | For genes up to 9 kb within 10 minutes |
| Stable for 2 years at -20°C: | Long life |
| RNase, DNase and Proteinase-free: | Ensures the integrity of your samples |
Specifications
5' to 3' exonuclease: No
3' to 5' exonuclease: No
3’ – A Overhang: No
Fragment size: 9 kb
Application
cDNA synthesis
Figure 1. Reliability and reproducibility test with AccuPower RT PreMix. Each template showcases amplified target genes.
Lane M: 100 bp DNA Ladder (D-1030)
Lane 1-4: Reliability test of each lot with AccuPower RT PreMix
