Contact Us

GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it. 

Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
Tel 0035924682280 
Fax 0035929830072
This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR France

     GENTAUR France SARL
    9, rue Lagrange, 75005 Paris 
    Tel 01 43 25 01 50 
    Fax 01 43 25 01 60
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    Gentaur Germany

    This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-size: 12px; line-height: 1.3em;">

      GmbH Marienbongard 20
    52062 Aachen Deutschland
    Tel (+49) 0241 56 00 99 68 
    Fax (+49) 0241 56 00 47 88 This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-family: Arial, Tahoma, Verdana, Helvetica; line-height: 15.59375px; ">
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    This email address is being protected from spambots. You need JavaScript enabled to view it." style="font-size: 12px; line-height: 1.3em;">

    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    This email address is being protected from spambots. You need JavaScript enabled to view it.

    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
    Tel 020 3393 8531 
    Fax 020 8445 9411
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Poland

     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
    Tel  058 710 33 44
    Fax 058 710 33 48 
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Nederland

     GENTAUR Nederland BV
    Kuiper 1 
    5521 DG Eersel Nederland
    Tel 0208-080893 
    Fax 0497-517897
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    Gentaur Italy

     GENTAUR SRL IVA IT03841300167

    Piazza Giacomo Matteotti, 6, 24122 Bergamo
    Tel 02 36 00 65 93 
    Fax 02 36 00 65 94
    This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Spain

     GENTAUR Spain
    Tel 0911876558
    This email address is being protected from spambots. You need JavaScript enabled to view it." style="">This email address is being protected from spambots. You need JavaScript enabled to view it.

    Genprice USA

    usa-flagGenprice Inc, Logistics
    547, Yurok Circle
    San Jose, CA 95123
    Phone/Fax: 

    (408) 780-0908 

    This email address is being protected from spambots. You need JavaScript enabled to view it.

    skype chat

    GENPRICE Inc. invoicing/ accounting:
    6017 Snell Ave, Suite 357
    San Jose, CA. 96123

     

    Gentaur Serbia

    serbiaSerbia, Macedonia FlagMacedonia, 

    montenegro-flagMontenegro, croatiaCroatia: 
    Tel 0035929830070 
    Fax 0035929830072
    This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Romania

    romGENTAUR Romania

    Tel 0035929830070 
    Fax 0035929830072
    This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

    GENTAUR Greece

    grGENTAUR Greece 

    Tel 00302111768494 
    Fax 0032 16 50 90 45

    This email address is being protected from spambots. You need JavaScript enabled to view it.">This email address is being protected from spambots. You need JavaScript enabled to view it.

    Other countries

    Other countries
    Luxembourg +35220880274
    Schweiz Züri +41435006251
    Danmark +4569918806
    Österreich +43720880899
    Ceská republika Praha +420246019719
    Ireland Dublin +35316526556
    Norge Oslo +4721031366
    Finland Helsset +358942419041
    Sverige Stockholm +46852503438
    Magyarország Budapest +3619980547

    seal-in-search-symantec

     

     

    Saturday, 18 May 2013 17:20

    InfectoSTOP 25ml

    Rate this item
    (1 Vote)

    Initiating a primary cell culture from a surgical tissue is often difficult because of contamination. It is therefore important to incubate the tissue in an appropriate solution containing an optimized mixture of antibiotics, each at a given concentration able to avoid infectious contamination without affecting cell viability. Furthermore, it is not always possible to initiate primary cell culture immediately after surgical excision so the tissue needs to be stored for several hours until further processed.

    Product Description

    InfectoSTOP (GENT 19 - INSTOP) is a ready-to-use solution containing an optimized mix of antibiotics against gram-negative and gram-positive bacteria, mycoplasma and fungi for cell culture applications.

    Simply dilute the necessary volume (enough to cover the tissue) of InfectoSTOP in PBS or in cell culture medium.

    Intended Use

    InfectoSTOP is intended for use in primary cell culture initiation or to extend cell viability in fresh surgical material of any tissue type.

    InfectoSTOP can also be used for decontaminating established cell cultures.

    The product should be handled under sterile conditions and is not intended for animal or human use.

    Caution: If handled improperly, some components of this product may present a health hazard. Take appropriate precautions when handling this product, including the wearing of protective clothing and eyewear. Dispose of properly

    How to use InfectoSTOP

    To initiate primary cultures from fresh surgical material

    Dilute InfectoSTOP 10x in PBS (4 °C) and put the tissue in the freshly made solution. Rinse tissue immediately twice or more and then incubate 2 hours at 4 °C. Prepare a fresh solution, rinse once and incubate overnight (or at least one week without viability loss, depending on the type of tissue) at 4 °C.

    Just before initiating primary cell culture, rinse one more with InfectoSTOP.

    To eliminate contamination in anchorage-dependant cells
    • - Entirely rinse the flask twice with a 5x diluted fresh made InfectoSTOP solution (w/o serum)
    • - Incubate at 37 °C for 1 hour
    • - Entirely rinse the flask twice with a 10x diluted freshly made InfectoSTOP solution (w/o serum)
    • - Incubate at 37 °C for 3 hours
    • - Entirely rinse the flask twice with a 10x diluted freshly made InfectoSTOP solution (w/o serum)
    • - Incubate overnight in cell culture with a 10x diluted freshly made dilution of InfectoSTOP in complete culture medium

    The above cell treatment can be repeated twice if necessary.

    The dilution and incubation time of InfectoSTOP must be adjust to your own cell culture

    To eliminate contamination in anchorage-independent cells

    Same procedure as above, but before rinsing and incubation, pellet the cells by brief centrifugation.

    Storage and Stability
    •  InfectoSTOP is stored under -20° C at our facility and is shipped on dry ice.
    •  If the product is to be used immediately, thaw in a 37° C water bath or overnight at 4° C.
    •  If thawed in a water bath, do not leave the product at 37° C for more than 1 hour.
    •  When stored at 4° C, InfectoSTOP is stable for at least 2 weeks.
    •  If the product is not to be used within 1 week after receipt, we recommend storing it at below -20° C in a freezer that is not self-defrosting.
    •  Do not thaw and refreeze more than once. When stored at below -20°C, the product is stable until the expiration date shown on the label.

    Handling:

    GLP techniques should be employed for the safe handling of this product.

    This includes observing the following practices:

    • - Wear appropriate laboratory cloches including a lab coat, gloves and safety glasses.
    • - Do not mouth pipette, inhale, ingest or allow to come into contact with open wounds. Wash thoroughly any area of the body, which comes into contact with the product.
    • - Avoid accidental autoinoculation by exercising extreme care when handling in conjunction with any injection device.
    • - Handle the product under sterile area.
    • - This product is intended for research purposes and should be handled by qualified personnel only. It is not intended for use in humans or in animals. Gentaur is not liable for any damages resulting from the misuse or handling of this product.
    Components

    One bottle of 25 ml.

    Order Button1

    Read 1239 times