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Background staining or non-specific staining is an often-encountered problem in immunohistochemistry, in immunofluorescence and in situ stains. Background staining is caused by a number of factors such as cross reactivity of antibodies with the shared epitopes in the tissue, by the presence of natural and/or contaminating antibodies present in the primary antibody and/or the secondary antibody, by ionic interactions, by the presence of carbohydrates and by endogenous biotin present in the tissue. Eradicating background is most important for obtaining background-free specific staining for the ease of qualitative and quantitative evaluation.
PRODUCT DESCRIPTION
Background Buster is a peptide Blocker that eradicates all general background staining. Background Buster removes all background staining caused by primary antibodies, by the staining reagents, by the chromogens, by the fixatives and by endogeneouse biotin present in tissues such as liver and spleen and kidney. Background Buster is used in place of normal sera and other blocking solution for removing background staining in both human and animal tissues.
Background Buster is applicable IHC staining, to immunofluorescence staining and to in situ probe staining in both human and animal tissues. It is also applicable to flow cytometric assays.
Background Buster is a must for animal tissue staining, it is especially essential when staining identical species tissue and antibodies such as mouse antibodies on mouse tissues (Mouse-on-Mouse) and Rabbit-on- Rabbit. A 30-minute incubation with Background Buster is recommended prior to the application of the primary antibody for staining of identical species primary antibodies and tissues. The use of Background Buster is highly recommended for staining of indirect species (non-identical species tissue/ antibody) such as Rat –On-Mouse, Mouse-on- Rat, Mouse-on-Rabbit, etc. A 20-minute incubation with Innovex Background Buster is recommended prior to the application of the primary antibody for staining of Indirect (non-identical species primary antibodies and tissues).
In immunoperoxidase-IHC staining, another type of background and non-specific staining is caused by red blood cell staining; this is due to endogenous peroxidase enzyme present in red blood cells. This type of background requires a pre-treatment step with 3% freshly made hydrogen peroxide (H2O2) in water, this blocking step should precede the blocking step with Innovex Background Buster.
NB306 Background Buster 125 ml qty 419 EUR
NB306-50 Background Buster 50 ml qty 325 EUR
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Immunolocalization of electrogenic sodium-bicarbonate cotransporters pNBCI and kNBCI in the rat eye
IMMNLINOLOCALIZATION OF SODIUM.BICARBONATE COTRANSPORTERS
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