Articles

Spin Column Extraction Kits

Biospin Plasmid Extraction Kit

The kit provides a fast, simple, and cost-effective plasmid miniprep method for routine molecular biology laboratory applications. plasmid dna can be purified from 1~5ml of overnight cultures of e. coli. the dna isolated by this kit is ready for downstream applications such as restriction enzyme digestion, sequencing, pcr/real-time pcr and other downstream experiments.

 

Biospin PCR Purification

Biospin PCR purification kit provides a simple, rapid and effective method to purify dna fragments from pcr or enzymatic reaction. dna fragments ranged from 60bp to 10kb can be purified. the yield of dna with size lower than 100bp is 23~95%, while with size from 0.1kb to 10kb is 90~97%. purified dna can be used directly in downstream molecular biological experiments such as cloning, sequencing, restriction enzyme digestion, pcr/real-time pcr and so on.

Attribute:

Item	Description
specification	50t and 100t
sample volume	100μl
dna fragment range	60bp-10kb
dna purity	1.8~2.0 (a260/a280)
elute yield	≥99%
operation time	≤6 min

 

Biospin Gel Extraction Kit

Biospin gel extraction kit provides a simple, rapid and effective method to purify dna fragments from agarose gel in tae or tbe buffer. dna fragments ranged from 60bp to 23kb are purified from (up to 3% standard or high/low-melt) gel using spin column. purified dna can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, restriction enzyme digestion and so on.

Biospin Tissue Extraction Kit

Biospin tissue genomic DNA extraction kit provides a very simple, fast and economic way to isolate pure high–molecular-weight genomic dna from animal tissues. adopting the genomic dna buffer set, the simple purification procedure, based on the remarkable selectivity of biospin membrane, allows isolation of high yields of pure genomic dna in 40min. it doesn’t require any expensive equipment, or toxic reagents. in general, 10-30 μg genomic dna can be acquired from up to 50 mg tissue. the pure dna can be applied extensively in pcr/real time pcr, sequencing, southern blot, mutant analysis, snp and the others.
Procedure
kinds of tissues and cells

Biospin Whole Blood Extraction Kit

Biospin blood genomic dna extraction kit provide a very simple, fast and cost effective technique to isolate pure high–molecular-weight genomic dna direct from whole blood treated with commonly used anticoagulants such as citrate, heparin, edta, and also suitable for leukocytes, by the genomic dna buffer set,. the simple purification procedure, based on the remarkable selectivity of biospin membrane, allows isolation of high yields of pure genomic dna in 30min. it doesn’t require any expensive equipment, or toxic reagents. in general, 2-6μg genomic dna can be acquired from 200μl blood. the pure dna can be applied extensively in pcr/real-time pcr, sequencing, southern hybridization, mutant analysis, snp and the others.
Principle
DNA is liberated by pk solution and lb buffer. released dna is bounded exclusively and specifically to the biospin membrane at the presence of binding buffer under appropriate salt iron and ph conditions. denatured protein and other contaminants are removed with several washing procedures. the dna is then eluted from the membrane with elution buffer.

Experimental data
①Sample: 200μl whole blood
electrophoresis result: m: λ/ ecor ⅰ+ hind ⅲ

② The result of scanned absorbency

Biospin Cell Genomic DNA Extraction Kit

Biospin cell genomic dna extraction kit provides a very simple, fast and economic way to isolate pure high–molecular-weight genomic dna from all kinds of cultured cells. the simple purification procedure, based on the remarkable selectivity of biospin membrane, allows isolation of high yields of pure genomic dna in less than 1 hour. it doesn’t require any expensive equipment, or toxic reagents. the pure dna can be applied extensively in pcr, sequencing, southern blot, mutant analysis, snp and the others.
Experimental data
①sample:
1.5*105 cells, absorbance test: 1.8≤a260/a280≤2.0
② contrast results: b: biospin
q: one of the famous trademarks of usa
p: one of the famous trademarks 105 pieces of cells
③ contrast with q by real-time pcr: 50~106 cells (the blue curve is biospin, the red curve is q)

Biospin Plant Genomic DNA Extracction Kit

Biospin plant genomic dna extraction kit provides a very simple, fast and economic way to isolate pure high–molecular-weight genomic dna from plant tissues. the simple purification procedure, based on the remarkable selectivity of biospin membrane, allows isolation of high yields of pure genomic dna less than 40min.it doesn’t require any expensive equipment, or toxic reagents. generally, 1-30 μg genomic dna can be acquired from up to 100 mg plant tissue. the pure dna can be applied extensively in pcr/real-time pcr, sequencing, southern blot, mutant analysis, snp and the others.
Compared with brand q and brand t:
b: Biospin q: the product from q company t: the product from t company
1. Paddy leaf, marker: λ/ ecor ⅰ+ hind ⅲ

