Articles

LineGene I

LineGene I is a complete Real-time PCR detection system that can detect and quantitate nucleic acid sequences by monitoring fluorescence value during the PCR process. With innovative design on hardware and software, the unit is an ideal tool for research use in the field of gene analysis.

Features
•  Long life blue led light source
•  Extremely sensitive pmt detection module
•  Sandwich thermo-electronic (te) module
•  Easy yet powerful software
•  Data export

Multiple applications

Hardware

•  Thermal-electronic (te, peltier) module
The special designed sandwich te module fits both sides of the sample block well so that can deliver the temperature rapidly and reduce the vertical temperature gradient across different sample blocks, which ensures accurate controlling of pcr reaction temperature.

•  Blue led light source with a sensitive pmt detection module
The long life blue led light source doesn't need maintenance or preheating. And the sensitive photomultiplier tube (pmt) offers high sensitivity, broad dynamic range and low crosstalk. With the innovative design to put the light source and the detection module on the bottom of the sample tube, the measurement to the fluorescence is extremely sensitive. 

•  Use of hot-lid realizes oil free operation

High sensitivity and accuracy
By adopting coaxial optical fiber conducting technique, it reduces the interference between excitation light and acceptance light and also increases the ratio between signals and noises so as to improve the accuracy of ct value. the following graph is the amplification result of templates with 5,000 and 10,000 copy numbers on line-gene . 

Software

Absolute quantification
After quantitative analysis for the standards, the instrument will get a standard curve related to ct value and copy number. Combining standard curve with the CT value of unknown samples, the instrument can count the original copy number of unknown samples by quantitative analysis. Line-gene has a wide linear detecting range, which can detect  copies. The graph above is a result of a series of standards amplified by LineGene . the standards are seven(7) grades of gradient diluted egf samples with known concentration. the probe is labeled with fam. and the graph below is the standard curve that instrument gets. 

Relative quantification
When doing the relative quantification, the instrument doesn't make the standard curve, however, it offers the relative expression level of samples by comparing to inner standards. The relative quantitative detection procedure and result analysis are all finished automatically by software of LineGene. 

SNPS detection
LineGene system is a multicolor fluorescence detection system and can carry out the detection analysis of snps.

Meltin curve analysis
LineGene system can do melting curve analysis and finish such experiments as qualifying, identifying and mutation analysis.

Program setting - easy operation
The program setup interface offers easy operation of program setting and data presentation.

Program running - intuitive process
This interface offers the intuitive graphs so to monitor the PCR process Real-time.

Powerful analyzing functions
The analysis interface offers easy yet powerful functions for data analysis. It provides many automatic analyzing options, including quantitative analysis and melt-curve analysis.