Description:

JETSTAR is a new and unique anion exchange resin
developed by Gentaur. This new resin is supplied in disposable
columns (Mini, Midi and Maxi columns) , which are used
under gravity flow conditions without further instrumentation.
The JETSTAR Plasmid Kits were designed by combining
JETSTAR Columns with a modified alkaline/SDS lysis proce-
dure for the preparation of plasmid DNA. Gentaur´s product
is the ideal tool to achieve ultrapure plasmid DNA within 45

Procedure :The procedure employs a modified alkaline/SDS method to prepare the cleared lysate. After neutral-ization, the lysate is applied onto a JETSTAR Column (Mini, Midi or Maxi) and the plasmid DNA is bound to the anion exchange resin . Washing the resin removes RNA and all other impurities. Finally the purified plasmid DNA is eluted from the column and concentrated by an alcohol precipitation. 

Plasmid yields :Yields of up to 20 µg (Mini), 100 µg (Midi) and 500 µg (Maxi) of plasmid DNA can be expected using the JETSTAR columns. All types and sizes of plasmid DNA can be prepared, but yields depend very much on the plasmid copy numbers (low/medium/high), the type  f plasmid, the bacterial strain and the volume of bacterial culture used. The recovery of plasmid DNA is on average between 85% and 90%.

Plasmid purity :The JETSTAR-purified plasmid DNA is of higher quality than 2 x CsCl purified plasmid DNA. Corre- spondingly, its recommended application range is extremely wide, including transfection, microinjection, vaccination, gene therapy, fluorescent and radioactive sequencing, amplification, in vitro transcription, cloning, etc.

Culture V olumes and DNA Yields
JETSTAR Kits are preferentially designed to extract and purify high copy plasmid DNA from E.coli cultures. Low copy plasmids can be prepared as well, but larger culture volumes have to be used.
Gentaur recommends LB medium to grow E.coli cells to prepare plasmid DNA with JETSTAR columns. The JETSTAR system is however compatible also with other growth media, especially those designed for enhanced plasmid yields . The cell density should be approximately 1 x 10 cells per ml medium (1-1.5 A 600 units/ml).

High Copy Plasmids (3 - 5 µg DNA/ml LB medium)

A.	Culture Volume	DNA Yield
Mini	1 -5 ml	3 - 20 µg
Midi	15 - 25 ml	45 - 100 µg
Maxi	100 ml	300 - 500 µg

 

Low Copy Plasmids   (0.2 - 1 µg DNA/ml LB medium)

A.	Culture Volume	DNA Yield
Mini	10 - 15 ml	2 - 45 µg
Midi	25 - 100 ml	5 - 100 µg
Maxi	250 - 500 ml	50 - 500 µg

 

Product pricelist:

Product	Contents	Cat.#	Price:
JETSTAR 2.0 Plasmid Midiprep Kit / 25	25 Midi Columns Solutions, Reagents	2210025	126 €
JETSTAR 2.0 Plasmid Midiprep Kit / 50	200 Midi Columns Solutions, Reagents	210050	202 €
JETSTAR 2.0 Plasmid Midiprep Kit / 250	250 Midi Columns Solutions, Reagents	210250	721 €
JETSTAR 2.0 Plasmid Midiprep LFU Kit / 25	50 Midi Columns Solutions, Reagents	211025	186 €
JETSTAR 2.0 Plasmid Midiprep LFU Kit / 100	10 Midi Columns Solutions, Reagents	211100	547 €
JETSTAR 2.0 Plasmid Maxiprep Kit / 10	10 Maxi Columns Solutions, Reagents	220010	126 €
JETSTAR 2.0 Plasmid Maxiprep Kit / 20	20 Maxi Columns Solutions, Reagents	220020	202 €
JETSTAR 2.0 Plasmid Maxiprep Kit / 100	100 Maxi Columns Solutions, Reagents	220100	769 €
JETSTAR 2.0 Plasmid Maxiprep LFU Kit / 20	20 Maxi Columns Solutions, Reagents	221020	289 €