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General Kits

BioReady Taq pac.

BioReady Taq is a highly pure, thermostable recombinant DNA polymerase encoded by a modified gene from Thermus aquaticus species and expressed in E.coli. Its recombinant nature ensures utmost purity, reproducibility and processivity. BioReady Taq processes an initial 5’→3’ exonuclease activity and lacks 3’ →5’exonuclease activity. The enzyme leaves a single 3’-nucleotide overhang that makes the product for cloning by TA vector system. BioReady Taq provides a strong thermostability that meets the requirements of specialized PCR applications.
This product includes all components except primers. Users only need to prepare primers and template to run PCR. The operation is rapid and simple.
Features
Good thermostable
High activity
The PCR product with this enzyme is available to run TA clone directly
The product is suitable for real-time PCR reaction owing to 5’-3’ exonuclease activity.

Application
PCR raction
TA clone
Sequence

Experimental Data
1. Length of PCR product and PCR efficiency 2. BioReady Taq in Real-Time PCR

BioReady Hot Start Taq pac

The BioReady Hot Start Taq is a developed method to minimize the side effects during PCR. The BioReady Hot Start Taq inhibits Taq polymerase activity during RCP reaction preparation. By limiting Taq polymerase activity at low temperature prior to PCR cycling, non-specific amplification and primer-dimers are reduced.
Features
High-Specificity
High activity
The PCR product with this enzyme is available to run TA clone directly.
The product is suitable for realtime PCR reaction owing to 5’-3’ exonuclease activity.
Application
PCR reaction
TA clone
Sequence
Experimental Data

BioReady Pfu pac.

BioReady Pfu is a thermostable enzyme of approximately 92 KD isolated from pyrococcus furiosus. BioReady Pfu catalyzes the DNA-dependent polymerization of nucleotide into duplex DNA in the 5’-3’ direction in the presence of magnesium ions. The enzyme also exhibits 3’-5’ exonuclease (proofreading) activity. The base misinsertions that may occur infrequently during polymerization are rapidly emendated by the proofreading activity of the polymerase. In generally, BioReady Pfu is mainly used for polymerization reactions requiring high fidelity synthesis.
Features
High fidelity
Good thermostable
High activity
5’-3’ exonuclease activity
Application
PCR reaction
Sequence

Experimental Data
1. Electrophoresis result

2. Sequence result

RT 007 AMV Reverse Transcriptase

RT007 AMV reverse transcriptase was isolated from avian myeloblastosis virus (AMV) as the holoenzyme of molecular weight 157,000 daltons, using a modification of the method described by houts et al. this preparation is free of nuclease. It is qualified for cDNA synthesis and also for dideoxy sequencing of DNA and RNA. total RNA or poly a+ RNA can be used as template, and optimal temperature is 42℃~-55℃, up to 60 ℃. if nappi is used, 37-41℃ is preferred. RT reaction buffer covered by patents can be used either as 5x /10x stock depending on different purpose (details in standard application). up to 10-12kb cDNA may be obtained according to standard application.

Features:
high-sensitivity
high-yield
patented rt reaction buffer is suitable for many kinds of applications
up to 10-12kb cdna
up to 60 ℃ reaction temperature effectively opens the secondary structure of rna.

Experimental data

Red Blood Cell Lysis Buffer

Red blood cell lysis buffer was developed to lyse red blood cells from human whole blood. the buffer provides a very simple, fast economic way to isolate white blood cells from whole blood without destroying the white blood cells. In general, with the buffer, you can get 80% or more white blood cells from the sample.
Features
Lysis rapidly and adequately
The isolated wbc (white blood cells) are very pure.
The yielded wbc have good biology activity and are beneficial for downstream experiments.
The isolated wbc can be applied to nucleic acid extraction, pcr and so on.
The yielded products can be directly applied to the flow cytometer and the purity of cell is high.

Lymphocytes Separation Medium

Lymphocytes Separation Medium uses a density grads principle to isolate lymphocytes (including monocytes). The media is used to isolate lymphocytes and monocytes from human peripheral blood. In general, with the buffer, you can get 70% or more lymphocytes and monocytes from the sample.
Features:
The isolated lymphocytes have high purity;
The operation is convenient and time-saving;
Using high quality Meglumine Diatri-zoate to make Lymphocytes Separation Medium, the separated monocytes has a high purity and yield.
Procedure

Application
Lymphocytes Separation Media can be used to isolate lymphocytes and
monocytes from 1ml~ 5 ml human peripheral bloods. Yielded intact lymphocytes and
monocytes for further use.
This media is not intended to deal with the whole bloods in animal’s species.

Pheol

Due to the corrosive nature of the phenol, preparation of phenol solution for use in a molecular biology laboratory is a time-consuming and ofen hazardous procedure. Frequently it is necessary to redistill the phenol prior to use to remove contaminants and oxidation products that can damage nucleic acids. In some cases, the PH phenol solutions needs to be adjusted before use. Safety precautions such as protective eyewear, gloves, and a fume hood are necessary when you use the product.
Features
We have a full line of products such as equilibrated phenol, mixed phenol, saturated phenol and so on.
Manufactured under the standard of ISO9001 and strict quality control
It can be applied for RNA and DNA extraction and there is no contamination of RNase, DNase enzyme
We provide convenient, safe and multiple types of phenol solutions to customers to avoid many complicated and dangers operations.
Procedure
Efficient extraction of cell extracts or solutions containing nucleic acids are most often performed with a series of phenol and phenol-chloroform extractions at a specific pH. Both phenol and chloroform cause proteins to become denatured and become soluble in the organic phase or interphase, while nucleic acids remain in the aqueous phase. After centrifugation, the aqueous phase containing nucleic acids is re-extracted with an equal volume of chloroform-isoamly alcohol. This combination of extractions is to reduce the loss of RNA due to the formation of insoluble protein: RNA complexes at the interphase.
Chloroform is mixed with phenol to increase the efficiency of nucleic acid extractions by reducing losses of RNA at the interphase. The increased efficiency is due to chloroform’s ability to denature proteins and aid in the removal of lipids, thus improving separation of nucleic acid into the aqueous phase. Phase separation is also enhanced, which assists in the removal of the aqueous phase with minimal cross contamination from the organic phase. In general, isoamyl alcohol is added to phenol-chloroform to reduce foaming.