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GENTAUR Europe

 GENTAUR Europe BVBA
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45 
Fax 0032 16 50 90 45
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Gentaur Bulgaria

 GENTAUR BULGARIA
53 Iskar Str. 1191 Kokalyane, Sofia
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    GENTAUR France

     GENTAUR France SARL
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    Gentaur London

     GENTAUR Ltd. 
    Howard Frank Turnberry House 
    1404-1410 High Road 
    Whetstone London N20 9BH 
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     GENTAUR Poland Sp. z o.o. 

    ul. Grunwaldzka 88/A m.2

    81-771 Sopot, Poland
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     GENTAUR Nederland BV
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    Piazza Giacomo Matteotti, 6, 24122 Bergamo
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     GENTAUR Spain
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    547, Yurok Circle
    San Jose, CA 95123
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    Other countries
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    Articles

    HoloMonitor M3

    holomonitor-gentaur-antibodies-pcr-elisaAt present , the commonly used cell microscopy observations require staining of the cells or mark , or by external excitation source for cell imaging analysis , but these markers and long exposure often have some damage to cells and even cause cell death , can not be informed cell longevity real situation . Another traditional end-point detection , and can only get a simple end result , unable to understand the dynamics of cellular processes , or even lose some important information , which lead to inaccurate or erroneous conclusions. Thus , a need for a very non-invasive real-time non-destructive imaging analysis cell culture solution to the above problem. Currently on the market of non -invasive techniques , such as: electrical impedance and phase imaging technology, although no damage to the cell , but there are still some drawbacks. Phase contrast and interference field microscopy imaging techniques can shoot high-contrast images , electrical impedance sensing technology can detect the relative cell number and morphology changes , but not for cell imaging . They do not reflect the real three-dimensional cell morphology, including cell parameters such as the thickness and volume , can be quantitatively analyzed.
     
    HoloMonitor ™ M3 using a digital holographic microscopy (DHM), a laser source of coherent light , the use of the object wavefront recorded interference principle of phase and amplitude information , a single holographic samples , specimens of different depth to digital reconstruction images and reproduces true three-dimensional specimen image , while access to a wealth of quantitative information, which contains a non-invasive real-time conditions, the optical thickness of living cells and other cellular morphological parameters and dynamic information . A hologram requires only a single exposure can obtain three-dimensional structure of the specimen quantitative information , so do not need to scan the sample can have a laser scanning confocal microscope for three-dimensional imaging advantages. Another M3 equipped with powerful analysis software system , which includes a top of the image processing module , can not only provide the entire cell culture system where information such as quantity, less focused , sports migration , etc., can also provide each image in a single cell morphological parameters such as area , thickness, volume , roundness , and other irregularities . Therefore , M3 is both a high -definition 3D microscope , it is a super cell analyzer .
     
    HoloMonitor ™ M3 features:
    ① no marks , non-invasive , non-destructive monitoring system for the cells without damage , restore the most authentic recording cell growth state .
    ② quantitative holographic imaging technologies, access to the most comprehensive dynamic cells , including conventional microscopes can not get away from the morphological parameters such as cell thickness and volume .
    ③ do not harm cells do not kill cells , therefore , prolonged cell culture, for the same sample of cells can be detected continuously .
    ④ cell culture apparatus no special requirements applicable to any standard dishes , flasks , perforated plates, slides , etc.
    ⑤ two kinds of automatic and manual focus mode , the focusing depth of up to 4mm, and the samples are always in focus without blurring the image.
    ⑥ imaging to distinguish cells in the background threshold manually after automatic calibration and image correction function can be restored after many recalculations and imaging, to further improve image clarity.
    ⑦ image capture can be a single cell or a group of a region , there can be multiple regions groups also can time-lapse imaging . The resulting both in a conventional 2D image rendering , it can be reconstructed 3D image .
    ⑧ cell auto-recognition feature , split cells artificial color, automated cell counting , both isolated single cells analyzed separately , it can be arbitrarily divided into regional groups for comparative analysis .
    ⑨ morphological measurements and data output , and the resulting data can be in XML format and other image formats JPEG, TIFF, bitmap, PNG, GIF , etc. output.

     

    HoloMonitor M4

     

    holomonitor-m4-gentaur-antibodies-pcr-elisa

    Microscopy technique Holographic transmission microscopy
    Sample stage            Fixed
    Light source External monochromatic laser
    Camera 1280×1024 pixel CMOS
    Objective 20×
    Software HoloStudio M4/Time-laspe or optionally HoloStudio M4/Tracker

     

     

    System performance

    Lateral resolution 1 µm
    Field of view ~0.5 mm2
    Working distance 1.2 mm
    Digital focusing range ~0.25 mm
    Exposure time 5 ms, non-scanning
    Maximum image acquisition rate 1 image/s
    Sample illumination ~0.1 mW/cm2
    Maximum height of cell culture chamber 45 mm

    Computer requirements

    Windows 7

    Power requirements

     
    Input voltage 100-249 VAC 47-63 Hz
    Power consumption  
    Microscope unit Maximum 6 W

    Laser unit

    Maximum 15 W

    Physical characteristics

     
    Dimensions (L×W×H) 250×160×180 mm
    Weight 2.5 kg

    Sample requirements

     
    Cells Mono-layer of adherent eukaryotic cells in any of the supported cell culture vessels. Eukaryotic suspension cells require than an IBDI µ-slide is used as cell culture vessel.
    Cell culture vessels

    T75, T25, 6-well plate and IBIDI µ-slide