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Friday, 21 June 2013 15:33

AccuTargetâ„¢ Custom Designed siRNA Synthesis

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Gene silencing by RNA interference (RNAi) technology using small interfering RNA (siRNA) is a powerful tool for studying functional genomics, drug target identification and validation, pathway elucidation, and therapeutic development. The advantage is in the algorithm we use for siRNA design. Our Turbo si-Designer software identifies highly effective siRNA target sites with remarkably high siRNA knockdown rates. Critical parameters including base composition, thermodynamic instability and base preference are all considered in the design algorithm. siRNAs spanning SNP sites are removed and finally, non-specific siRNAs are eliminated following homology searching by BLAST and Smith-Waterman algorithms. The result is an siRNA with extraordinary siRNA knockdown efficiency and minimal off-target effects. The performance of Turbo si-Designer has been extensively evaluated by testing the siRNA knockdown efficiency of thousands of siRNAs targeting STE Kinases, TK Kinases, genes involved in the NF-kappaB and Caspase Pathways using Real-time PCR analysis. The results of the evaluation indicated the design algorithm was highly effective in selecting effective siRNAs; 80% of the tested siRNAs showed > 70% knockdown and 38% elicited knockdown of > 90%.

 

Cat. No. Guaranteed Yield Purification
S-1017-5 1 nmole BioRP
S-1017-6 5 nmole
S-1017-1 10 nmole
S-1017-2 20 nmole
S-1017-3 50 nmole
S-1017-4 100 nmole
S-1018-5 1 nmole HPLC
S-1018-6 5 nmole
S-1018-1 10 nmole
S-1018-2 20 nmole
S-1018-3 50 nmole
S-1018-4 100 nmole

 

- siRNA provided lyophilized and annealed.
- Annealing buffer is provided when single-stranded siRNA requested.

 

 

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