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    Articles

    HALO-96 PRT

    A Predictive Residual Toxicity Assay for Primitive Lympho-Hematopoietic Stem Cells

    HALO®-96 PRT Applications

    - To determine the proportion of primitive lympho-hematopoietic stem cells remaining or resistant to drug administration.
    - To determine whether primitive lympho-hematopoietic stem cells change their sensitivity to a drug after a single dose.
    - To determine the effect on primitive stem cells of multiple drug administration.
    - To determine whether primitive lympho-hematopoietic stem cells exhibit expansion potential after a single drug dose.

    Advantages of using HALO-96 PRT to Detect Residual Stem Cell Toxicity

    - Cheaper and more efficient than animal testing (3Rs) and can be applied directly to fresh, primary human cells.
    - The only in vitro assay available that can detect whether primitive stem cells are available for potentially repopulating the lympho-hematopoietic system after a single drug dose.
    - The only assay available that indicates changes in drug sensitivity to primitive stem cells.
    - Uses a 2-stage primitive stem cell "priming" and cell expansion in vitro re-plating technology.
    - Incorporates Suspension Expansion Culture (SEC) and proven Bioluminomics™ Technology.
    - Designed for multiplexing with other assays, including flow cytometry and gene expression analysis, to obtain the most information from a single sample.

    Species

    - Human
    - Non-human primate
    - Dog
    - Rat
    - Mouse
    - as well as horse, pig and sheep.

    Tissues

    HALO®-96 PRT is only for use with bone marrow from the above species.

    Cell Populations Tested

    HALO®-96 PRT is only used for the primitive lympho-hematopoietic stem cell populations:

    - HPP-SP 1
    - HPP-SP 2

    Use

    For Research Use Only.

    Major Equipment Required

    Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

    Kit Contents

    - HALO®-96 PRT Master Mix
    - ATP standard
    - ATP high and low controls
    - ATP Enumeration Reagent
    - 2 or 4 sterile, 96-well plates
    - Non-sterile, 96-well plates
    - Sterile, adhesive foil covers
    - Instruction manual