Contact Us

GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it. 

Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR France

     GENTAUR France SARL
    9, rue Lagrange, 75005 Paris 
    Tel 01 43 25 01 50 
    Fax 01 43 25 01 60
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    Gentaur Germany

    This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-size: 12px; line-height: 1.3em;">

      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
    Fax (+49) 0241 56 00 47 88 This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-family: Arial, Tahoma, Verdana, Helvetica; line-height: 15.59375px; ">
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-size: 12px; line-height: 1.3em;">

    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Nederland

     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
    This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Spain

     GENTAUR Spain
    Tel 0911876558
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    Genprice USA

    usa-flagGenprice Inc, Logistics
    547, Yurok Circle
    San Jose, CA 95123
    Phone/Fax: 

    (408) 780-0908 

    This email address is being protected from spambots. You need JavaScript enabled to view it.

    skype chat

    GENPRICE Inc. invoicing/ accounting:
    6017 Snell Ave, Suite 357
    San Jose, CA. 96123

     

    Gentaur Serbia

    serbiaSerbia, Macedonia FlagMacedonia, 

    montenegro-flagMontenegro, croatiaCroatia: 
    Tel 0035929830070 
    Fax 0035929830072
    This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Romania

    romGENTAUR Romania

    Tel 0035929830070 
    Fax 0035929830072
    This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Greece

    grGENTAUR Greece 

    Tel 00302111768494 
    Fax 0032 16 50 90 45

    This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
    Danmark +4569918806
    Österreich +43720880899
    Ceská republika Praha +420246019719
    Ireland Dublin +35316526556
    Norge Oslo +4721031366
    Finland Helsset +358942419041
    Sverige Stockholm +46852503438
    Magyarország Budapest +3619980547

    seal-in-search-symantec

     

     

    Wednesday, 14 August 2013 12:20

    DiaEasy Dialyzer Tubes

    Rate this item
    (1 Vote)

    A.   Problem

    While working with proteins and nucleic acids, it is very often necessary to eliminate small molecular weight substances such as reducing agents [dithiothreitol (DTT), 2-mercaptoethanol (BME), urea], un-reacted crosslinking or labeling reagents (sulfo-SMCC, biotin) and preservatives (sodium azide, thimerosal) that might interfere in subsequent steps of the experimental protocol. Methods such as dialysis and electro-elution (for extraction of nucleic acids from gels) have long been used to purify proteins and nucleic acids. However, these traditional methods are riddled with numerous problems which may lead to tremendous time consumption, sample loss, contamination and high sample dilutions requiring further concentration.

    dialyzer dialysis tubes gentaur antibodies

    B.   Solution

    DiaEasy™ Dialyzer tubes product line offers a solution to most of these issues. Our Dialysis tubes come in easy to use sets of 10, 25 and 100 tubes each, catering to different molecular weight cut-offs of 1 kDa, 3.5 kDa, 6-8 kDa, 12-14 kDa, 25 kDa and 50 kDa for different sample volumes ranging from 10 µl to 20 ml.  These sets also contain floating racks and/or supporting trays for convenient handling of the tubes in the exchange buffer and electroelution. The combination of DiaEasy™ Dialyzer tubes and the electroelution accessories provides a unique tool for extraction of any protein, protein-protein, or protein DNA complexes from non-denaturing and denaturing (SDS) polyacrylamide gels, and for extraction of oligonucleotides, RNA, and DNA from both polyacrylamide and agarose gels. The DiaEasy™ tube’s membranes are ultra-clean, sulfur and heavy metal free and EDTA treated which makes the harvested proteins and nucleic acids suitable for molecular biology experiments. These DiaEasy™ tubes allow rapid, secure and simple loading and recovery. They are very high performance dialysis tubes and are the most convenient, user friendly dialysis system on the market. We offer these tubes with related accessories like supporting trays and floating racks for easy use.

    C.   Applications of DiaEasy™ Dialyzer tubes

    • •      Dialysis or buffer exchange of six different samples volumes: 10-250 µl, 50-800 µl, 0.1-3 ml, 10 ml, 15 ml and 20 ml.
    • •      High throughput dialysis (using a single beaker with several DiaEasy™ tubes on one floating pad).
    • •      Preparation of protein samples for MALDI-MS.
    • •      Samples concentration.
    • •      Large-scale protein dialysis such as antibodies and recombinant protein purification.
    • •      Peptide dialysis as small as 10 amino acids.
    • •      Virus-particle purification.
    • •      Removal of contaminating micro molecules.
    • •      Tissue culture extracts purification.
    • •      Removal of salts, surfactants, solvents & detergents.
    • •      Complex formation studies (protein-protein, protein-DNA and protein-RNA).
    • •      pH and buffer adjustment of sample solution, protein extract or cell extract.
    • •      2D gel dots extraction.
    • •      Extraction of proteins, RNA, DNA or oligonucleotides (>20 nt) from polyacrylamide, agarose or any gel matrix in any running buffer.  

     

    D.   Advantages of DiaEasy™ Dialyzer tubes

    • •       Most convenient, efficient and user-friendly dialysis sets on the market.
    • •       Single step efficient dialysis saves time and eliminates the need for additional equipment.
    • •       Samples pass through the tubes once, eliminating repeated dilutions and concentrations.
    • •       Single step decreases the risk of potential protein aggregation and precipitation and ensures high activity retention.
    • •       Elution times are as low as 10 minutes and range upto 150 minutes based on multiple criteria including the sample content, size and volume.
    • •       These sets are HTP compatible.

     

    E.   Typical Recovery Rates

    Dialysis of samples

    ≥ 98%

    Electroelution of DNA or RNA from Agarose gel

    ≥ 90%

    Electroelution of DNA or RNA from polyacrylamide gel

    ≥ 90%

    Electroelution of Protein from SDS-PAGE gel

    ≥ 70%

     

    F.   Advantages of DiaEasy™ Dialyzer tubes over conventional dialysis methods:

     

    Conventional Dialysis

    DiaEasy™ Dialyzer Tubes

    Advantages

    Multistep protocol

    Single step

    Time efficiency

    High potential for protein aggregation and precipitation

    Minimal potential for protein aggregation and precipitation

    Eliminates need for post dialysis sample treatment before use in downstream applications

    Potential for loss in protein activity

    Retains maximum protein activity due to minimal processing time

    Ensures highly usable protein for any downstream applications

    Non-high throughput compatible

    High throughput compatible

    Can be used for large-scale protein dialysis such as antibodies and recombinant protein purification.

    Low sample recovery/yield

    ≥ 98% protein recovery/yield

    High yield

    High contamination

    Low potential for contamination

    Purer sample recovery

    Requires additional equipment

    No requirement for any other equipment

    Simple to use

    Requires membrane preparation

    Ready to use

    No preparation required

    Can lead in sample leakage

    No sample leakage

    Maximum recovery

     

    Order Button1

    Read 1620 times