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GENTAUR Ltd.
Howard Frank Turnberry House
1404-1410 High Road
Whetstone London N20 9BH
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ul. Grunwaldzka 88/A m.2
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Piazza Giacomo Matteotti, 6, 24122 Bergamo
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San Jose, CA 95123
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6017 Snell Ave, Suite 357
San Jose, CA. 96123
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Articles
Nanoliter injector
For oocyte injection and applications in the 2 to 70 nanoliter range
WPI’s microprocessor-controlled Nanoliter 2000 uses direct piston displacement. By either pushing the injection button on the control box or pressing on the optional footswitch, a calibrated volume will be smoothly injected. The process is quiet and vibration free. Capillary filling and injection speeds are 23 nL/sec and 46 nL/sec (emptying speeds are 92 nL/sec and 230 nL/ sec). Maximum fluid ejection is 5 μL. Each unit comes with sufficient glass to pull at least 300 tips. Glass is 1.14 mm O.D. (nominal) and 0.5-mm I.D. By setting the DIP switch, the injection volume can be changed from 2.3 to 69.0 nL in 16 steps. Up to 100 injections may be triggered per filling. Since the volume of a normal Xenopus oocyte is about 500 nL, the instrument has the capability to inject from less than 1% to over 10% of the total volume of the oocyte in one preset step increment. Included: 1 vial 3.5 in. capillaries (100); 1 vial 7 in. Capillaries (100); replacement “O” rings; Allen wrench; MicroFilTM MF34G backfilling needle; and two sample μTip™ pre-pulled micropipettes.
Smart Controller: Micro4, an optional microprocessor-based controller, can provide an “intelligent” and easy-to-use interface to up to four Nanoliter Injectors. Operating parameters are set with the membrane keypad and LCD display. From the keypad the user can set pump to infusion or withdrawal mode, enter the volume to be infused or withdrawn, and rate of delivery, as well as synchronize the starting and stopping of a combination of Injectors. User parameters can be stored in the device’s “non-volatile” memory for instant recall when the unit is powered on. An optional footswitch can be plugged into a connector on the rear of the controller for “hands free” start/stop operation. An RS-232 port on the rear of the controller can be used to connect it to a computer for use with computer control programs.
NANOLITER 2000
SPECIFICATIONS
INJECTION VOLUME Variable
REMOTE CONTROL Yes
GLASS OD 1.14 mm
GLASS ID 0.5 mm
STEP 12.7 μ/step
INJECTION SPEED
Slow 23 nL/sec
Fast 46 nL/sec
FILL SPEED
Slow 23 nL/sec
Fast 46 nL/sec
EMPTY SPEED 92 nL/sec
VARIABLE VOLUME RANGE 2.3 - 69.0 nL
SMALLEST VOLUME 2.3 nL
TO CHANGE VOLUME Set switch
INJECTIONS PER FILLING, MAX. 100 injections
SHIPPING WEIGHT 3 lb. (1.1 kg)
