FEATURES
USE - Measure PGE2 between 1,000 - 31.25 pg/mL
SAMPLE - Lysates, Saliva, Urine, Serum, Plasma and Tissue
SAMPLES/KIT - 40 or 232 in Duplicate
SENSITIVE - Measure < 3 pg per Sample
VERSATILE - Works directly with Mouse and Rat Serum
DESCRIPTION
The DetectX® Prostaglandin E2 (PGE2) Immunoassay kits are designed to quantitatively measure PGE2 present in serum, plasma, urine, saliva and tissue culture media samples. Standards or diluted samples are pipetted into an antibody coated microtiter plate. A PGE2-peroxidase conjugate is added to the wells and the binding reaction is initiated by the addition of a monoclonal antibody to PGE2. After a 2 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound PGE2-peroxidase conjugate. After a 30 minute incubation, the reaction is stopped and the plate read at 450nm.
Prostaglandins are synthesized from arachidonic acid by cyclooxygenase (COX)-1 or -2, which convert the acid into PGH2. This is further processed by cytosolic or microsomal prostaglandin synthases to become PGE2 or one of several other prostanoids. Prostacyclin is the major cyclooxygenase product in blood vessel walls and it is present in inflammatory fluids in similar concentrations to PGE2. PGE2 is a potent vasodilator and produces hyperalgesia. PGE2 is produced by a wide variety of tissues and in several pathological conditions, including inflammation, arthritis, fever, tissue injury, endometriosis, and a variety of cancers.
Item | Catalog # | Order | Price |
---|---|---|---|
PGE2 Enzyme Immunoassay Kit, 1 Plate | K018-H1 | Click Here or Here | 301 € |
PGE2 Enzyme Immunoassay Kit, 5 Plate | K018-H5 | Click Here or Here | 1016 € |