Product description
This kit is the best option for customers wanting to generate cDNA ready for real-time applications. The Kit provides a rapid and very sensitive method for first strand cDNA synthesis for use in real -time PCR studies. The TransAmp™ Buffer provides highly optimized components for efficient reverse transcription and includes a unique blend of anchored oligo dT and random hexamer primers to ensure unbiased 3’ and 5’ coverage for enhanced data accuracy. An extremely efficient reverse transcriptase delivers highly robust first strand synthesis and higher cDNA yields from a wide range of input RNA concentrations. SensiFAST cDNA Synthesis Kit offers enhanced reproducibility, sensitivity and efficiency for exceptional performance in subsequent real-time PCR experiments.
Feature: | Benefit: | Advantege: |
Reproducible | Unique reverse transcriptase and new TransAmp™ buffer generate consistent first-strand cDNA |
Reduces the number of replicates the customer needs to do, saving space and cost |
Broad dynamic range |
Ideal for dilute and low-copy samples | Improves assay sensitivity as abundant and rare transcripts can be run against each other |
Sensitive | Real-Time RT-PCR analysis from as little as 4pg of starting total RNA |
Can be used even when starting material is limited |
Unbiased cDNA synthesis |
Complete 5' to 3' RNA sequence representation |
Gives more accurate quantification of genes |
Rapid | cDNA synthesis and real-time PCR in less than 60 minutes |
Allows higher throughput |
Optimized | Designed to complement the SensiFAST SYBR and Probe kits |
Can be used directly in a real-time assay without further optimization needed |
Positioning
The SensiFAST cDNA Synthesis Kit has been optimized for real-time reactions, the new TransAmp Buffer contains reverse transcriptase enhancers that reduce complex RNA secondary structure, so that efficient reverse
transcription of all RNA samples, regardless of the complexity, can be achieved at 42° C, resulting in unparalleled reproducibility between samples.
Cat. | Product | Price | Order |
BIO-65053 | SensiFAST cDNA Synthesis Kit, 50 Reactions | 262 € | |
BIO-65054 | SensiFAST cDNA Synthesis Kit, 250 Reactions | 980 € |
More: cDNA kits