Contact Us

GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it. 

Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR France

     GENTAUR France SARL
    9, rue Lagrange, 75005 Paris 
    Tel 01 43 25 01 50 
    Fax 01 43 25 01 60
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    Gentaur Germany

    This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-size: 12px; line-height: 1.3em;">

      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
    Fax (+49) 0241 56 00 47 88 This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-family: Arial, Tahoma, Verdana, Helvetica; line-height: 15.59375px; ">
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-size: 12px; line-height: 1.3em;">

    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Nederland

     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
    This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Spain

     GENTAUR Spain
    Tel 0911876558
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    Genprice USA

    usa-flagGenprice Inc, Logistics
    547, Yurok Circle
    San Jose, CA 95123
    Phone/Fax: 

    (408) 780-0908 

    This email address is being protected from spambots. You need JavaScript enabled to view it.

    skype chat

    GENPRICE Inc. invoicing/ accounting:
    6017 Snell Ave, Suite 357
    San Jose, CA. 96123

     

    Gentaur Serbia

    serbiaSerbia, Macedonia FlagMacedonia, 

    montenegro-flagMontenegro, croatiaCroatia: 
    Tel 0035929830070 
    Fax 0035929830072
    This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Romania

    romGENTAUR Romania

    Tel 0035929830070 
    Fax 0035929830072
    This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Greece

    grGENTAUR Greece 

    Tel 00302111768494 
    Fax 0032 16 50 90 45

    This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
    Danmark +4569918806
    Österreich +43720880899
    Ceská republika Praha +420246019719
    Ireland Dublin +35316526556
    Norge Oslo +4721031366
    Finland Helsset +358942419041
    Sverige Stockholm +46852503438
    Magyarország Budapest +3619980547

    seal-in-search-symantec

     

     

    Tuesday, 03 September 2013 17:23

    New method for turning genes on and off could enable more complex synthetic biology circuits

    Rate this item
    (1 Vote)

    CRISPR-mice-virus-detection-teratoma-teratoma-service-ipsc-transgenic-mouseMIT researchers have shown that genes on or off in yeast and human cells by controlling, when DNA into RNA - bypokrok that will allow scientists to better understand the function of these genes copy.
    This technique can also be easier to control the environment of the cell produkujídrogy or disease realize engineer, says Timothy Lu, assistant professor of electrical engineering and computer science and bio - engineering and lead author of a paper describing the new approach in the journal ACS Synthetic Biology.
    "I think it will be much easier to build synthetic circuits" says Lu, a member of the Synthetic Biology Center at MIT. "It should increase the extent and speed with which we can build a number of synthetic circuits in yeast and mammalian cells."
    The new method is based on a system of viral proteins have been used recently for the treatment of genomes of bacteria and human cells. Initial system called CRISPR consists of two parts: a protein which binds to DNA and washers and a short strand of RNA, leaders of the protein into the correct position in the genome.
    "CRISPR system is so powerful that it can be used for a variety of DNA - binding regions of a simple conversion of this handbook RNA targeted basis" says Lu. "By simply reprogramming the RNA sequence of this protein can be any place you want to call the genome or synthetic circuit."
    The main author of the article is Farzadfard Fahim, an MIT graduate student of biology. Samuel Perl, student of Electrical Engineering and Computer Science, is takéautorem.

    Targeting transcription

    In previous studies CRISPR was used to cut pieces of the gene is switched off or replace it with another gene. Lu and his colleagues decided to use the CRISPR system for a different purpose : control of gene transcription is the process by which DNA sequence into RNA (mRNA ), which goes beyond genes copied instructions.
    Transcription is strictly regulated by proteins called transcription factors. These proteins bind to specific DNA sequences in the promoter region of the gene, and either modify or block the enzymes needed for the copy of the gene into mRNA.
    Which acts as a transcription factor for this study, the researchers adjust CRISPR system. Firstly, the modified CRISPR normal protein known as time9 so that it no longer cut the DNA bound thereto. They are also added to the protein, the segment that is activated or suppressed by modulating gene expression machinery of cellular transcription.
    Time9 to get to the right place, the researchers also corresponds delivered to the target cells, the genes for RNA guides who chtějísekvence DNA activates the promoter of the gene.
    Researchers have shown that when the guide RNA and protein time9 connection in the target cell, the target gene přesněpravý atranskripce. To their surprise, the same complex time9 also used to block transcription to be found in other parts of the gene.
    "It's nice that allows you to make a positive and negative regulation of the same protein, but with different RNA targeted to various managerial positions in the promoter" says Lu.

    "A lot of elasticity"

    The new system should be much simpler than other newly developed two systems for the transcription of DNA - binding proteins such as zinc finger transcriptional activator and effector Nucleases ( Talens ) is known, says Lu. Although effective, the construction and assembly of proteins is time- consuming and expensive.
    "There is a lot of flexibility with CRISPR, and it really comes from the fact that you do not spend more time in protein engineering. You can only change the sequence of the nucleic acid RNA, " says Lu.
    "The fact that this can be used for effective regulation of transcription in both yeast and mammalian cells, it is very encouraging," says Kobi Benenson, Professor of Biosystems Science and Engineering at ETH Zurich, which is not part of the research team." This technology may be used in the very near future genetic engineering and synthetic biology applications for biopharmaceuticals - "Tissue engineering and gene therapy, among other things"
    Researchers also transcriptional control designed so that it can be triggered by specific small molecules, which may be included in the cell, such as sugars. For this, the guide RNA genes constructed so that they are only produced Primal molecule is available. No small molecules, there are no guidelines aRNA gene is targeted at rest.
    This type of control could be used to explore the role of naturally occurring genes on and off at specific times during development or progression useful, says Lu.
    Lu is now working on the development of advanced synthetic circuits for applications such decisions are based on multiple inputs made ​​by the mobile environment. " We want to be able to scale- up the most complex circuits and show that anyone is ever built in yeast and mammalian cells" he says.

    Read 3326 times