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    Thursday, 13 June 2013 17:00

    EnzyChrom Adipolysis Assay Kit

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    EnzyChrom Adipolysis Assay Kit

    Quick Overview:
    Quantitative assay of adipolysis by measuring glycerol released in cell culture using colorimetric (570nm) or fluorimetric (530nm/590nm) methods. Procedure: 20 min. Detection limit: 0.92 g/mL. Shelf life: 3 months. Shipping: on ice; storage: 4, -20C.

    Description:

    Obesity is a chronic condition that develops from storage of excessive energy in the form of adipose tissue. The resulting adiposity presents a high risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. ADIPOLYSIS or lipolysis is a  ighly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery. BioAssay Systems'  dipolysis assay kit directly measures glycerol released during adipolysis. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product  t 570nm is directly proportional to glycerol concentration in the sample.

    Applications:

    - Direct Assays: adipolysis (glycerol in cell culture media).
    - Drug Discovery/Pharmacology: effects of testing drugs on adipolysis.

    Cat. No.: EAPL-200

    Quantity / Size: 200 Tests

    Kit Contents:

    Assay Buffer: 24 mL Enzyme Mix: 500 μL ATP: 250 μL Dye Reagent: 220 μL Standard: 100 μL 100 mM Glycerol Storage conditions. The kit is shipped on ice. Store Assay Buffer at 4°C and other reagents at -20°C. Shelf life of 12 months after receipt. Precautions:  eagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.

    Benefits:

    - Assay kit that are simple, convenient and cost effective

    - Superior in performance

    - Researchers need little-to-notime for assay optimization

    - Specialize for both routine laboratory tests and for high-throughput drug discovery applications

    - with a focus on safe, non-radioactive assay

    Key Features: Key features of our assays include simplicity, high-throughput, sensitivity, accuracy and low interference.

    Storage Temp: -20°C

    Shipping Conditions: Dry Ice

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