Biospin Fungus Genomic DNA Extraction Kit

Biospin fungus genomic dna extraction kit provides a very simple, fast and economic way to isolate pure high–molecular-weight genomic dna from fungus. the simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic dna less than 1 hour. it doesn’t require any expensive equipment, or toxic reagents. 2.5-30 μg genomic dna can be acquired from up to 100 mg tissue or up to 5ml yeast culture. the pure dna can be applied extensively in pcr/real-time PCR, sequencing, southern blot, mutant analysis, snp and the others.
Experimental data
sample: 2ml cultured yeast (30℃, 48h), 1~6#1~10# are same test, marker: λ/ ecor ⅰ+ hind ⅲ

Biospin Bacteria Genomic DNA Extraction Kit

Due to the corrosive nature of the phenol, preparation of phenol solution for use in a molecular biology laboratory is a time-consuming and ofen hazardous procedure. Frequently it is necessary to redistill the phenol prior to use to remove contaminants and oxidation products that can damage nucleic acids. In some cases, the PH phenol solutions needs to be adjusted before use. Safety precautions such as protective eyewear, gloves, and a fume hood are necessary when you use the product.
Features
We have a full line of products such as equilibrated phenol, mixed phenol, saturated phenol and so on.
Manufactured under the standard of ISO9001 and strict quality control
It can be applied for RNA and DNA extraction and there is no contamination of RNase, DNase enzyme
We provide convenient, safe and multiple types of phenol solutions to customers to avoid many complicated and dangers operations.
Procedure
Efficient extraction of cell extracts or solutions containing nucleic acids are most often performed with a series of phenol and phenol-chloroform extractions at a specific pH. Both phenol and chloroform cause proteins to become denatured and become soluble in the organic phase or interphase, while nucleic acids remain in the aqueous phase. After centrifugation, the aqueous phase containing nucleic acids is re-extracted with an equal volume of chloroform-isoamly alcohol. This combination of extractions is to reduce the loss of RNA due to the formation of insoluble protein: RNA complexes at the interphase.
Chloroform is mixed with phenol to increase the efficiency of nucleic acid extractions by reducing losses of RNA at the interphase. The increased efficiency is due to chloroform’s ability to denature proteins and aid in the removal of lipids, thus improving separation of nucleic acid into the aqueous phase. Phase separation is also enhanced, which assists in the removal of the aqueous phase with minimal cross contamination from the organic phase. In general, isoamyl alcohol is added to phenol-chloroform to reduce foaming.

Biospin Polyacylamide Gel DNA Extraction Kit

Biospin polyacrylamide gel dna extraction kit provides a simple, rapid and effective method to purify dna fragments from polyacrylamide gel. the simple purification procedure is based on the remarkable selectivity of biospin membrane and won’t affect the results when different staining methods are used. purified dna can be used directly for kinds of downstream molecular biological experiments such as cloning, sequencing, restriction enzyme digestion and so on.
Experimental data
result of different dna fragments
m: dl2000 marker s: before extraction r: after extraction

Comparison among different brands: b:biospin o: the product from o company t: the product from t company

SimplyP Total RNA Extraction kit

Simply p total rna extraction kit is a ready-to-use reagent to isolate total rna from various sources including whole blood, animal/plant tissue, cultured cell and bacteria. add solution r2 to the processed sample and transfer the mixture to spin column, and then total rna can be easily isolated through several washing and eluting steps. simply p total rna extraction kit provides a very simple, fast and economical technique to isolate high quality rna in high-throughput. the pure rna can be applied extensively in northern blot, blotting hybridization, poly(a)+ selection 、in vitro translation 、rnase protect assay, rt-pcr/real time rt-pcr analysis、construction cdna library etc.
Features:
Fast: ready-to-use high quality rna within 9 minutes
Simple: only 4 steps for extraction rna from samples
Easy: ready-to-use reagents. no need of professional during extraction operation.
no phemol/chloroform extraction, no cscl gradients, no licl or ethanol precipitation.
Suit for many kinds of samples
Excellent recovery of rna of starting samples from very small to large amount.
Mouse liver total rna extraction