Articles

HALO-96 Research

Proliferation Assays for Human Lympho-Hematopoietic Stem and Progenitor Cell Research

HALO®-96 Research Applications

- For all human and animal lympho-hematopoietic research applications.
- Detect and measure proliferation of human and animal stem and progenitor cells.
- Measure and compare proliferation status and/or potential between tissues and cell populations.
- Experimental transplantation models.
- Effects of growth factors and/or cytokines.
- In vivo to in vitro assays.
- Gene targeting assays.

Advantages of using HALO®-96 Research

- HALO®-96 Research is the only assay system available to detect and measure lympho-hematopoietic proliferation of human and animal cells. (The colony-forming cell, CFC, does not measure proliferation; it is a differentiation assay because colonies are identified by the differentiation and maturation of the cells that form the colonies. Please see CAMEO™-4 and CAMEO™-96). See HALO® and CFC Information Flyer.
- Distinguish between multiple primitive and mature stem cell populations and lineage-specific populations.
- Reliably compare your results from one experiment to another and over time by calibrating and standardizing the assay (reagents provided).
- Provides all the flexibility you need for your research, including adding your own growth factors and/or cytokines.
- HALO® utilizes Suspension Expansion Culture (SEC) and bioluminomics™ Technology that can be easily multiplexed with other assay readouts to obtain the most information from the same sample.

Species

HALO®-96 Research is available for the following species:

- Human
- Non-human primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus))
- Horse
- Pig
- Sheep
- Dog
- Rat
- Mouse

Individual Human and Animal Cell Populations Detected using HALO®-96 Research (see Lympho-Hematopoietic System to view cell populations and growth factor/cytokine cocktails)

The following proliferation status or potential can be detected for individual populations using HALO®-96 SPCA:

Stem Cells

• HPP-SP 1: IL-3, IL-6, SCF, TPO, Flt3-L

• HPP-SP 2: EPO, GM-CSF, IL-2, IL-3, Il-6, IL-7, SCF, TPO, Flt3-L

• CFC-GEMM 1: EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L

• CFC-GEMM 2: EPO, GM-CSF, IL-3, IL-6, SCF, TPO

• CFC-GEM 1: EPO, GM-CSF, IL-3, IL-6, SCF

• CFC-GEM 2: EPO, GM-CSF, IL-3, SCF

• CFC-GEM 3: EPO, GM-CSF, G-CSF, IL-3, SCF

Progenitor Cells

• BFU-E 1: EPO, IL-3, SCF

• BFU-E 2: EPO

• GM-CFC 1: GM-CSF, IL-3, SCF

• GM-CFC 2: GM-CSF, G-CSF, IL-3, SCF

• GM-CFC 3: GM-CSF

• Mk-CFC 1: TPO, IL-3, SCF

• Mk-CFC 2: TPO

Lymphopoietic cells

• T-CFC: IL-2 (requires addition of co-stimulator)

• B-CFC: IL-7

No growth factors: Add growth factors/cytokines of choice.

Use

For Research Use Only. Not for clinical diagnostic use

Major Equipment Required

Plate luminometer or multiparameter plate reader with "glow" luminescence measuring capability.

Kit Contents

- HALO®-96 Research Master Mix
- ATP standard
- ATP high and low controls (not included with research kits)
- ATP Enumeration Reagent
- Sterile, 96-well plates
- Non-sterile, 96-well plates
- Sterile, adhesive foil covers
- Instruction manual